女童外阴阴道炎32例衣原体和支原体感染临床观察

1998年8月—2002年10月我科收治衣原体和支原体感染所致女童外阴阴道炎32例，现将临床治疗结果报告如下。     

血小板严重减少的系统性红斑狼疮2例

我科收治2例血小板严重减少的系统性红斑狼疮患者,经不同方案治疗后血小板回升,病情明显好转,现报告如下。1临床资料例1.女,21岁。因面部红斑、发热1周于2004年10月入院。体格检查:体温38.9℃。颊黏膜见2个直径约0.5cm的溃疡。双下肢膝以下呈凹陷性水肿。皮肤科检查:颧部见多个类圆形淡红斑,直径1～2cm,边缘欠规则。甲周见红斑和毛细血管扩张,指尖见不规则红斑。实验室检查:血常规中白细胞5.76×109/L,血小板4×109/L(正常值100￣300×109/L,以下同),其后反复查血小板计数均为(1～3)×109/L。C30.44g/L(0.85￣1.93×109/L),C40.10g/L(0.…     

三种常见脱发性疾病与睡眠质量和情绪因素的相关性调查

目的：调查和探讨生理性脱发、雄激素性脱发、斑秃与睡眠质量和情绪因素的相关性。方法采用匹兹堡睡眠质量指数（PSQI）及焦虑自评表（SAS），对我院皮肤科确诊为生理性脱发、雄激素性脱发、斑秃的患者及健康对照者进行调查和分析。结果3种脱发患者与健康对照者在青年组（15～29岁）、壮年组（30～44岁）、中老年组（＞45岁）的PSQI及SAS得分逐渐增加，组间比较差异具有统计学意义（P＜0.01）。斑秃患者PSQI总分＞7分，有睡眠问题；在PSQI的7个指标中，青年组主要在睡眠质量、入睡时间、睡眠效率、睡眠障碍有差异（P＜0.01）；壮年组在睡眠质量、入睡时间、睡眠障碍、日间功能障碍上有差异（P＜0.05）；中老年组在入睡时间、睡眠效率、睡眠障碍上有差异（P＜0.05）。青年组的斑秃及雄激素性脱发患者SAS得分高于国内常模分值。脱发对于女性患者PSQI及SAS积分的影响大于男性。经Pearson积矩相关分析（r=0.487， P=0.000），说明睡眠与情绪焦虑之间存在正相关。结论脱发性疾病，尤其是斑秃同时受到睡眠质量、精神因素的影响，因此恢复良好的生活习惯和调整情绪对预防斑秃的发生有重要价值。     

NSAIDs预防ERCP术后急性胰腺炎的研究进展

目的 内镜逆行胰胆管造影术（ERCP）是肝脏、胆道、胰腺疾病诊断与治疗的重要手段之一.在将ERCP用于临床工作中,也会带来一些并发症,其中ERCP术后并发的急性胰腺炎以其较高的发生率及危险性引起了广大临床医师的重视.为了降低ERCP术后急性胰腺炎的发生率,临床医师在行ERCP术时运用各种手段及药物来减少其发生.非甾体抗炎药（NSAIDs）作为一类廉价、给药方便、不良反应小的药物在预防ERCP术后急性胰腺炎中有着较好的应用前景.     

相关知识缺乏致系统性红斑狼疮患者的安全隐患

报道38例系统性红斑狼疮患者因相关知识乏,而导致的患者的心理障碍、意外外妊娠致狼疮活动、感染、结核感染、消化道出血、激素不规范使用致狼疮活动的安全隐患。     

皮肤免疫病理(四)

第一章免疫荧光技术基础一、荧光(己载《皮肤病防治》85年1—2期)二、荧光色素(已载《皮肤病防治》85年3—4期)第二章免疫荧光技术在皮肤病学中的应用一、方法学(已载《皮肤病防治》86年1—4期)二、皮肤抗原人的皮肤有多种抗原,至少可分三类:①自身抗原(Self—antigens);②改变了的自身抗原(Altered Self—antigens):多由皮肤损伤与非自身抗原相互     

喘可治对小鼠异基因骨髓移植中移植物抗宿主病效应的影响

<正>喘可治注射液(简称CKZ)主要成分为淫羊藿、巴戟天提取物,临床传统用于治疗哮喘。前期研究结果显示,喘可治具有复杂的免疫调节作用,影响T细胞的活化、细胞因子分泌(TH1/TH2平衡)、增殖和凋亡等行为,具有一定双向调节功能,即免疫反应过强时抑制免疫应答,免疫反应过弱时     

Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway

Objective:To study the immunologic function of dendritic cells(DCs) cultured in two kinds of hepatoma cell line’s supernatant and the enhancing effects of carboxymethylpachymaran(CMP) on DCs.Methods:DCs were harvested after stimulation by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin(IL)-4 from umbilical cord blood using density-gradient centrifugation method. Cultured supernatant of two hepatoma cell lines(HepG2 and HepG2.2.15) were collected for condition medium(CM) according to a volume ratio of supernatant to incomplete RPMI-1640 medium,which was 3:1.CMP was dissolved in incomplete RPMI-1640 medium.Experimental groups were divided according to the culture medium,either CM or with CMP in it.DCs subsets CD83,CD86,CD1a,and d-related human leukocyte antigens(HLA-DR) were analyzed by flow cytometry.The proliferation ability of allogeneic T cells in mixed lymphocyte reaction(MLR) stimulated by DCs was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) analysis.IL-12p70,interferon-γ(IFN-γ),and nuclear factor k B(NF- k B) were detected by enzyme-linked immunosorbent assay analysis.Results:The proliferation of lymphocytes and secreting level of IL-12 and expression of phenotype of DCs cultured in two kinds of CM were lower than those of normal group(P<0.01).Compared with the normal group,groups treated with CMP showed a higher expression level of DCs subsets,lymphocyte reproductive activity,as well as IL-12 and IFN-γsecretion levels.Groups treated with CMP also demonstrated higher levels of DCs phenotype expression and IL-12 and IFN-γsecretion in supernatant of MLR and higher lymphocyte reproductive activity compared with CM group(P<0.05).Compared with the normal group,the expression level of NF-k B in DCs nuclear was higher in CMP groups but lower in two CM groups(P<0.05).After CMP was added,the NF-κB expression levels of two CM groups were increased compared with levels before CMP was added(P<0.05).However,there was no significant difference between the two CM groups(P>0.05).Conclusions:Two kinds of hepatoma cell line’s supernatant can inhibit the immunologic function of DCs.This suppressive effect may be related to the inhibition of NF-κB/Rel pathway.CMP may up-regulate the DCs function by activating the NF-κB/Rel pathway.     

系统性红斑狼疮疾病活动性判断探讨

<正> 系统性红斑狼疮(SLE)疾病活动性判断标准一般是根据免疫学指标(血清C_3、CH_50;dsDNA抗体;免疫复合物)及与之相关联的一些临床表现作为指标,按半定量记分法制定的。目前临床上较常使用的标准有根据临床表现判断损害脏器数目(不考虑权数)的一般标准,以及Malcolm的按几个主要临床症状记分的加权法记分标准(简称M标准)。迄今尚无我国人自定的标准。本文根据与SLE免疫     

SLE与妊娠

系统性红斑狼疮(Systemic Lupus Erythematosus,SLE)是典型的自身免疫性疾病,多见于育龄妇女。近四十年来,由于医学技术的进步,SLE的妊娠过程发生了很大变化。五十年代,妊娠常伴随疾病严重恶化、胎儿甚至母亲死亡。但近十多年来,SLE病人妊娠已属平常,后果大为改观。