用(51)~Cr标记蛙红细胞技术测定家兔心肾局部血流量的实验研究

用(51)~Cr标记蛙红细胞作为一种新型指示剂—生物微球,测定麻醉家兔心、肾局部血流量,所得结果与碳化微球一致。此一技术简便易行,准确可靠,重复性强,可在一定范围内代替碳化微球,广泛应用于测定心输出量以及组织、器官局部血流量的动物实验中。     

用生物微球技术测定 3Gz对麻醉兔内脏器官血流的影响

将~(51)Cr标记蛙红细胞技术应用于加速度生理学研究,测定 3Gz对麻醉兔内脏器官血流的影响。结果表明, 3Gz时麻醉兔冠状血流无明显改变,而肝、胃、肠、肾脏皮质和髓质的血流则明显减少,其中肾皮质血流的减少尤重较对照组竟减少了96.57％。研究表明,生物微球技术可用于加速度生理学研究。     

差示分光光度法测定血浆中甲硝唑

本文报导用10%三氯醋酸(TCA)液既作血浆蛋白沉淀剂又作甲硝唑提取溶剂,在320nm处测定沉淀血浆蛋白后的10%TCA液与其碱液的吸收度差的差示分光光度法。本法操作简便,灵敏度高,重现性较好,可排除血浆中紫外吸收物质的干扰,符合临床药液监测及药代动力学研究的要求。根据测定动物体内血药浓度甲硝唑的药动学过程符合二室模型。     

用微球技术测定+3Gz对麻醉兔心、脑血流的影响

用放射性同位素~(51)Cr标记蛙红细胞,测定+3Gz对麻醉兔心、脑局部血流量的影响。在+3Gz应激时,心肌血流量无明显变化;脑血流量减少,左、右大脑分别减少49.09％和48.15％,小脑减少45.78％,而延脑只减少17.44％。表明在+3Gz影响下,各部脑组织血流量的减少不是均一的,而是产生了脑血流的再分配。     

Measurement of Regioual Blood Flow of Organs in Aesthetized Rabbits with Biological Microsphere Technique during 3Gz Stress

This article states that the tachnique of ~(51)Cr-labelled frog redblood cells can be applied to studying acceleration physiology tomeasure the effects of 3Gz acceleration on regional blood flow oforgans in anesthetized rabbits.The results show that the coronaryblood flow of anesthetized rabbits was unchanged during 3Gzstress.However,the blood flow of the brain,liver,stomach,smallintestines,renal cortex and medulla was markedly reduced,especially,that of the renal cortex was decreased by 96.57% as compared withthe control group.The study indicates that it is appropriate to applybiological microsphere technique to the study of acceleration physi-ology.     

Gz对兔眼视网膜组织形态学的影响

为观察十Gz对视网膜组织形态学的影响，对暴露于十5Gz60s的家免神网膜组织进行了光镜及电镜观察。结果表明，＋5Gz作用60s对兔眼视网膜组织形态学无明显改变。说明本实验条件不足以使兔眼视网膜组织形态学发生改变。