世界首报染色体异常核型的遗传效应分析

目的探讨世界首报染色体异常核型产生的遗传效应.方法外周血染色体常规分析.结果1例不孕合并子宫肌瘤异常核型为46,XX,t(10;16)(q11;p13);1例为不良产和自然流产为46,XX,t(7;11)(q21;q24);另1例仅表现为自然流产为46,XX,t(2;8)(p25;q22).结论3例异常核型为世界首报,由此产生的遗传效应分别导致不孕、不良产、自然流产.     

兰州地区464例遗传咨询病人的染色体分析

目的探讨染色体异常在遗传咨询病人中的发生情况.方法取受检者外周血进行淋巴细胞培养,常规收获制片,G显带处理,显微镜下进行核型分析.结果在464例病人中共检出染色体异常24例,染色体异常检出率为5.17%,其中平衡易位3例(12.5%),罗伯逊易位3例(12.5%),倒位5例(20.83%),性染色体异常8例(33.33%),染色体不平衡易位5例(20.83%).结论妇产科及生殖内分泌许多疾病与染色体异常有关,对这些病人进行染色体检查很有必要.     

Protective effect of intense pulsed light on fibroblast injury induced by UVA Ⅰ

Objective To study the protective effects of intense pulse light (IPL) on the injury of normal human skin fibroblasts (FB) induced by ultraviolet A (UVA Ⅰ ) in vitro and to explore its possible mechanism. Methods The human skin fibroblasts were isolated and cultured, and then irradiated by UVA Ⅰ (9 J/cm2) and IPL (15 J/cm2), respectively. The proliferative ability of the cells were detected by CCK-8. Cell cycle was detected by flow cytometry, and cylin D1 and CDK2 protein expression levels were detected by Western blot. Results Different doses of UVA Ⅰ irradiation caused certain damages of cultured fibroblasts. With the increasing of of UVA Ⅰ dose, cell proliferation was decreased. Cells went to death at the exposure to 11 J/cm2 UVA Ⅰ , while the proliferative activity did not change much at 7 J/cm2 UVA Ⅰ . Cells were treated with UVA Ⅰ for other 2 days, then with IPL irradiation for other 2days, showing clear stimulating to the cell proliferation as compared with the cells that received UVA Ⅰ treatment only. Flow cytometry results showed that an increase of cell proliferating index, and cell cycle protein cyclin D1 and CDK2 expression levels were also upregulated after IPL irradiation.Conclusion UVA Ⅰ irradiation may cause cell damage as showed by cell growth index, cyclin D1 and CDK2 expression, and this injury could be protected partly by IPL treatment. The intense pulsed light may regulate the expression of cyclin proteins that may promote normal fibroblast proliferation, which could be one of the mechanisms of IPL skin rejuvenation.     

重症植物日光性皮炎1例

<正>1病历摘要患者女,52岁。因面部、颈部及双手背肿胀,瘀斑伴疼痛3d,于2017年5月15日收入我院皮肤科。患者3 d前进食大量灰菜并暴晒30 min后双手背、面部和颈部出现肿胀,伴有阵发性刺痛、瘙痒,后双眼睑出现高度肿胀,不能睁眼。先后就诊于当地两家医院,均诊断为     

46,XY,t(6;15)伴习惯性流产及死胎一例

患者男 ,2 9岁 ,结婚 5年。妻子妊娠 4次 ,在无任何诱因的情况下 ,前 3次于孕 50天～ 60天时发生自然流产 ,第 4次在妊娠 7 月时胎儿死于宫内。孕期均无病毒、细菌感染史。无服药、有害物质及Ｘ线接触史。夫妇非近亲婚配。患者表型、智力正常。精液检查也未见异常。妻子 2 5岁 ,身体健康 ,妇科检查正常。双方务农 ,家族中无类似病史。夫妇双方外周血淋巴细胞培养 ,常规制片 ,Ｇ显带。患者核型为 4 6,ＸＹ ,ｔ( 6;15) ( 6ｐｔｅｒ→ 6ｑ 2 5::15ｐ12→ 15ｐｔｅｒ;15ｑｔｅｒ→ 15ｐ 12 ::6ｑ 2 5→ 6ｑｔｅｒ)妻子核型4 6,ＸＸ。讨论　染色…     

多次强脉冲光照射对皮肤成纤维细胞老化指标的影响

目的 探讨多次强脉冲光(intense pulsed light,IPL)照射对皮肤成纤维细胞老化指标的影响,并用长波紫外线(ultraviolet A,UVA)作对比,观察多次强脉冲光照射能否造成细胞老化.方法 实验分3组,一组朱接受照射作为对照组,其他两组分别接受UVA 9 J/cm2和IPL 15 J/cm2照射,每天1次,连续照射5d.在第6天,收集细胞,分别进行β半乳糖苷酶(SA-β-Gal)染色及细胞周期、细胞内活性氧和端粒长度的测定.结果 连续5 d IPL照射后,SA-β-Gal染色和端粒长度与正常对照组相比,未见明显变化(P＞0.05).细胞周期中G1期所占百分比下降,细胞内活性氧下降(P＜0.05);连续5 d UVA照射后,与正常对照组相比,SA-β-Gal染色阳性细胞的百分数增加,细胞周期中G1期所占百分比未见明显变化,细胞内活性氧上升,端粒长度缩短(P＜0.05).结论 多次UVA照射可诱导细胞老化,但多次IPL照射不会出现细胞老化.     

