GIRK1在颞叶癫痫大鼠海马齿状回中的表达及其意义

目的探讨GIRK1在颞叶癫癎大鼠海马齿状回的表达及其意义.方法112只雄性SD大鼠随机分为实验组(n=70)与对照组(n=42),同时建立海人藻酸(KA)颞叶癫模型.选取KA腹腔注射后3、6、12、24、48 h,7、30 d为研究的时间点.用原位杂交法及免疫组织化学法检测海马齿状回GIRK1 mRNA及蛋白的表达.结果实验组大鼠海马齿状回GIRK1 mRNA表达在致癎后6 h较对照组减少,而在致癎后至7～30 d较对照组增高.结论在颞叶癫癎的不同时期海马齿状回GIRK1表达的变化反映出颞叶癫癎的复杂性.     

加快中医药事业发展为富民强省贡献力量──访湖南省中医药研究院院长蔡光先教授

中医药是中华民族优秀文化的瑰宝。中医药事业是我国卫生事业的重要组成部分,中药产业是我国具有原创知识产权的优势民族产业。中药以其天然、具有治疗、预防、保健等作用,且毒副作用小而享誉世界。不久前召开的全国中医药工作会议也进一步阐明:“具有几千年悠久历史的中医药,在构建社会主义和谐社会和全面建设小康社会进程中充满着无限生机与活力。继承发展中医药是历史赋予我们的神圣职责和光荣使命”。那么,在全国久负盛名的湖南中医药行业,在新的历史时期将怎样审视和致力于中国中医药事业发展,如何为构建和谐湖南和加快富民强省作出贡献?笔者近日在湖南省中医药研究院隆重庆祝建院50周年的喜庆日子里,访问了湖南中医药大学党委书记、湖南省中医药研究院院长、博士生导师蔡光先教授。问:50年风雨征程,50年艰苦创业。请问您,走过半个世纪历程的湖南省中医药研究院,取得了哪些重大成就?答:湖南省中医药研究院从建院之日起,始终站在中国中医药事业发展和科学研究最前沿,面向国家战略和社会发展需要,面向人民群众健康需求,在中医药科研、医疗、教育、生产、信息交流等领域,谱写了一篇篇精彩华章,取得了一项项丰硕成果,成为中华杏林一朵璀璨夺目的奇葩。具体来讲,取得了以下...     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

肱骨小头完全脱位8例报告

我院及本人援外期间共收治肱骨小头完全骨折8例。8例中女2例、男6例;年龄30～40岁;均有外伤史,5例手法复位加石膏托固定,2例手术、1例拒绝手术。经随访7例疗效均满意。     

“脚穴”按摩可治神经衰弱

《科学养生》杂志曾发表过我写的“我用‘脚穴’按摩的方法治好了王淑琴的神经衰弱”一文，引起读者的广泛关注。现将按摩方法和注意事项介绍如下。     

医学课程线上教学现状与启示——以中南大学湘雅医学院为例

目的　通过问卷调研方式,拟从整体上了解新型冠状病毒肺炎疫情期间医学生对医学课程线上教学的认识和线上学习现状,并对医学课程满意度及其影响因素进行分析。方法　调查对象为中南大学湘雅医学院在读医学生188人,采用自编问卷匿名调研。运用SPSS 26.0软件对相关数据进行分析。计数资料采用率表示,组间比较采用t检验或方差分析,检验水准α=0.05。结果　88.83%（167人）的学生认为线上教学有必要或很有必要,可自由安排学习时间为线上教学最大的优点。而59.57%（112人）的学生认为学习自觉性降低,49.47%（93人）的教师对教学平台不适应,59.57%（112人）的教师对教学方式改变不适应,可能是影响教学质量的最主要原因。结论　学生对疫情期间开展的医学课线上教学普遍认可度高,但仍存在学生自律性降低、师生互动不够、对部分网课平台建设不满意、无法亲临实验室和参与临床实践等问题。本次调研对后疫情时代及未来的医学教育模式修订仍可起到帮助作用,推荐医学理论课和PBL讨论课可采用线上线下混合式教学,同时应加强临床实践和医学实验课的网络学习资源建设。     

铁及氧化应激在多发性硬化中的作用机制及其MRI研究进展

多发性硬化(multiple sclerosis,MS)是中枢神经系统最常见的一种炎性脱髓鞘疾病,其发病机制迄今不明。近年来研究表明铁和氧化应激均参与到MS的发病机制中,但其具体的时间空间动态变化规律尚未完全被阐明。因此,借助多参数MRI进行在体无创性定量评估MS病灶内的铁及氧化应激水平,对揭示MS各期不同病理特征具有重要意义。作者就铁及氧化应激在多发性硬化中的潜在致病机制和相应MRI研究进展进行综述。     

连接蛋白43在骨关节炎发病机制中的研究进展

骨关节炎(osteoarthritis,OA)是常见的关节疾病,也是导致残疾的主要原因之一,给患者及家庭带来巨大的经济生活负担。虽然OA的发病机制至今尚未完全明确,但关节软骨代谢平衡的破坏被认为与骨关节炎的发生息息相关。研究发现连接蛋白43(connexin43)在维持关节软骨代谢平衡的稳态中发挥着至关重要的作用,它所参与形成的半通道(hemichannels)和缝隙连接(gap junctions)在细胞与细胞外基质,以及细胞之间建立起直接的物质信号交换通道。同时,连接蛋白43的高表达和(或)分布的变化都将影响软骨细胞结构和功能的完整性。因此,连接蛋白43被认为与骨关节炎的发生有着密切关系。本文就连接蛋白43与骨关节炎发病机制的研究进展做一综述。     

东革阿里不同提取物抗高尿酸血症的实验研究

摘 要 目的：考察东革阿里不同提取物对高尿酸血症大鼠尿酸排泄以及肾脏功能的影响。方法: 将60只雄性Wistar大鼠均分为空白组、模型组、阳性对照组、氯仿组、乙酸乙酯组和正丁醇组,灌胃大鼠酵母膏、腺嘌呤和氧嗪酸钾制作大鼠高尿酸血症模型。造模成功后,除空白组以外的其他组继续给予造模剂,除模型组外的其他组在给予造模剂1h后分别给予相应的药液,给药14 d,检测大鼠血清和尿液中的尿酸（UA）、尿素氮（UN）及肌酐（Cr）水平、肝脏及血清中的黄嘌呤氧化酶（XOD）活性等指标。结果:与模型组相比,氯仿组和正丁醇组血清尿酸、肌酐水平显著降低（P<0.05）,尿液尿酸、肌酐水平显著升高（P<0.05）,XOD活性显著降低（P<0.01）。与阳性对照组相比,氯仿组、乙酸乙酯组、正丁醇组血清和尿UA、Cr、UN水平以及血清和肝脏XOD活性等指标均无显著性差异（P＞0.05）。氯仿组、乙酸乙酯组、正丁醇组血清和尿UA、Cr、UN水平以及血清和肝脏XOD活性等指标均无显著性差异（P＞0.05）。结论:东革阿里氯仿萃取物、正丁醇萃取物能降低腺嘌呤、酵母膏和氧嗪酸钾引起的高尿酸血症大鼠血尿酸水平,可能是通过抑制黄嘌呤氧化酶活性来发挥降尿酸作用。