平阳霉素(博菜霉素A5)对同步化的CHO细胞的敏感周期时相的研究

平阳霉素(博莱霉素A₅)作用于同步化的各个周期时相的CHO细胞。结果用集落形成率来表示。研究表明平阳霉素对M期细胞最为敏感,其敏感顺序为M>G₂>S>G₁。实验使用的细胞同步化方法是用有丝分裂选择法收集M期细胞;用2 mMTdR双阻断法获得S期同步化细胞,再由M期和S期细胞分别向后推移1.5～2小时和7小时以获得G₁期和G₂期的同步细胞,并附以有丝分裂指数(MI)和标记指数(LI)检测各周期时相细胞的同步化程度。     

抗阿霉素的中国仓鼠卵巢上皮细胞系的抗药性

用细胞生物学和ＳＤＳ－ＰＡＧＥ电泳方法，研究抗阿霉素ＣＨＯ细胞系的特性，用间接免疫荧光法，未测到ＲＣ１超表达Ｐ－糖，ＲＣ１降低Ｄｏｘ的摄入，增加膜的流动性，Ｄｏｘ在ＣＨＯ中主要分布在细胞核，在ＲＣ１中则分布在细胞质和细胞核，ＲＣ１中有一种分子质量为３０－４０ＫＤＡ的核蛋白的表达被抑制，而在敏感ＣＨＯ中其表达正常。     

人早幼粒白血病HL60细胞及其亚系中mdr—1基因和癌基因表达的关系

研究人早幼粒白血病ＨＬ６０细胞系及其分别抗Ｈａｒ，Ｈａｒ＋Ｖｅｒ，ＲＡ的抗药性细胞亚系中癌基因与多药抗性表达的相关性。     

粉防己碱和蝙蝠葛碱减低抗三尖杉酯碱的人白血病HL60细胞对阿霉素的抗性(英文)

目的:研究粉防己碱(Tet)和蝙蝠葛碱(Dau)能否减低抗三尖杉酯碱(Har)的人早幼粒白血病HL60抗性株对阿霉素(Dox)的抗性。方法:细胞计数法和克隆形成法测定药物毒性,流式细胞光度术分析细胞周期变化,荧光法测定Dox含量。结果:无细胞毒性的Tet和Dau明显地增强Dox对HL60抗性细胞的生长抑制作用,使克隆形成率从Dox单药的60％分别降低到0.2％,9.2％,使阻断在G_2M期的抗性细胞增多,但Tet和Dau不增强Dox对敏感的HL60细胞的毒性。Tet使胞内Dox积聚增加。结论:Tet和Dau减低抗Har的HL60细胞对Dox的抗性,其机制是增加Dox在细胞内积聚。     

EFFECTS OF BLEOMYCIN A5 COMBINED WITH CALMODU-LIN INHIBITOR ON THE PROLIFERATION OF S-180 CELLS IN VITRO

The effects of bleomycin A5 (BLM A5) alone and combined with calmodulin inhibitor N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide (W-13) on the proliferation on S-180 cells in vitro were studied. IC50 of BLM used alone for the cells was about 2.63 μg/ml, but it was reduced to 1/3.8 and 1/9.5 of 2.63 μg/ml when plus W-13 1, 5 μg/ml respectively. The results indicated that nontoxic doses of W-13 enhanced the hinibition of cell proliferation under the condition of BLM 0.5 - 2.5 μg/ ml. In colony forming test, the survival fraction of S-180 cells treated with BLM plus W-13 was decreased to 1/87 - 240 of that of the cells treated with BLM alone. The results suggest that W-13 can enhance antitumor activity of BLM in vitro and may be used as an synergist of BLM A5 in vivo.