Effects of Glucocorticoid on Electrical Activities of Hippocampal Neurons in vitro

Intracellular recording of the rat hippocampal slices was established and usedto analyse the effects of glucocorticoid on resting membrane potential as well as inputresistance of the cells in CA1 and CA2 of hippocampal slices.The results indicate thatwithin 1-2 min the steroid hyperpolarized some of the hippocampal neurons with atendency of increasing the cells' input resistance,suggesting that glucocorticoid caninfluence the function of central neurons in a non-genomic pattern,and the underlyingmechanism involves the change of resting membrane potential.     

糖皮质激素对大鼠离体海马神经元电活动的影响

为了分析糖皮质激素对神经系统快速作用的机制,我们首先建立了大鼠海马脑片细胞内记录的技术,然后观察糖皮质激素对海马CA_1、CA_2区神经元细胞膜电位和输入阻抗的影响。实验结果表明;糖皮质激素灌流1～2min即可使一部分海马神经元超极化,同时伴有输入阻抗升高的趋势。这些现象提示:糖皮质激素可以非基因形式作用于细胞膜,作用机制可能在于其改变了膜静息电位。