猪胰细胞膜缩胆囊肽结合蛋白的纯化

从猪胰细胞膜提取、纯化得到一种缩胆囊肽（ＣＣＫ）结合蛋白，达电泳纯。经十二烷基硫酸钠聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ），其亚基分子量为４３．１×１０－２２ｋｇ。此结合蛋白与不同的ＣＣＫ－Ｓｅｐｈａｒｏｓｅ作用时显示，其与硫酸化ＣＣＫ－８、ＣＣＫ－８、硫酸化亮氨酸脑啡肽（Ｌｅｕ－ｅｎｋ）结合活性较大，而与ＣＣＫ－６、ＣＣＫ－４及Ｌｅｕ－ｅｎｋ结合活性较弱。说明ＣＣＫ羧基末端第七位上酪氨酸的硫酸化对ＣＣＫ结合活性有较大影响。并与ＣＣＫ肽链的长度有关。     

猪胰细胞膜缩胆囊肽结合蛋白的纯化

从猪胰细胞膜提取、纯化得到一种缩胆囊肽（ＣＣＫ）结合蛋白，达电泳纯。经十二烷基硫酸钠聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ），其亚基分子量为４３．１×１０＾－２２ｋｇ。此结合蛋白与不同的ＣＣＫ－Ｓｅｐｈａｒｏｓｅ作用时显示，其与硫酸化ＣＣＫ－８、ＣＣＫ－８、硫酸化亮氨酸脑啡肽（Ｌｅｕ－ｅｎｋ）结合活性较大，而与ＣＣＫ－６、ＣＣＫ－４及Ｌｅｕ－ｅｎｋ结合活性较弱。说明ＣＣＫ羧基末端第七位上酪氨酸的硫酸     

福尔马林致痛及针刺时大鼠中央灰质内c－fos的表达及其与前脑啡肽原mRNA的共存

福尔马林致痛及针刺时大鼠中央灰质内ｃ－ｆｏｓ的表达及其与前脑啡肽原ｍＲＮＡ的共存王妙珍，俞逸红，张菁，何莲芳（医学神经生物学国家重点实验室，上海医科大学基础医学院神经生物学教研室２０００３２）本室以往的工作表明伤害性刺激可引起大鼠中脑导水管周围灰质（...     

TEMPORAL ALTERATIONS OF PROOPIOMELANOCORTIN mRNA LEVEL IN RAT HYPOTHALAMIC ARCUATE NUCLEUS FOLLOWING ELECTROACUPUNCTURE

Alterations of proopiomelanocortin (POMC) rnRNA level in the hypothalamic arcuatenucleus were studied in rats grouped under 6h, 24h l 72h, 96h and 96h (EAX2 ) after elec-troacupuncture(EA) using rnolecular hybridization techniques. It was found that after EA, the levelof POMC mRNA rose significantly (P<0.001 ), a maximun being in the 72h group and declining inthe 96h group. In the 96h (EAX2 ) group, where EA was given at the beginning of the 72nd hour after the first one, instead of declining, the mRNA leve1 rose again. The findings may provide an expla-nation for the long postueffect of EA and its accumulation.