Protective effect of intense pulsed light on fibroblast injury induced by UVA Ⅰ

Objective To study the protective effects of intense pulse light (IPL) on the injury of normal human skin fibroblasts (FB) induced by ultraviolet A (UVA Ⅰ ) in vitro and to explore its possible mechanism. Methods The human skin fibroblasts were isolated and cultured, and then irradiated by UVA Ⅰ (9 J/cm2) and IPL (15 J/cm2), respectively. The proliferative ability of the cells were detected by CCK-8. Cell cycle was detected by flow cytometry, and cylin D1 and CDK2 protein expression levels were detected by Western blot. Results Different doses of UVA Ⅰ irradiation caused certain damages of cultured fibroblasts. With the increasing of of UVA Ⅰ dose, cell proliferation was decreased. Cells went to death at the exposure to 11 J/cm2 UVA Ⅰ , while the proliferative activity did not change much at 7 J/cm2 UVA Ⅰ . Cells were treated with UVA Ⅰ for other 2 days, then with IPL irradiation for other 2days, showing clear stimulating to the cell proliferation as compared with the cells that received UVA Ⅰ treatment only. Flow cytometry results showed that an increase of cell proliferating index, and cell cycle protein cyclin D1 and CDK2 expression levels were also upregulated after IPL irradiation.Conclusion UVA Ⅰ irradiation may cause cell damage as showed by cell growth index, cyclin D1 and CDK2 expression, and this injury could be protected partly by IPL treatment. The intense pulsed light may regulate the expression of cyclin proteins that may promote normal fibroblast proliferation, which could be one of the mechanisms of IPL skin rejuvenation.     

加味玉屏风散联合穴位敷贴治疗鼻炎的临床研究

〔摘 要〕 目的：探讨采用加味玉屏风散联合穴位敷贴法对鼻炎患者进行治疗的临床效果。方法：选取广州市增城区妇 幼保健院 2020 年 2 月至 2020 年 12 月期间收治的 100 例鼻炎患者，采用随机数字表法将其分为对照组与观察组，各 50 例。 其中对照组实施常规治疗，观察组实施加味玉屏风散联合穴位敷贴治疗。比较两组患者治疗总有效率、复发率、鼻炎症状 与生活质量评分。结果：观察组患者治疗总有效率为 94.0 %，高于对照组的 80.0 %，差异具有统计学意义（P ＜ 0.05）； 观察组患者复发率为 30.0 %，低于对照组的 54.0 %，差异具有统计学意义（P ＜ 0.05）；治疗前两组患者的鼻炎症状评分 与鼻腔鼻窦结局测试 22 条（SNOT–22）评分比较，差异无统计学意义（P ＞ 0.05），治疗后两组患者的鼻炎症状评分与 SNOT–22 评分均有不同程度改善，且观察组优于对照组，差异具有统计学意义（P ＜ 0.05）。结论：对于鼻炎患者而言， 对其实施加味玉屏风散联合穴位敷贴治疗，可获得显著的临床疗效，其复发率较低，同时可有效改善其鼻炎症状，从整体 上提升其生活质量。     

浅谈医务人员心理和谐的重要意义和实现途径

医务人员心理和谐问题,是现代社会对医务人员整体素质和综合素质提出的新要求,也是建设和谐社会,促进和谐医院发展的要求.本文阐述了医务人员心理和谐的意义.影响因素以及如何实现心理和谐的途径.     

强脉冲光对长波紫外线Ⅰ诱导成纤维细胞光损伤的保护作用

目的 研究强脉冲光（intense pulse light,IPL）对长波紫外线（ultraviolet A,UVA Ⅰ）诱导的正常离体人皮肤成纤维细胞（fibroblast,FB）损伤的保护作用,探讨以IPL为手段的光子嫩肤技术的理论基础.方法 分离并培养人FB,用不同剂量UVA Ⅰ照射FB以诱导细胞光损伤,CCK-8检测其增殖能力的情况,根据预实验结果确定IPL剂量进行照射,流式细胞仪技术枪测细胞周期,Western印迹法检测CylinD1和CDK2蛋白的表达水平.结果 不同剂量的UVA Ⅰ可造成FB的损伤,随着UVA Ⅰ剂量的增加,细胞增殖能力下降,11 J/cm2的剂量可明显导致细胞大量死亡,而7 J/cm2的UVAⅠ对细胞的增殖能力没有明显的影响;经UVA Ⅰ照射2 d后再进行IPL连续照射2 d,细胞增殖活性高于单独UVA Ⅰ处理组,差异具有统计学意义.流式细胞仪检测结果表明该组细胞增殖指数升高.细胞周期蛋白CyclinD1和CDK2的表达水平升高.结论 IPL可通过调节周期蛋白的表达而促进正常FB增殖,从而保护UVAⅠ诱导的FB光损伤,为应用IPL面部美容除皱即光子嫩肤术提供理论依据.