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排序方式: 共有820条查询结果,搜索用时 62 毫秒
1.
Shunsuke Iriyama Haruyo Yamanishi Naomi Kunizawa Tetsuji Hirao Satoshi Amano 《Experimental dermatology》2019,28(3):247-253
Daily exposure to sunlight is known to affect the structure and function of the epidermal basement membrane (BM), as well as epidermal differentiation and epidermal barrier function. The aim of this study is to clarify whether the inhibition of BM‐degrading enzymes such as heparanase and matrix metalloproteinase 9 (MMP‐9) can improve the epidermal barrier function of facial skin, which is exposed to the sun on a daily basis. 1‐(2‐hydroxyethyl)‐2‐imidazolidinone (HEI) was synthesized as an inhibitor of both heparanase and MMP‐9. HEI inhibited not only the BM damage at the DEJ but also epidermal proliferation, differentiation, water contents and transepidermal water loss abnormalities resulting from ultraviolet B (UVB). This was determined in this study by the use of UVB‐induced human cultured skins as compared with the control without HEI. Moreover, topical application of HEI improved epidermal barrier function by increasing water content and decreasing transepidermal water loss in daily sun‐exposed facial skin as compared with non‐treated skins. These results suggest that the inhibition of both heparanase and MMP‐9 is an effective way to care for regularly sun‐exposed facial skin by protecting the BM from damage. 相似文献
2.
3.
Takashi Ito Ryuji Sakakibara MD Tatsuya Yamamoto Tomoyuki Uchiyama Zhi Liu Masato Asahina Morihiro Higashi Kimihito Arai Shoichi Ito Yusuke Awa Kaori Yamamoto Mika Kinou Tomonori Yamanishi Takamichi Hattori 《Clinical autonomic research》2006,16(1):66-71
Abstract Uro-neurological assessment was performed in four patients with small-fiber neuropathy due to amyloidosis (2 transthyretin-type/2
immunoglobulin light-chain-type). Voiding difficulties were due to detrusor weakness and impaired bladder sensation. In two
patients cholinesterase inhibition treatment caused urge incontinence, indicating detrusor denervation supersensitivity. The
underlying mechanisms of urinary dysfunction seem to involve postganglionic cholinergic and afferent somatic nerves. 相似文献
4.
5.
Development of inactivated vaccine against virus causing haemorrhagic fever with renal syndrome 总被引:2,自引:0,他引:2
K Yamanishi O Tanishita M Tamura H Asada K Kondo M Takagi I Yoshida T Konobe K Fukai 《Vaccine》1988,6(3):278-282
B-1 virus belonging to the hantavirus group was serially passaged in the brains of newborn mice. Inactivated vaccine was prepared from the brains after inactivation with formalin and then purification by ultracentrifugation. The antigenic potency of this vaccine in vitro was determined by antibody-bound enzyme-linked immunosorbent assay (ELISA) and serial diluted vaccine bound to an aluminium hydroxide gel was inoculated into Balb/c mice to test immunogenicity. After two injections of this vaccine preparation, antibodies were detected in the mice by immunofluorescent, neutralizing and haemagglutination inhibition antibody tests. When mice immunized with this vaccine were challenged with B-1 virus and Hantaan virus (KHF-83-61BL strain), the virus titres in their lungs and spleens were significantly less than those in non-immunized mice. These results suggest that inactivated B-1 virus vaccine is effective against virus challenge by homotypic (B-1 virus) and heterotypic (Hantaan virus) viruses. 相似文献
6.
Mr N. Ishikawa S. Suda T. Sasaki T. Yamanishi H. Hosaka K. Yasuda H. Ito 《Medical & biological engineering & computing》1998,36(6):704-710
A system composed of a functional continuous magnetic stimulator (FCMS) and a saddle-type coil has been developed for non-invasive
treatment of urinary incontinence, especially stress incontinence and urge incontinence. The FCMS conditions were as follows:
2 kW maximum electrical power consumption, 800 V maximum capacitor voltage, 720 μs pulsewidth (180 μs rise time), and 5–30
Hz frequency. A frequency between 5 and 10 Hz is used to treat urge incontinence and a frequency between 25 Hz and 30 Hz is
used to treat urge incontinence. The coil (120 mm long, 90 mm wide and 50 mm thick) fits the most suitable region for this
treatment, the region from the anus to the perineum. The coil is cooled to maintain a coil temperature between 20 and 25°C
so that it can be used efficiently and safely. In experiments with anaesthetised dogs, it was confirmed that the urethral
pressure increased when the circumference of the perineum received continuous magnetic stimulation of 720 μs pulsewidth (180
μs rise time), 10Hz frequency and about 520 V capacitor voltage. This result suggests that magnetic stimulation can be effective
as a urinary incontinence therapy. 相似文献
7.
