放射性碘标记的抗肺癌单克隆抗体在载瘤裸鼠体内的代谢

放射性碘标记的抗人肺癌单克隆抗体2E3和6DI注入载人肺癌移植瘤裸鼠体内,静脉血放射性-时间曲线符合二室开放模型;血放射性清除半减期为3.4d,表观分布容积636ml/kg;标记抗体在体内的脱碘率为18.6％,脱落的放射性碘绝大部分从尿排出。腹腔给药吸收快,半吸收期2.08h,生物利用度89％。~(125)I标记的单克隆抗体注射后1～3d,放射性主要分布在肿瘤、血、肝、脾中,其次为肺、肾、胃、小肠;此后,血和正常组织的放射性下降,肿瘤中的放射性较高。~(131)I标记的单克隆抗体能使移植瘤清晰显像。     

β-肾上腺能激动剂治疗急性胰腺炎

在许多动物实验中,β-肾上腺能激动剂(下称β-激动剂)抑制组织胺及其它血管活性物质引起的微血管通透性增加.本文研究了2种β-激动剂(异丙肾上腺素和间羟异丁肾上腺素硫酸盐)对猫水肿型和出血型胰腺炎的作用.     

人大细胞肺癌单克隆抗体的制备及其碘化后的免疫活性

为进一步扩大肿瘤放射免疫显像和放射免疫治疗的研究和应用,制备了抗人大细胞肺癌单克隆抗体。用人大细胞肺癌细胞(PLA-801)免疫BALB/c小鼠,取脾细胞与骨髓瘤细胞(Sp2/0)融合,筛选出四株分泌抗体性能稳定的杂交瘤,在BALB/c小鼠中产生含抗体丰富     

高脂血症、妊娠和胰腺炎

妊娠期胰腺炎是一种少见的疾病,饼发症多,胎儿死亡率高。妊娠妇女血甘油三酯明显增加;已经证明,高甘油三酯血症、尤其高乳糜微粒血症与胰腺炎发病有一定关系。本文报告三个妊娠伴严重高甘油三酯血症的病例,并就早期诊断和预防措施进行讨论。例1:24岁,初产妇。剖腹产时见腹腔有稀薄的乳状液,术中血压下降,未探查上腹部。患者血甘油三酯87mM/L,胆固醇21mM/L。血浆超速离心去脂后测淀粉酶为1573单位,诊断暴发型出血性胰腺炎,抢救无效,三天后死亡。尸检报告是出血性胰腺炎。家族调查未见高脂血症病人。例2:33岁,入院时肝脾肿大,轻度贫血。空腹     

抗人肺腺癌单克隆抗体在荷瘤裸鼠体内的分布及肿瘤放射免疫显象

Biodistribution and radioimmunoimaging of monoclonal antibody, 5F11, against human lung adenocarcinoma (LTEP-a2) in tumor-bearing nude mice were studied. The binding rate of radioiodinated 5F11 with LTEP-a2, as determined by in vitro binding assay, was 27.64 +/- 5.01%, while the control was less than 5.0%, suggesting that 5F11 reacted specifically with LTEP-a2. Seventy-two, 96 and 120 hr after injection of 125I-5F11, the percentage of dose taken per gram tumor tissue was 4.84%, 6.29% and 6.60%, higher than those of all normal organs. From 48 to 120 hr on, the ratio of tumor to normal tissue (T/NT) in the liver, spleen, kidney, stomach and intestine was more than 2; T/NT in the lung was 3.1-4.7; T/NT in the blood increased gradually and was 1.87 at 96 after injection. Tumor location index was 4.16, while that of the normal tissue was only 1.21. These results showed that human lung adenocarcinoma xenografts in nude mice were specifically located by radioiodinated 5F11. Radioimmunoimaging was performed in tumor-bearing nude mice. The tumors were clearly visualized 72 hr after injection of 131I-5F11. Radioactivity was higher in tumor region than in other regions. The optimal imaging time was 72-96 hr after injection of radioiodinated 5F11.     

放射性碘标记单克隆抗体的研究进展

近几年来,用放射性碘标记单克隆抗体的研究进展很快。本文着重介绍碘化单克隆抗体的优缺点,碘化技术的改进,以及碘化产品的生物活性评价。     

国内放射性核素肿瘤阳性显像的进展

利用亲肿瘤放射性药物进行肿瘤阳性显像能定位诊断肿瘤,增大剂量还可能达到治疗目的,因此多年来一直是国内外重点研究的课题。国内近几年在这方面作了大量研究工作,并取得了一些可喜的进展,现就有关研究动向报告如下 一、亲肿瘤放射性核素显像 除能被具有功能的甲状腺癌细胞摄取外,直接用于肿瘤阳性显像的放射性核素有~(67)Ga、~(111)In、     

蛙皮素单克隆抗体的制备

蛙皮素(Bombesin,MW 1620)是癌细胞自分泌生长因子之一。为肽类半抗原。在交联剂羰二亚胺(ECDI)作用下与牛血清白蛋白(BSA)偶联。克分子比例为:Bombesin:BSA:ECII=60:1:6000。在4℃生理盐水中搅拌、反应8小时后透析,去除羰二亚胺后浓