Upregulation of voltage-activated potassium channels in hippocampus of Aβ25.35-treated rats

AIM: Potassium channels dysfunction has been indicated in Alzheimer disease. In the present study, the mRNA and protein expression alterations and the functional changes ot VOltage- activated potassium channels were studied in rat hippocampus after a single intracerebro- ventricular injection of β-amyloid peptide 25-35 (Aβ25.35). METHODS: The expressions of mRNA     

Effects of 2-（1-hydroxypentyl）-benzoate on platelet aggregation and thrombus formation in rats

AIM: Potassium 2-(1-hydroxypentyl)-benzoate (dl-PHPB),derivated from 3-n-butylphthalide (NBP), is a newly synthesized compound which is under development as a therapeutic drug for cerebral ischemia. In the present study, we used ex vivo platelet aggregation method and in vivo arteriovenous shunt model to evaluate the antiplatelet and antithrombotic effects of d/-PHPB. METHODS: In the test of platelet aggregation, dl-PHPB was     

Possible neuroprotective role of arachidonic acidsensitive potassium channels in rat acute cerebral ischemia

AIM: To explore the actual role of arachidonic acid-sensitive potassium channels including TREK- 1, TREK-2 and TRAAK in acute cerebral ischemia. METHODS: In acute rat MCAO model, RT-PCR and Western blot analysis were used to investigate the expression changes of TREK. Immunohistochemistry experiments were also used to study their protein expressions in vivo. RESULTS: TREK-1, TREK-2 and TRAAK mRNA expression     

Effects of temperature and intracellular sodium, ATP and pH on Na^＋-Ca^2＋ exchange currents of intact guinea-pig myocytes

AIM: The Na^ -Ca^2 exchange is a major pathway for removal of cytosolic Ca^2 in cardiac myocytes. There have been many re-searches reporting about the effects of temperature, intracellular sodium, ATE and pH on Na^ -Ca^2 exchange currents. But most of these researches were made with giant-patch voltage-clamp technique or with Ca^2 flux studies in sarcolemmal vesicles. During     

Role of delayed rectifier K channel currents in β-amyloid （1-40）-induced caspase-3 activation and apoptosis in primary cultured cortical neurons

AIM: To investigate the effect of chronic exposure to/]-amyloid（1-40)(Aβ1-40) on delayed rectifier K^ currents (IK) in primary cultured ratcortical neurons and the effect of IK on caspase-3 activation and neurons apoptosis induced by Aβ1-40. METHODS: K^ currents were recorded using whole-cell patch clamp techniques; cell sviability rate and apoptosis were studied using MTT and Hoechst     

A model of subarachnoid hemorrhage in rats

AIM: To build a simple and repeatable animal model of subarachnoid hemorrhage (SAH). METHODS: SAH was introduced by passing a nylon thread up through the right internal carotid artery and piercing a hone in the right anterior cerebral artery. At 12 and 24 h, the rats were evaluated with rotarod test and the behavior scale (5-point scale). RESULTS: The ratswere trained through rotarod test and then randomly divided into