喜炎平注射液治疗急性病毒性咽炎疗效观察

目的观察喜炎平注射液治疗急性病毒性咽炎的临床疗效。方法将68例急性病毒性咽炎患者随机分为2组,对照组34例给予常规补液及抗生素治疗;治疗组34例在对照组治疗基础上加用喜炎平注射液静脉滴注,均以5 d为1个疗程。观察2组患者治疗前后临床症状、体征(包括退热时间,咳嗽、咽痛、咽部充血消失时间等)变化情况。结果治疗组总有效率(94%)明显高于对照组(79%)。且治疗组患者发热、咽痛、咳嗽、咽部充血消失时间明显短于对照组。结论喜炎平注射液治疗急性病毒性咽炎疗效好,疗程短,值得临床推广应用。     

佛手柑内酯对双氧水诱导人脐静脉内皮细胞衰老的影响

BACKGROUND: The effective treatment of traditional Chinese medicine can alleviate the aging of endothelial cells. Traditional Chinese medicine is difficult to extract, and in contrast, Bergapten is cheap and easily purified, but its antisenescence is little reported. OBJECTIVE: To explore the effect of Bergapten on H2O2-induced senescence of human umbilical vein endothelial cells. METHODS: Human umbilical vein cell aging model was induced by H2O2, and pretreated with 0.1, 1 and 10 μmol/L Bergapten, respectively. Expressions of pRb, pAmpk, pS6, LC3-II, and Beclin-1 protein in each group were detected by western blot assay, and the number of aging cells and pS6-positive cells was determined by β-galactosidase staining and immunofluorescence, respectively. ESULTS AND CONCLUSION: After Bergapten pretreatment, pS6 and pRb protein expressions were significantly downregulated, and the number of cells positive for β-galactosidase and pS6 was significantly decreased, while protein expressions of pAmpk, Beclin-1and LC3-II were significantly increased (P < 0.05). These findings suggest that Bergapten is able to delay the H2O2-induced aging of human umbilical vein endothelial cells 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

差异基因表达谱分析颈动脉不稳定斑块相关基因及可能的信号通路

目的探讨颈动脉不稳定斑块发生的分子机制。方法从ArrayExpress数据库下载基因表达谱数据E-MTAB-2055。该数
据包含24例颈动脉不稳定斑块和24例稳定斑块组织,从中筛选差异基因,并通过富集分析获取与不稳定斑块有关的生物学过
程和通路。同时,通过构建差异表达基因的生物网络来寻找与该疾病高度相关的风险模块,并用荧光定量PCR法检测五对不稳
定斑块和斑块旁标本,验证风险模块中部分基因的差异表达。结果不稳定斑块中发生显著性差异表达变化的基因共有439
个,其中上调基因为232个,下调基因为207个。涉及到的生物学过程和通路大部分与炎症和免疫应答有关。生物网络和模块
分析提示CXCR4、VCL和TYROBP 可能在不稳定斑块发生发展过程中发挥着重要作用。荧光定量PCR结果显示CXCR4和
TYROBP基因表达情况与芯片数据分析结果一致。结论本研究比较完整地揭示了不稳定斑块差异表达谱特征和所涉及的生
物学过程和信号通路,其中TYROBP可能是不稳定斑块发生发展进程中新的致病基因。