Acyclovir (9-/2-hydroxyethoxymethyl/guanine) enhanced the plaque formation of HSV and VZV at subinhibitory dose and inhibited at higher concentration but not in thymidine kinase deficient viruses. The enhancement was neutralized by thymidine. Induction of viral thymidine kinase activity was not affected with ACV. These results suggest that the enhancement may be mediated by viral thymidine kinase. 相似文献
8.
K. Shiraki M. Ishibashi T. Okuno J. Namazue K. Yamanishi T. Sonoda M. Takahashi 《Archives of virology》1991,117(3-4):165-171
Summary Azathioprine (Aza) was found to have anti-human cytomegalovirus (HCMV) activity in vitro at concentrations used for immunosuppression therapy. The dose of Aza for 50% plaque reduction was 0.592µg/ml for HCMV in human embryonic lung (HEL) cells, but those of Aza for 50% plaque reduction for herpes simplex virus (HSV) and varicella-zoster virus were more than 20µg/ml. The dose of Aza for 50% reduction of the HCMV yield in infected cells was 0.25µg/ml, while that for 50% reduction of the HSV yield in infected cells was more than 50µg/ml. The dose of Aza for 50% growth inhibition of HEL cells was 30µg/ml, and 50.7 and 120 times greater than the doses for 50% reduction of the plaque formation and the yield of HCMV, respectively. Thus Aza was found to have a strong anti-HCMV activity at concentrations used for immunosuppression. When HCMV infected cells were treated with cyclosporine (CsA: 0.2µg/ml) and prednisolone (Pred: 0.3µg/ml) simultaneously with Aza, the doses of Aza for 50% reduction of plaque formation and the yield of HCMV were 0.73 and 0.32µg/ml, respectively. Thus an inhibitory effect of Aza was also observed in HCMV-infected cells treated with CsA and Pred at their concentrations used for immunosuppression. Maintenance of an anti-HCMV dose of Aza in combination with CsA and Pred might establish not only satisfactory immunosuppression but also suppression of HCMV infection in transplant recipients. 相似文献
9.
Haruyo Nakajima PhDa Satoshi Hachimuraa Shinya Nishiwakia Toshiyuki Katsuki MD PhDb Naoki Shimojo MD PhDb Akio Ametani PhDa Yoichi Kohno MD PhDb Shuichi Kaminogawa PhDa 《The Journal of allergy and clinical immunology》1996,97(6):1342-1349
To study cow’s milk allergy at the cellular level, we assessed the reactivity of peripheral blood mononuclear cells from patients allergic to cow’s milk to αs1-casein, which is one of the major allergens in cow’s milk. Proliferation of the cells to αs1-casein activation showed a rather weak response. Therefore to understand T-cell reactivity to αs1-casein in more detail, we prepared αs1-casein–specific T-cell lines from patients allergic to cow’s milk and established 26 T-cell lines. These T-cell lines could be classified into three groups by analyzing their surface marker expression: those containing predominantly CD4+CD8- T cells, those containing both CD4+CD8- and CD4-CD8+ T cells, and those containing predominantly CD4-CD8+ T cells. The CD8+ T cells were obtained at an unexpectedly higher frequency from the patients. These T-cell lines produced interferon-γ and IL-4. These results suggest that CD8+ T cells specific for αs1-casein and CD4+ T cells were primed by the stimulation with αs1-casein in patients allergic to milk and that both T cells may play a key role in the onset, progression of, or recovery from cow’s milk allergy. (J ALLERGY CLIN IMMUNOL 1996;97:1342-9.) 相似文献
10.
Toshiomi Okuno Koichi Yamanishi Kimiyasu Shiraki Junko Namazue Michiaki Takahashi 《Virus research》1988,10(4):357-367
HL-60 cells can be induced to differentiate into macrophage-like cells by treatment with 12-O-tetra-decanoylphorbol-13-acetate (TPA). The relationship between virus replication and cell differentiation was investigated using HL-60 cells that had been induced to differentiate by TPA (dHL-60 cells) and undifferentiated cells (udHL-60 cells). On infection of these cells with cell-free varicella-zoster virus (VZV), virus antigens were detected in dHL-60 cells but not in udHL-60 cells, and the percentage of antigen-positive dHL-60 cells increased during incubation. Similar results were obtained by infectious center assay, and the percentage of antigen-positive cells correlated with the stage of cell differentiation. No significant difference was found in the binding of VZV to dHL-60 cells and udHL-60 cells. Furthermore, trypsin treatment after adsorption suggested that VZV penetrated into udHL-60 cells. These findings indicate that VZV may be able to replicate in mature monocytes but may be harbored in immature monocytes in vivo. 相似文献