神经细胞分泌因子促进骨骼肌细胞生长

BACKGROUND: There is no effective treatment for muscle atrophy caused by peripheral nerve damage. Skeletal muscle cells, a structural unit of muscle contraction, can be used for studies on muscle atrophy when cultured in vitro. OBJECTIVE: To investigate the promotion effect of neuron-secreted factors on the growth of skeletal muscle cells in vitro. METHODS: Skeletal muscle cells primary cultured in vitro were divided into two groups: experimental group with neuron-secreted factors, and control group with common culture medium, respectively. Afterwards, the number of skeletal muscle cells and expression level of alpha actin were detected by hematoxylin-eosin staining and immunohistochemical staining, respectively. RESULTS AND CONCLUSION:The number of skeletal muscle cells and expression level of alpha actin in the experimental group were significantly higher than those in the control group (P < 0.05). In conclusion, neuron-secreted factors have the ability of promoting the growth of skeletal muscle cells and may be helpful for denervated muscle atrophy. Subject headings: Tissue Engineering; Satellite Cells, Skeletal Muscle; Actinin; Neurons; Cells, Cultured 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

ADRB2基因调控区多态性与儿童哮喘易感性的相关性研究

目的 研究β2肾上腺素受体（β₂-drenergic receptor,ADRB2）基因调控区rs11168070、rs17108803、rs2053044、rs12654778、rs11959427和rs2895795位点单核苷酸多态性（single nucleotide polymorphism,SNP）/单倍型与儿童哮喘的关系,从而进一步探索儿童哮喘的发病机制。 方法 前瞻性选取2016年10月至2020年10月就诊的哮喘儿童143例（哮喘组）,其中轻度61例（轻度组）,中重度82例（中重度组）;137例健康儿童作为对照组。采集两组儿童外周静脉血,应用SNaPshot SNP技术检测所有儿童ADRB2基因调控区rs11168070、rs17108803、rs2053044、rs12654778、rs11959427和rs2895795位点SNP并分析单倍型,比较哮喘组和对照组儿童ADRB2基因调控区6个位点SNP/单倍型与哮喘易感及病情程度的关系。 结果 哮喘组和对照组儿童ADRB2基因调控区6个位点存在多态性,其中rs2895795（-1429T/A）、rs2053044（-1023G/A）和rs12654778（-654G/A）位点基因型及等位基因频率分布差异有统计学意义（P<0.05）。ADRB2基因调控区6个位点SNP存在连锁不平衡,构成的单倍型TATGCT、TATGGC和AGTGCT与儿童哮喘易感相关,其中TATGCT和TATGGC是儿童哮喘的危险因素（分别OR=1.792、1.946,P<0.05）,而AGTGCT是保护性因素（OR=0.523,P<0.05）。 结论 ADRB2基因调控区SNP/单倍型与儿童哮喘易感性相关,其构成的单倍型TATGCT和TATGGC是儿童哮喘的危险因素,AGTGCT是保护性因素。 引用格式: