Recombinant human granulocyte colony-stimulating factor after allogeneic bone marrow transplantation.

ＲＥＣＯＭＢＩＮＡＮＴＨＵＭＡＮＧＲＡＮＵＬＯＣＹＴＥＣＯＬＯＮＹ－ＳＴＩＭＵＬＡＴＩＮＧＦＡＣＴＯＲＡＦＴＥＲＡＬＬＯＧＥＮＥＩＣＢＯＮＥＭＡＲＲＯＷＴＲＡＮＳＰＬＡＮＴＡＴＩＯＮＨｕａｎｇＸｉａｏｊｕｎ黄晓军，ＧｕｏＮａｉｌａｎ郭乃榄，ＦａｎＹｕ...     

虞姬·霸王

裙袂生风颜散芳,豪气冲天自难忘。彩蝶单飞衣依旧,小楼已空无霸王。伤!伤!伤!千年望尽,只道是霸王投江尽是英雄叹。梨园万曲,只听得虞姬悲啼悉数儿女肠。拔剑!拔剑!投江!投江!了断!了断!不亡!不亡!怎生得一个玲珑如玉,一个魁梧似钢,一个磐石决心,一个软弱肩膀。却到了繁花将尽时候,虞姬还是虞姬,霸王已非霸王。虞姬·霸王@池边树     

如何控制访问医院信息系统敏感数据

医院信息系统数据越来越重要，其中涉及到非常多的敏感数据更应成为医院重点保护的对象。但由于原有系统的权限控制并不十分严谨，数据密码控制不严，如何进行有效的数据访问控制是一个需要全面规划的工作。用户控制必须覆盖全面，严格控制权限，既不能阻止用户正常地访问数据库的数据，也不能将数据访问权限范围无限地放大。     

肝素抑制后发性白内障形成的实验研究

肝素抑制后发性白内障形成的实验研究上海第二医科大学新华医院眼科夏小平，陆道炎，王丽天，陈才根，吴念祖目前有关减少后发性白内障形成的方法涉及二个方面。手术方面包括：尽可能多地清除晶体皮质和前囊膜上皮细胞、术中冷冻后囊、现在中山医科大学第三临床学院眼科尽...     

ACTIVIT OF HUMAN PLACENTAL GAMMA GLOBULININ BLOCKING IMMUNE FUNCTIONS IN VITRO AND IN ABROGATING THE XENOGENEIC, LOCAL GRAFT-VERSUS-HOST-REEACTEON

In order to understand the mechanism of immunosuppression caused by infusion of placental gamma globulin (PGG) in patients with renal allografts, rheumatoid arthritis, and graft – versus –host disease (GCHD) following bone marrow transplantation (BMT) ,we have examined the effect of PGG in vitro and in a model of the xenogeneic , local graft –ver- sus – host reaction (LGVHR) .PGG inhibited lymphocyte proliferation in mixed lymphocyte cultures (MLC) (P<0.005) and depressed interleukin -2 (IL-2) levels in such cultures at 72 hours (P<0.01) . In contrast phytohemagglutinin (PHA) –and pokeweed mitogen (PWM) –induced T and B lymphocyte blastogenesis was not affected by such PGG treatment .PGG treatment .PGG neither decreased the [3H] TdR pulse incorporation in unstimulated lymphocytes nor affected cell viability .Cell cycle analysis by flow cytometry showed that PGG reduced the percentage of cells in S and G2, M phases during the MLC, but did not alter cell cycling during PWM-stimulated proliferation . An immunosuppressive effect of PGG on the LGCHR was tested in a model of intracutaneous transplantation of PGG –treat human lymphocytes into cyclophosphamide – immunosuppressed rats. Lymphoprep – separated human tonsillar lymphocytes were incubated with RPMI-1640 buffer containing：（1）PGG,4mg/ml,(2) human plasma albumin,4mg/ml,(3)mitomycin-C,25ug/ml, or (4) no additive. Cell of each preparation (3x107cells in 0.1ml) were injected intracutaneously into cyclophosphamide-treated male rats at separate abdominal locations. A fifth site received only the buffer solution. Five days after injection of cells ,each rat received [125 I]IUdR (10uCi) intraperitoneally and was killed after 5 hours. For each site of injection, the diameters of induration were measured and 125 I was counted . There was no difference between buffer – treated and a ibumin – treated groups either in the diameter of the area of induration (t=0.66;P>0.5)or in radioactive counts(t=0.22;P>0.05).In the PGG –treated group, the induration and radioactivity measurements were significantly less than in control groups (t=3.72 and P<0.1;t=2.62 and P<0.02,respectively ) Cytophilic antibodies in PGG were thought to inhibit an early phase of T cell activation, and not to be cytotoxicity .In the LGVHR, the immune response might be abrogated either by immuno- regulatory suppression of T cell function or by toxicity to the infused lymphoid cells. For some clinical purposes, immuno- modulating, human antibodies might be preferred to murine, monoclonal antibodies.     

