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自抱茎獐牙菜中分得2个黄酮甙,3个san酮甙,一个san酮甙元和一个三萜酸。经光谱和理化分析并与现有文献对照,确定其结构为:1,5,8-三羟基-3-甲氧基san酮-5-O-β-D-吡喃葡萄糖苷(I)、4',5-二羟基-7-甲氧基黄酮-6-C-β-D-吡喃葡萄甙(Ⅳ)、3',4',5,7-四羟基黄酮6-C-β-D-吡喃葡萄糖甙(V)、1,3,6,7-四羟基san酮-2-C-β-D-吡喃葡萄糖甙(Ⅵ) 相似文献
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目的 对盐酸川芎嗪在冻干制备工艺中的稳定性进行考察,为保障含有该成分的其他冻干粉针剂产品的质量提供依据。方法 采用HPLC法测定盐酸川芎嗪的量。色谱条件:流动相为甲醇-水(45:55),体积流量为1.0 mL/min,柱温25℃,进样量10 μL,检测波长为295 nm。检测盐酸川芎嗪在升华干燥0、12、24、48 h后的含量变化。结果 盐酸川芎嗪在10.5~168 μg/mL内呈良好的线性关系,回归方程为Y=25.837X+10.669(R2=1)。此方法检测盐酸川芎嗪在升华干燥12 h后含量减少9%,24 h其含量减少40%以上,48 h几乎完全损失。结论 盐酸川芎嗪在冷冻干燥过程中随时间延长会因升华而使含量损失加重。 相似文献
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目的:通过对同一种复方中药采用水煎和乙醇提取两种不同方法制备样品进行血中吸收成分的比较,为目前中药药代动力学研究所面临的技术难点提供解决方案。方法:采用液一液萃取净化、996-二极管阵列检测高效液相色谱法测定血浆样品。结果:以多种剂量灌胃给予比格犬IBS复方水煎剂,血浆样品中均未检测到任何吸收入血的成分,但是用同样的方法给予比格犬IBS复方乙醇提取样品,在剂量较水提样品低约27倍时,却至少可以检测到20种化学成分。结论:比格犬灌胃给予IBS中药复方乙醇提取样品和给予水提取样品相比,两者吸收入血成分具有重大差异,这一差异对揭示目前中药复方药代动力学研究难点以及提高中药的临床疗效可能潜在重要意义。 相似文献
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目的:探讨山楂叶原花青素多聚体(PPC)对血管内皮细胞钙浓度的影响及其机理,以期阐明其血管活性的机制。方法:体外培养人脐静脉内皮细胞(HUVECs)。以Fura-2标记细胞内钙离子,活细胞工作站连续测定细胞内钙离子浓度30 min。结果:12.5~50 mg/L PPC可以浓度依赖地升高HUVECs内钙离子浓度,其作用方式与经典血管内皮细胞钙激动剂ATP明显不同。其中25和50 mg/L的PPC效果与正常组比较,差异有统计学显著性(P0.01)。抑制胞内钙释放,对这一作用没有影响;而去除胞外钙离子、使用钠钙交换体抑制剂以及去除胞外钠离子,均可显著抑制,甚至取消PPC的作用。结论:PPC具有显著的血管内皮细胞钙活化作用,这可能是其调节血管内皮细胞功能的机制之一。这一作用可能是通过促进胞内钠内流,激活钠钙交换体的逆向转运,从而引起胞外钙内流而达到的。 相似文献
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Although many population pharmacokinetics (PPK) researches have been conducted on chemical drugs, few have been in the field of Chinese medicine (CM). Each ingredient in CMs possesses different pharmacokinetic characteristics, therefore, it is important to develop methods of PPK studies on them to identify the differences in CM drug safety and efficacy among the population subgroups and to conduct quantitative studies on the determinants of CM drug concentrations. To develop an expert consensus on study design and implementation for PPK of CM, in August 2013, 6 experts in the field of PPK, CMs pharmacology, and statistics discussed problems on the PPK research protocol of CMs, and a consensus was reached. The medicines with toxicity and narrow therapeutic windows and with wide range of target population or with frequent adverse reactions were selected. The compositions with definite therapeutic effects were selected as indices, and specific time points and sample sizes were designed according to standard PPK design methods. Target components were tested through various chromatography methods. Total quantity statistical moment analysis was used to estimate PPK parameters of each component and PPK models reflecting the trend of CMs (which assists in reasonable adjustments on clinical dosage). This consensus specifies the study design and implementation process of PPK. It provides guidance for the following: post-marketing clinical studies, in vivo investigations related to the metabolism in different populations, and development and clinical adjustment of dosages of CMs. 相似文献
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中药标准汤剂是经过标准化工艺制备,可用于评价如汤剂、配方颗粒等用药形式质量的参照物。为研究确定杭白菊标准汤剂质量评价方法及其应用,该实验选用10批杭白菊药材,以标准化工艺参数制备杭白菊标准汤剂。选用性状、相对密度、pH、出膏率、转移率、指纹图谱等参数作为杭白菊标准汤剂质量评价方法。并将建立的杭白菊标准汤剂质量评价方法应用于市售2种菊花配方颗粒的检测中。结果表明杭白菊标准汤剂为浅黄褐色的澄清水溶液,相对密度为1.007~1.011,pH5.37~5.56,出膏率19.93%,平均出膏率23.6%~29.69%;转移率以绿原酸计平均56.2%,木犀草苷平均57.4%,3,5-O-二咖啡酰基奎宁酸平均30.6%。指纹图谱相似度在0.864~0.989。此质量评价方法全面考察了标准汤剂的质量。说明得到的杭白菊标准汤剂质量可靠,有代表性。可用于评价标化其他用药形式。 相似文献