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Panawan Suttiarporn Watcharapong Chumpolsri Sugunya Mahatheeranont Suwaporn Luangkamin Somsuda Teepsawang Vijittra Leardkamolkarn 《Nutrients》2015,7(3):1672-1687
Structures of some bioactive phytochemicals in bran extract of the black rice cv. Riceberry that had demonstrated anti-cancer activity in leukemic cell line were investigated. After saponification with potassium hydroxide, separation of the unsaponified fraction by reversed-phase high performance liquid chromatography (HPLC) resulted in four sub-fractions that had a certain degree of anti-proliferation against a mouse leukemic cell line (WEHI-3 cell), this being IC50 at 24 h ranging between 2.80–467.11 μg/mL. Further purification of the bioactive substances contained in these four sub-fractions was performed by normal-phase HPLC. Structural characterization by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance spectroscopy (NMR) resulted in, overall, the structures of seven phytosterols and four triterpenoids. Four phytosterols, 24-methylene-ergosta-5-en-3β-ol, 24-methylene-ergosta-7-en-3β-ol, fucosterol, and gramisterol, along with three triterpenoids, cycloeucalenol, lupenone, and lupeol, were found in the two sub-fractions that showed strong anti-leukemic cell proliferation (IC50 = 2.80 and 32.89 μg/mL). The other sterols and triterpenoids were campesterol, stigmasterol, β-sitosterol and 24-methylenecycloartanol. Together with the data from in vitro biological analysis, we suggest that gramisterol is a significant anti-cancer lead compound in Riceberry bran extract. 相似文献
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Buayairaksa M Kanokmedhakul S Kanokmedhakul K Moosophon P Hahnvajanawong C Soytong K 《Archives of pharmacal research》2011,34(12):2037-2041
Chemical investigation of fungal biomass of the fungus Syncephalastrum racemosum led to the isolation of new natural products (3R),(5S)-5-hydroxy-de-O-methyllasiodiplodin (1), 6-oxode-O-methyllasiodiplodin (2), in addition to five known compounds, de-O-methyllasiodiplodin (3), lasiodiplodin (4), (3R),(5R)-5-hydroxy-de-O-methyllasiodiplodin (5), ergosterol (6), and ergosterol peroxide (7). Their structures were elucidated by spectroscopic techniques. The absolute configuration of 1 was determined by a modified Mosher’s method. Compound 1 showed cytotoxicity against cholangiocarcinoma, KKU-M139, KKU-M156, and KKU-M213 cell lines with IC50 values in the range of 14–19 μg/mL, while 3 showed cytotoxicity against KB, BC1, and NCI-H187 cell lines with IC50 values of 12.67, 9.65, and 11.07 μg/mL, respectively. 相似文献
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Three new diarylpropanes (1-3), a new arylpropyl quinone (4), and the known 1-(2-hydroxy-4-methoxyphenyl)-3-(4-hydroxy-3-methoxyphenyl)propane (5) were isolated from a methanol extract of stems of Combretum griffithii. Their structures were elucidated by spectroscopic methods. Compounds 1, 2, 4, and 5 showed cytotoxicity against one or more cancer cell lines (KB, MCF7, and NCI-H187), and compound 5 exhibited activity against Mycobacterium tuberculosis (MIC 3.13 μg/mL). 相似文献
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Orasa Panawan Atit Silsirivanit Chih-Hsiang Chang Siyaporn Putthisen Piyanard Boonnate Taro Yokota Yuki Nishisyama-Ikeda Marutpong Detarya Kanlayanee Sawanyawisuth Worasak Kaewkong Kanha Muisuk Sukanya Luang Kulthida Vaeteewoottacharn Ryusho Kariya Hiromu Yano Yoshihiro Komohara Kunimasa Ohta Seiji Okada Sopit Wongkham Norie Araki 《Cancer science》2023,114(8):3230-3246
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