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排序方式: 共有615条查询结果,搜索用时 31 毫秒
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2.
Zhang Y Siebert R Matthiesen P Harder S Theile M Scherneck S Schlegelberger B 《Virchows Archiv : an international journal of pathology》2000,436(3):271-275
For the first time, combined immunophenotyping and fluorescence in situ hybridization (FISH) technique according to the ”fluorescence
immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasms” (FICTION) technique have been successfully
applied in solid tumors. Thus, we were able to visualize the antigen expression of cells with chromosomal deletions of a tumor
suppressor region directly. In six breast carcinoma cell lines, we investigated the correlation between estrogen receptor
(ER) expression status and deletions of the estrogen receptor gene (ESR). To screen for deletions of the ESR gene, dual-color
FISH was performed with a YAC (yeast artificial chromosome) probe containing the ESR gene and, as internal control, with a
centromeric probe of chromosome 6. Deletions of the ESR gene were detected in four of six cell lines. For direct comparison
of ER expression with the copy number of the ESR gene at the single cell level, immunophenotyping with mouse anti-human ER
antibody was combined with FISH with the YAC probe containing the ESR gene according to the FICTION technique. There was no
correlation between lack of or reduced ER expression and deletions of the ESR gene. One cell line with deletions of the ESR
gene did express ER on the protein level, while another cell line without a deletion did not. Cells with deletions of the
ESR gene were either ER expression positive or negative. The staining intensity of ER expression was not associated with the
copy number of the ESR gene. Thus, this FICTION study unequivocally shows that deletions of the ESR gene are not the major
cause of absent or reduced ER expression in breast carcinoma cell lines.
Received: 6 September 1999 / Accepted: 14 September 1999 相似文献
3.
Spontaneous secretion of interleukin-4, interleukin-10 and interferon-gamma by first trimester decidual mononuclear cells 总被引:4,自引:0,他引:4
Ekerfelt C Lidström C Matthiesen L Berg G Sharma S Ernerudh J 《American journal of reproductive immunology (New York, N.Y. : 1989)》2002,47(3):159-166
PROBLEM: A T-helper cell type 2 (Th2) cytokine dominated microenvironment has been predicted to be crucial for successful pregnancy. However, little information is available about local cytokine secretion in the human decidua. We determined the spontaneous secretion of interleukin-4 (IL-4), interferon-gamma (IFN-gamma) and IL-10 by decidual mononuclear cells at the single cell level and compared it with their secretion by peripheral blood mononuclear cells (PBMC) in the first trimester of pregnancy. METHODS OF STUDY: The cytokine secretion from decidual and blood cells was detected by a sensitive enzyme-linked immunosorbent spot-forming cell (ELISPOT)-assay. RESULTS: Cells secreting IL-4 (median 153, range 8-530), IL-10 (median 188, range 32-1600) and IFN-gamma (median 123, range 15-1140) were detected in all decidual and blood samples. The cytokine secretion showed a co-linear pattern in both the blood and decidua, i.e. when one cytokine was secreted at high levels, the others followed the trend. No correlation was found between the number of cytokine secreting cells in blood and decidua for any of the cytokines. CONCLUSIONS: Interleukin-4 and IL-10 are locally secreted in the decidua early during normal pregnancy, probably counteracting the fetal rejecting effects of co-expressed IFN-gamma. The cytokine secretion by blood cells does not generally reflect the local secretion pattern during first trimester pregnancy. 相似文献
4.
5.
Leif Matthiesen Gran Berg Jan Ernerudh Leif Hkansson 《American journal of reproductive immunology (New York, N.Y. : 1989)》1996,35(2):70-79
PROBLEM: In normal pregnancy the maternal immune system should be directed towards tolerance or suppression in order not to reject the partly foreign feto-placental unit. The aim of this investigation was to find hallmarks of systemic immunosuppression during normal pregnancy. METHODS: Five healthy primigravidae were examined during pregnancy and postpartum with flow cytometric analysis to define T and B lymphocyte subsets in peripheral blood. In addition, we studied the proliferative response of lymphocytes to mitogens or interleu-kin-2 (IL-2) alone or in combination with immunomodulating drugs or interleukin-4 (IL-4). The results were compared to healthy, non-pregnant women. RESULTS: During pregnancy and early puerperium we noted an immune balance in favour of suppression, as measured by increased numbers of T “helper/suppressor” (CD4+ CD45RA+) and “suppressor”/effector T cells (CD8+S6F1-), and decreased numbers of T “helper/inducer” (CD4+CD29+), T “helper/memory” (CD4+CD45RO+), killer/effector T cells (CD8+S6F1+), and Natural Killer cells (CD56+), as well as decreased numbers of activated lymphocytes expressing IL-2 receptor (CD25+) and T cells expressing HLA-DR (HLA-DR+CD3+). During pregnancy, lymphocyte proliferation was impaired in autologous serum with concanavalin A (ConA), phytohemagglutinin (PHA), or IL-2. A difference in proliferative response to PHA or IL-2 between cultures with AB serum and autologous serum is suggestive of an immunosuppressor factor in serum during pregnancy. Indomethacin significantly increased lymphocyte proliferation in autologous serum with ConA, indicating PGE2 mediated suppressor activity during pregnancy. Chlorambucil and cimetidine modulated the proliferative response to ConA, indicating an alkylating agent sensitive and a histamine dependent suppressor activity during pregnancy. CONCLUSIONS: During normal pregnancy, a state of systemic suppression of the maternal immune system seems to be present. 相似文献
6.
The constitutional gain-of-function variant p.Glu1099Lys in NSD2 is associated with a novel syndrome
Bernt Popp Melanie Brugger Sibylle Poschmann Tobias Bartolomaeus Maximilian Radtke Julia Hentschel Nataliya Di Donato Andreas Rump Janina Gburek-Augustat Elisabeth Graf Matias Wagner Ina Sorge Johannes R Lemke Thomas Meitinger Rami Abou Jamra Vincent Strehlow Theresa Brunet 《Clinical genetics》2023,103(2):226-230
7.
Background
Phosphoinositides mediate one of the intracellular signal transduction pathways and produce a class of second messengers that are involved in the action of hormones and neurotransmitters on target cells. Thyroid hormones are well known regulators of lipid metabolism and modulators of signal transduction in cells. However, little is known about phosphoinositides cycle regulation by thyroid hormones. The present paper deals with phosphoinositides synthesis de novo and acylation in liver at different thyroid status of rats. 相似文献8.
9.
We report a case of acquired thrombotic thrombocytopenic purpura (TTP) in a 34-year old patient with a prior diagnosis of
systemic lupus erythematosis (SLE) who was recently started on hydroxychloroquine. Presenting symptoms included fevers, sore
throat and productive cough with progressive weakness, dyspnea on exertion, hemoptysis and dark urine. Initial laboratory
abnormalities were consistent with an acute microangiopathic hemolytic anemia and severe thrombocytopenia. At the time of
admission, the patient’s lupus was highly active as evident by his high SLE Disease Activity Index (SLEDAI) score. He was
later also found to have severely reduced ADAMTS-13 levels and a positive antibody assay. This case highlights the occasional
difficulty in pinpointing the exact etiology of TTP as well as establishes a possible novel drug association between hydroxychloroquine
and TTP development. 相似文献
10.