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Homologous testis transplantation in dogs 总被引:4,自引:0,他引:4
Evert J. Barten Hayrabet Garybian Pieter J. Klopper Donald W. W. Newling 《Transplant international》1997,10(5):362-368
There is growing interest in the possible use of homologous testis transplantation for the treatment of anorchia and male
infertility. In order to test the surgical and immunological feasibility of this therapy, three series of experimental studies
of homologous testis transplantation were carried out in dogs. In the first pilot study, four beagles from the same litter
were transplanted using microsurgical techniques for end-to-end anastomosis of the testicular vessels and the vas deferens.
These dogs received cyclosporin A (CyA) for 3 months after transplantation. The longest functional graft survival in this
series was 163 days, strongly suggesting that long-term survival of a homologously transplanted testis graft is possible.
A second series of operations was performed on ten mongrel dogs. The same surgical technique was employed and the series was
divided into three groups.
Group 1 received CyA monotherapy, group 2 a combination of CyA and prednisolone, and group 3 received no immunosuppression.
The average graft survival time in this series was 18 days, significantly less than the 71 days in the first series. The dogs
in group 2, however, had graft survival times that were three times longer than those in the other two groups, suggesting
that CyA in combination with prednisolone yields the best graft survival. In the third series, five littermates received a
testis graft after castration. Immunosuppression was achieved by administration of CyA and prednisolone for 3 months. In three
out of five animals, the graft survived until the immunosuppressive therapy was suspended. Histological biopsies of the graft
3 months after transplantation showed the same maturation of sperm cells as in the control testis of the same dog. The results
of the last series suggest that long-term survival of homologously transplanted testis grafts in dogs is, indeed, possible
with the aid of CyA and prednisolone.
Received: 14 August 1996 Received after revision: 21 March 1997 Accepted: 24 April 1997 相似文献
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