Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

缺氧诱导因子-α和血管内皮素-1及血管内皮生长因子在胃肠道间质瘤中的表达及意义

目的探讨缺氧诱导因子-1α（HIF-1α）、血管内皮素-1（ET-1）和血管内皮生长因子（VEGF）在胃肠道间质瘤（GIST）中的表达关系及其对患者预后判断的价值。方法收集2003年1月至2006年12月中南大学湘雅医院（50例）、湖南省邵阳市中医院（36例）行手术治疗的86例GIST患者肿瘤标本,采用免疫组织化学染色方法检测标本中HIF-1α、ET-1和VEGF的表达。计数资料采用r检验,应用Spearman等级相关分析处理HIF-1α、ET-1和VEGF之间的相关性。Kaplan—Meier法计算患者生存率,生存分析比较采用Log—rank检验。结果86例患者中,HIF-1仅阴性23例、阳性30例、强阳性33例,阳性表达率为73．3％（63／86）;ET-1阴性29例、阳性28例、强阳性29例,阳性表达率为66．3％（57／86）;VEGF阴性24例、阳性26例、强阳性36例,阳性表达率为72．1％（62／86）。HIF-1α、ET-1和VEGF的表达两两均呈正相关（r=0．594,0．655,0．347,P〈0．05）;上述3种检测指标与GIST的美国国立卫生研究院（NIH）危险度分级、浸润转移、肿瘤直径和核分裂象有关（,=15．565、10．841、12．574、21．125,8．171、10．002、10．354、17．317,14．710、16．230、11．116、18．886,P〈0．05）;而与患者的性别、年龄、肿瘤原发部位无关（X2=0．059、0．071、5．070,0．468、0．074、1．665,0．231、0．370、3．712,P〉0．05）;进-步分析发现：上述3种检测指标阳性表达率随GISTNIH危险度分级的升高、出现浸润转移和肿瘤直径的增大而增加。86例患者随访率为91．9％（79／86）,3、5年生存率分别为82％和69％。中位随访时间为40．2个月（2—66个月）。HIF-1仪、ET．1和VEGF阳性患者的5年生存率分别为42％、45％和42％,显著低于阴性患者的64％、65％和63％（,=6．325,6．124,6．287,P〈0．05）。结论HIF-1d、ET-1和VEGF在GIST中的表达两两呈正相关,这3种检测指标提示肿瘤的恶性程度及患者预后。     

缺氧诱导因子-1α和血管内皮素-1及血管内皮生长因子在胃肠道间质瘤中的表达及意义

目的 探讨缺氧诱导因子-1α(HIF-1α)、血管内皮素-1(ET-1)和血管内皮生长因子(VEGF)在胃肠道间质瘤(GIST)中的表达关系及其对患者预后判断的价值.方法 收集2003年1月至2006年12月中南大学湘雅医院(50例)、湖南省邵阳市中医院(36例)行手术治疗的86例GIST患者肿瘤标本,采用免疫组织化学染色方法检测标本中HIF-1 α、ET-1和VEGF的表达.计数资料采用x2检验,应用Spearman等级相关分析处理HIF-1α、ET-1和VEGF之间的相关性.Kaplan-Meier法计算患者生存率,生存分析比较采用Log-rank检验.结果 86例患者中,HIF-1α阴性23例、阳性30例、强阳性33例,阳性表达率为73.3％(63/86);ET-1阴性29例、阳性28例、强阳性29例,阳性表达率为66.3％ (57/86);VEGF阴性24例、阳性26例、强阳性36例,阳性表达率为72.1％(62/86).HIF-1α、ET-1和VEGF的表达两两均呈正相关(r=0.594,0.655,0.347,P＜0.05);上述3种检测指标与GIST的美国国立卫生研究院(NIH)危险度分级、浸润转移、肿瘤直径和核分裂象有关(x2=15.565、10.841、12.574、21.125,8.171、10.002、10.354、17.317,14.710、16.230、11.116、18.886,P＜0.05);而与患者的性别、年龄、肿瘤原发部位无关(x2=0.059、0.071、5.070,0.468、0.074、1.665,0.231、0.370、3.712,P＞0.05);进一步分析发现:上述3种检测指标阳性表达率随GIST NIH危险度分级的升高、出现浸润转移和肿瘤直径的增大而增加.86例患者随访率为91.9％(79/86),3、5年生存率分别为82％和69％.中位随访时间为40.2个月(2～66个月).HIF-1α、ET-1和VEGF阳性患者的5年生存率分别为42％、45％和42％,显著低于阴性患者的64％、65％和63％(x2=6.325,6.124,6.287,P＜0.05).结论 HIF-1α、ET-1和VEGF在GIST中的表达两两呈正相关,这3种检测指标提示肿瘤的恶性程度及患者预后.     

