临床胃粘膜活检及胃癌手术标本纤维连结蛋白的定位观察

采用免疫组化及免疫电镜技术对临床胃粘膜活检及胃癌手术标本进行了纤维连结蛋白(FN)的定位观察。结果,FN见于胃粘膜上皮细胞和部分癌细胞内以及各种基底膜、间质中。肠化及异型增生上皮细胞FN染色增强,胃癌细胞和其基膜FN减少缺失且与癌细胞分化程度相关,胃癌间质FN丰富,尤其以癌浸润前缘明显。本文着重讨论了胃癌FN改变与癌细胞生物学特性的关系。     

醋酸铊中毒的病理变化——附一例尸检报告

口服醋酸铊治疗发病而致中毒已有临床报道,而醋酸铊中毒致死的病理变化在国内则未见报道。我们遇到一例,经过较详细的形态学观察,发现一些特徵性的病理变化,并从中探讨其中毒症状和死亡原因的发生原理,以供参考。患者,男性,13岁,曾服过量醋酸铊治疗发病。服药后一小时许即出现恶心,呕吐。随后陆续出现昏睡、小腿酸痛、上肢发麻、腹泻、便血、神志不清、视力模糊等症状,至服药后第九日来本院门诊求治,体检:体温36.5℃,脉搏100次/分,     

胃癌印戒细胞电镜酶细胞化学研究

目的对胃癌印戒细胞５种细胞器标志酶（ＡＬＰａｓｅ，ＡＣＰａｓｅ，Ｇ６Ｐａｓｅ，ＴＰＰａｓｅ和ＣＣＯａｓｅ）进行超微水平的定位观察，探讨胃癌印戒细胞的生物学行为及其发生机理．方法应用电镜酶细胞化学技术对５例印戒细胞癌，２例伴有印戒细胞的粘液腺癌进行５种细胞器标志酶的电镜观察．结果胃癌印戒细胞粘液的合成、分泌和消化功能增强，相应细胞器数目增多，酶活性增强，其中尤以ＡＣＰａｓｅ，ＴＰＰａｓｅ为显著．癌周间质缺乏胶元纤维．结论胃癌印戒细胞在功能上属高分化，但其形态与功能不协调，出现了畸形分化．其侵袭力强、转移率高的原因与癌细胞分泌的酶对细胞连接的影响和溶解癌周间质密切相关．     

胃粘液腺癌分泌细胞器电镜酶细胞化学研究

利用酶细胞化学方法对胃粘液腺癌细胞葡萄糖-6-磷酸酶(G6-Pase)焦磷酸硫胺素酶(TPPase)和酸性磷酸酶(AcP)进行了光、电镜 水平的定位观察。作者发现胃粘液腺癌G6Pase、TPPase反应较弱,AcP反应多明显增强;电镜下呈弱G6Pase反应的内质网常被挤至细胞周边部或夹于粘液颗粒之间,呈“点彩”状;TPPase反应除见于高尔基体扁平膜囊外,还见于一些未成熟分泌颗粒内,提示分泌颗粒的加工和分泌加速;癌细胞溶酶体数量增加,泌噬作用活跃,AcP反应明显.作者认为胃粘液腺癌分泌功能异常活跃与其畸形分化过程有关;溶酶体的大量增加为其易于转移提供了必要的形态学基础。     

CARCINOEMBRYONIC ANTIGEN EXPRESSION IN GASTRIC CANCER AS RELATED TO BIOLOGICAL BEHAVIOUR AND PROGNOSIS

Objective: To determine the relationship betweencarcincembryonic antigen(CEA)expression in gastriccancer and biological behaviour or prognosis.Material and Methods: Surgically resected speci-mens of gastric cancer from 104 patients were obtained.The content and distribution of CEA in gastric cancerwere studied by immunohistochemical staining andimmunoelectron microscopic technique. The relationshipbetween CEA in gastric cancer and biological behaviouror prognosis were evaluated.Results: The positivity of CEA Was significantlyhigher in the patients with advanced stage, vascularinvasion and lymph node metastasis than that in thepatients without. The S-year survival rate of thc CEA (-)group was significantly higher than that of the CEA ( )group. Among the patients with advanced stage orlymph node metastasis, the survival rate was higher in theCEA(-) group tban in the CEA( )group.Conclusions: Immunostaining for CEA in gastriccancer tissue may be helpful in differentiating amongtumors that appear similar by c     

胃癌癌胚抗原的分布型式与组织类型及组织发生分型的关系

本文应用免疫组化技术对104例胃癌组织CEA分布进行了观察。发现胃癌组织CEA有三种分布型式:即Ⅰ型(浆型),Ⅱ型(膜型)和Ⅲ型(弱反应型)。粘液癌以Ⅰ型分布为主,分化性癌以Ⅱ型分布为主,差分化癌三种分布型式均有。按组织发生分型,肠型胃癌主要显示Ⅱ型分布,胃型和干细胞型胃癌主要显示夏型分布。提示癌组织CEA分布型式可能与肿瘤分化程度有关,而癌组织CEA含量可能与组织发生有一定关系。     

ULTRASTRUCTURAL LOCALIZATION OF CARCINO- EMBRYONIC ANTIGEN IN GASTRIC CANCER IMMUNOELECTRON MICROSCOPIC OBSERVATION

The ultrastructural Iocalization of carcinoem. bryonic antigen (CEA) in 14 cases of gastric cancer was studied by immunoelectron microscopic tech- nique. There was little or no CEA on the microvilli of normal epithelial cells. In intestinal metaplastic epithelium, CEA was found on the microvilli of ab- sorptive cells. In cancer cells the distribution of CEA lost its polarity completely. CEA was found on the lateral surface or even over the entire surface of cancer cells and in intracellular membranous struc. tures. The biological significance and mechanisms of abnormal distribution of CEA are discussed.     

胃癌癌胚抗原(CEA)的免疫组织化学研究——光镜及免疫电镜观察

本文应用 PAP 法对胃癌 CEA 含量和分布作了光镜和免疫电镜观察,探讨其与胃癌大体类型、组织学类型、生物学行为等的关系及其生物学意义;并结合粘液组织化学方法探讨其在胃癌组织发生研究中的意义。观察研究包括两个部分。第一     

DISTRIBUTION AND ULTRASTRUCTURAL LOCALIZATION OF CARCINOEMBRYONIC ANTIGEN (CEA) IN SIGNET RING CELLS OF GASTRIC CANCER

The distribution and ultrastructural localization of CEA in signet-ring cells of 15 gastric cancer specimens were observed by PAP and immunoelectron microscopic methods. The mechanism of abnormal distribution of CEA in the signet-ring cell and its biological significance are discussed. The results showed that the CEA positive rate in signet-ring cells was 100% with the polarity lost in distribution. Under the light microscope, the CEA stain patterns were of two types: cytoplasmic and membranous types. The former was predominant. Under the electron microscope, most of the CEA was distributed on the cell membrane and cytoplasm. CEA was found in intracellular membranous structure of the cancer cells, especially in protein synthesis and transport organellae (RER, Golgi Complex etc.). The synthesis of CEA in cancer cells increased, yet its elimination was somewhat hampered. The result was that the RER became extended and were full of CEA ( ) material. In the free signet-ring cell, there was a small and short