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Meksem K Njiti VN Banz WJ Iqbal MJ Kassem MM Hyten DL Yuang J Winters TA Lightfoot DA 《Journal of biomedicine & biotechnology》2001,1(1):38-44
Soy products contain isoflavones (genistein, daidzein, andglycitein) that display biological effects when ingested byhumans and animals, these effects are species, dose and agedependent. Therefore, the content and quality of isoflavones insoybeans is a key to their biological effect. Our objective wasto identify loci that underlie isoflavone content in soybeanseeds. The study involved 100 recombinant inbred lines (RIL) fromthe cross of ‘Essex' by ‘Forrest,' two cultivars that contrastfor isoflavone content. Isoflavone content of seeds from each RILwas determined by high performance liquid chromatography (HPLC).The distribution of isoflavone content was continuous andunimodal. The heritability estimates on a line mean basis were79% for daidzein, 22% for genistein, and 88% for glycitein.Isoflavone content of soybean seeds was compared against 150polymorphic DNA markers in a one-way analysis of variance. Fourgenomic regions were found to be significantly associated withthe isoflavone content of soybean seeds across both locations andyears. Molecular linkage group B1 contained a major QTLunderlying glycitein content (P = 0.0001, R2 = 50.2%), linkagegroup N contained a QTL for glycitein (P = 0.0033, R2 = 11.1%)and a QTL for daidzein (P = 0.0023, R2 = 10.3%) and linkagegroup A1 contained a QTL for daidzein (P = 0.0081, R2 = 9.6%).Selection for these chromosomal regions in a marker assistedselection program will allow for the manipulation of amounts andprofiles of isoflavones (genistein, daidzein, and glycitein)content of soybean seeds. In addition, tightly linked markers canbe used in map based cloning of genes associated withisoflavone content. 相似文献
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Junkins EP Knight S Lightfoot AC Cazier CF Dean JM Corneli HM 《The Journal of school health》1999,69(10):409-412
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Dania Genobile Joanna Gaston Graham F Tallis Joy E Gregory Julia M Griffith Mary Valcanis Diane Lightfoot John A Marshall 《Communicable diseases intelligence》2004,28(2):225-229
Outbreaks of shigellosis in child care are not commonly reported in Australia, however Shigella bacteria can easily spread in these settings. We report an outbreak of shigellosis in a child care centre and discuss the control measures implemented. This investigation identified 20 confirmed cases of Shigella sonnei biotype g and a further 47 probable cases in children and staff who attended a child care centre, and their household contacts. The investigation highlighted the importance of stringent control measures and protocols for dealing with outbreaks of Shigella and other enteric infections in the child care setting, and the importance of prompt notification by both doctors and child care centres, of suspected outbreaks. 相似文献
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Guy Lightfoot 《International journal of audiology》2017,56(8):612-616
Objective: To investigate the possibility that a sloping baseline in an ABR recording has its origins in cardiac activity and if so, identify how it is expressed. Design: The effect of ECG removal on the averaged ABR was investigated at two artefact rejection levels. Study sample: Ten 1-minute records of raw EEG containing ABR responses but contaminated with cardiac activity were recorded from babies under 12 weeks of age and re-averaged using two artefact rejection levels. The slope of the ABR recording was measured. The measurements were repeated after removing effectively the cardiac activity from the records. Results: A sloping baseline was observed at one or both artefact rejection levels in all records. The slope varied as the artefact rejection level was changed, suggesting this may be implicated in slope generation. The slope effectively disappeared when the cardiac activity was removed from the record. Conclusions: Cardiac activity has the potential to cause a sloping ABR baseline. A possible explanation for this effect is offered, together with suggestions for tester strategy when a sloping ABR baseline is seen in a clinical setting. 相似文献
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Stoner Marie C. D. Bhushan Nivedita L. Maragh-Bass Allysha C. Mitchell John T. Riggins Linda Walker Maleka LeMasters Katherine Reese Bianka Dearing Aissa Debnam Summer Golin Carol Lightfoot Alexandra F. Pettifor Audrey 《AIDS and behavior》2022,26(5):1672-1683
AIDS and Behavior - Adolescents and young adults (AYA; 13–24 years-old) comprise 22% of new HIV infections in the United States (US), most of whom live in the South. We used the... 相似文献
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A case of endocarditis due to Erwinia herbicola is reported. Three years previously the patient had been fitted with a porcine xenograft. 相似文献
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George V. Vahouny PhD Subramaniam Satchithanandam MS Fred Lightfoot BS Lauretta Grau MS Sally Haas-Smith BS David Kritchevsky PhD Marie M. Cassidy PhD 《Digestive diseases and sciences》1984,29(5):432-442
In order to assess the effects of free or resin-bound bile acids on colonic topography, adult rats were surgically provided with an indwelling infusion catheter in the proximal cecum, which exited at the neck behind the head. Conscious, unrestrained rats were allowed chowad libitum and were administered 1 ml of an infusion mixture twice daily for five days. The infusion mixtures included either carrier saline, 100 mg cholestyramine, 165 μmol mixed bile acids, or the bile acids bound to cholestyramine. Additional groups of rats were fed defined diets with and without 2% cholestyramine. Compared to fed controls, colonic infusions of saline had little effect on colon topography. Infusions of 100 mg of cholestyramine in saline twice each day did cause some apparent damage to surface morphology of the colon, but not to the extent observed during feeding of the resin as 2% of the diet. In contrast, extensive surface damage of the colon was observed by twice daily infusions of either 165 μmol of an equimolar mixture of cholic, deoxycholic, and chenodeoxycholic acids, or by the bile acids mixed previously with the ion-exchange resin. The data suggest that topographical damage of the colon observed during feeding of bile acid-sequestering resins is in large part due to increased concentrations of either bound or unbound bile acids in the large bowel. 相似文献