内外合治促进腹部外科术后胃肠功能恢复临床观察

2002年6月—2002年11月我们采用芪朴汤加双侧足三里穴外敷黄香膏,对腹部外科术后胃肠功能恢复情况进行了观察,并与采用西医常规治疗的西药组、常规治疗加服芪朴汤的中药组比较,现将结果报告于下。 临床资料 150例患者均于硬膜外麻醉下行手术治疗,依入院顺序随     

缺氧诱导因子-1α和内皮素-1在胃肠道间质瘤中的表达及意义

目的 探讨缺氧诱导因子(HIF)-1α,血管内皮素(ET)-1在胃肠道间质瘤(GISTs)中的表达及其在预后判断中的价值.方法 应用免疫组织化学SP法对76例GISTs检测,观察HIF-1α、ET-1在GISTs中的表达情况.结果 HIF-1α、ET-1在GISTs中的阳性表达率分别为73.68%(56/76)、65.79(50/76),两者的表达呈正相关(P<0.05),其与胃肠道间质瘤的组织学分级、肿瘤直径、浸润转移和核分裂数有关(P<0.05),而与患者的年龄、性别、肿瘤原发部位无关(P>0.05);HIF-1α、ET-1在极低和低危险,中度危险和高危险及直径<2 cm和直径>5 cm组间差异有统计学意义(P<0.05),阳性表达率随恶性程度的增加和肿瘤直径的增大而增加(P<0.05),在有浸润转移和核分裂数≥5/50HP组中的表达明显高于无浸润转移和核分裂数<5/50HP组中的表达(P<0.05).结论 HIF-1α、ET-1在GISTs中的表达有联合倾向,可能提示肿瘤的恶性程度及其预后,并可作为判断预后的有用标志物.     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

中低位直肠癌保肛手术治疗的临床疗效观察

目的探讨中低位直肠癌保肛手术治疗的远期疗效及保肛手术治疗中低位直肠癌的原则。方法回顾性分析1997年3月至2003年9月收治455例中低位直肠癌的手术方式和随访资料。结果中低位直肠癌的手术方式中,保肛组与联合组患者在年龄、性别、肿瘤大小、组织学类型、分化程度、Dukes分期、手术时间、术后并发症、局部复发率、生存率上比较差异均无显著性。保肛组肿瘤切除后肛提肌上直肠残端的平均长度为(4.03±2.46)cm,联合组为(2.38±1.75)cm(P<0.01)。结论在根治性切除的前提下,保肛组生存率并不比联合组低,局部复发率并不比联合组高,且生活质量明显提高。适当扩大盆腔内切除范围是减少低位直肠癌术后局部复发的关键。保肛手术治疗中低位直肠癌并不影响远期疗效。     

MCT1在胰腺癌中表达水平及意义

目的 检测人胰腺癌中MCT1的表达水平,探讨其表达与胰腺癌临床病理学特征的关系.方法 应用免疫组化方法检测80例胰腺癌组织及40例正常组织切片中MCT1的表达水平,并统计分析MCT1的表达与临床病理特征的相关性.结果 80例胰腺癌标本中MCT1表达的总阳性率为76.25%,40例正常胰腺组织中MCT1表达的总阳性率为2...