软性人工晶体和白内障超声乳化小切口植入

作者最近应世界人工晶体基金会的邀请,在美国作了短期参观学习。在此期间,通过交谈、直接观看手术和录像以及近期的文献资料,得到一个深刻的印象,即软性人工晶体作为一种新材料、新类型和新方法正在兴起。软性人工晶体可折叠起来,通过超声乳化术3mm的小切口植入眼内,伤口只需缝合一针。更新的研究表明,今后有     

T淋巴细胞亚群检测在呼吸疾病中的应用

肺作为一个免疫器官已日益受到国内外肺科学者的重视,通过支气管肺泡灌洗术(BAL),可获得肺的免疫活性细胞,用OKT系列单克隆抗体检测T淋巴细胞亚群的数量、比值,可估计体内免疫调节平衡状态及某些呼吸疾病的诊断、分期及指导治疗,且不同疾病有不同的数     

糖尿病患者人工晶体植入术

糖尿病是一种常见病,它已不再是人工晶体植科术的禁忌证。单纯糖尿病患者植入后房型人工晶体的术后视力与非糖尿病患者无明显差异;有视网膜病变的糖尿病患者在植入人工晶体时联合玻璃体切割术和全视网膜内光凝也能获得显著的视力改善。本文从患者选择、人工晶体选择、手术方式、手术效果、术后并发症及其防治等方面对该手术进行了综述。     

男性更年期的概念

近年 ,男性更年期的话题 ,已引起全社会的高度关注。在日本 ,由熊本悦明教授于 2 0 0 1年组建了日本AgingMale研究会 ,正在临床各领域进行着深入研究。本专辑由各领域的一线医学专家们执笔 ,反映了当前针对男性更年期的研究成果。非常值得一读。专辑选译自医学のあゆみ 2 0 0 3年 5月 1 0日发行的第Vol2 0 5,No .6特集———男性更年期。全集由大连大学医学院周吉海副教授主译、主审     

血浆巨细胞病毒多聚酶链反应检测用于指导干预性治疗

目的：分析用血浆巨细胞病毒（cytomegalovirus,CMV)多聚酶链反应（plymerase chain reaction,PCR)检测结果指导抗CMV干预性治疗的临床意义。方法：1999年8月至2001年7月行异基因造血干细胞移植（hematopoietic stem cell transplantation,HSCT)的所有患者自预处理开始，常规用PCR法检测血浆的CMV－DNA（采用华美公司生产的巨细胞病毒4℃ PCR检测试剂盒），每周1次。其中尚无CMV病的临床表现而检测到血浆CMV阳性的患者89例。随机选取52例阳性患者给予干预性治疗。将上述89例患者分为3组（分别为停止治疗时血浆CMV－PCR转阴组、停止治疗时未转阴组、血浆CMV阳性未治疗组）比较各组CMV病的发生率。CMV病的发生率采用Kaplan-Meier曲线表示，曲线间的比较用Log-Rank（曲线无交叉）或Breslow（曲线交叉）检验，某时点率的比较采用RXC列联表或χ^2检验。结果：100d内CMV病的发生率，在治疗后血浆CMV－PCR由阳性转为阴性组为10．26％，治疗结束时血浆CMV－PCR仍为阳性组为66.67%，血浆CMV－PCR阳性未治疗组为36．24％（P＝0.0000)。上述3组中任两组移植后100d CMV病的发生率差异均有显著性。移植后1年时上述3例CMV病累积发生率分别为30.65%、75.00%、42.95%(P=0.0009)，治疗未转阴组与治疗转阴组和阳性未治疗组相比，CMV病的发生率明显升高。结论：血浆CMV－PCR检测结果用来指导干预性治疗有一定临床意义。干预性治疗后血浆CMV－DNA仍未转阴者，应考虑换药或联合用药治疗。