Increased bonding of a glass-ionomer cement to dentin by means of FeC13

Abstract – It was previously demonstrated by other authors that FeC1₃ is readily sorbed by dentin surfaces. The main purpose of the present work was to investigate the possibility of increasing the bonding of a glass-ionomer cement to dentin by means of FeC1₃. It was found that the strength of the bond was approximately doubled when the dentin was treated with an acidic cleansing agent followed by an application of an aqueous solution of FeC1₃ of suitable concentration.     

The use of DNA markers for carrier detection and prenatal diagnosis of haemophilia A in Egyptian families

Summary. Haemophilia A is the most common inherited X‐linked recessive bleeding disorder. The aim was to investigate the usefulness of two DNA markers in linkage analysis, one intragenic BCL1 affecting restriction site in intron 18, and is detected as restriction fragment length polymorphism (RFLP), and one extragenic variable number of tandem repeat (VNTR) locus DXS52 (St14) to formulate an informative and accurate carrier detection and prenatal diagnosis. The study included 46 families with at least one child affected with haemophilia A, and 30 unrelated normal females as control group. Polymerase chain reaction (PCR) and restriction enzyme analysis were used to study the polymorphism in BCL1, and long‐distance PCR for detection of VNTR (ST14) alleles. The incidence of BCL1 (+) allele was 74%, 72% and 60% in patients, mothers and control group, respectively. Expected heterozygosity for BCL1 was 40% in mothers of affected cases compared with 48% in the female control group. However, observed heterozygosity was found to be 48% in the mothers of affected cases, compared with 60% in the control group. Thus, 48% of the studied families are informative for this marker alone. Nine different alleles of VNTR (St14) were observed in mothers and six alleles in affected cases and six in the control group. The most prevalent alleles were 1300 bp (45.5% and 34%) and 700 bp (13.6% and 20%) in patients and their mothers, respectively. Observed heterozygosity in mothers was 41% compared with 43.3% in controls. The combined use of both BCL1 and St14 markers raised the informative rate to 63.6%. Carrier detection and prenatal diagnosis is possible in haemophilia A families using both DNA markers. We suggest screening haemophilic families first for BCL1 polymorphism followed by analysis of St14 locus.     

A Reappraisal of the Mitral B-Bump (B-Inflection): Its Relationship to Left Ventricular Dysfunction

A prolonged AC interval, decreased PR - AC interval, and a B-inflection ("bump" or "notch") on the mitral AC slope, have been widely regarded as evidence of abnormally high LVEDP for the last 16 years. However, several groups have questioned the reliability of these signs as predictors of high LVEDP. In 50 patients subjected to LV catheterization, we found no correlation between LVEDP and the AC interval or PR - AC interval. A better correlation was obtained between the presence of a B-inflection and diminished LV ejection fraction on angiocardiography. In our series, the B-inflection was noted in 1/19 patients with LVEDP < 15 mmHg as well as LV ejection fraction > 55%, but it was present in 7/10 patients with LVEDP over 15 mmHg, as well as LV ejection fraction < 55%. When properly recorded, the presence of a B-inflection is a useful sign of significant LV dysfunction.     

Stenting versus non-stenting after non-complicated ureteroscopic manipulation of stones in bilharzial ureters

BACKGROUND: Stents were used routinely after ureteroscopy to prevent postoperative ureteral obstruction. However, because of the recognized complications of stents, non-stenting is the new trend after uncomplicated ureteroscopy. The wall of the bilharzial ureter is characteristically thick and may be calcified. The aim of this study is to see if the non-stenting trend could be applied to ureteroscopic manipulation of stones in bilharzial ureters. PATIENTS AND METHODS: In this prospective study, 56 patients, with evidence of bilharzial lesions in the urinary tract, undergoing ureteroscopy for distal ureteral stones were included. After successful uncomplicated stone fragmentation and extraction, patients were randomized into two groups. Group A includes 28 patients in whom double J 6-Fr polyurethane stents were placed for 3 weeks. Group B includes 28 non-stented patients. Postoperative fever, loin pain, lower urinary tract symptoms and change in the degree of hydronephrosis were reported. RESULTS: There was no significant difference in the mean age of patients and stone size in both groups. The mean operative time was 43 +/- 14 min in group A and 38 +/- 11 in group B. There was no significant difference in the mean loin pain score, in the first postoperative 48 h, in both groups (4.4 +/- 0.8 in group A and 4.9 +/- 0.5 in group B). Patients in group A had, significantly, more flank pain with voiding (P < 0.01), voiding pain (P = 0.04), frequency (P = 0.01) and urgency (P = 0.04). Radiologic follow-up was available for 24 patients in group A and 23 patients in group B at the 3-month visit. Hydronephrosis had improved in all patients, in both groups, with no evidence of ureteral stricture. CONCLUSION: Routine placement of stents after uncomplicated ureteroscopy for distal ureteral stones is unnecessary in bilharzial ureters. Moreover, it might be unadvisable because lower urinary tract symptoms and voiding loin pain are more in patients with ureteral stents and hydronephrosis is equally improved in stented and non-stented patients.     

Multilineage mobilization of peripheral blood progenitor cells in humans following administration of PEG-rHuMGDF

The most important physiological regulator of megakaryocytopoiesis is the ligand for the c-mpl receptor (thrombopoietin/megakaryocyte growth and development factor, MGDF). We examined the effect of pegylated-recombinant human MGDF (PEG-rHuMGDF): patients received PEG-rHuMGDF at doses of 0.03, 0.1, 0.3 or 1.0 μg/kg/d or placebo for 10 d maximum in a double-blinded randomized study. There was a dose-dependent elevation in circulating platelet counts but no alteration in erythrocyte or total leucocyte counts. The number of bone marrow megakaryocytes was increased approximately 2-fold. The frequency of bone marrow progenitor cells was not altered. In contrast, both to the bone marrow results and to published pre-clinical data, there was a dose-dependent mobilization into the blood of progenitor cells of multiple cell lineages. Increased levels of Meg-CFC (maximum increase 30-fold), day 7 and day 14 GM-CFC and BFU-E were demonstrated at doses of 0.3 and 1.0 μg/kg/d PEG-rHuMGDF. At 0.1 μg/kg/d, mobilization of Meg-CFC alone occurred in two-thirds of patients. Maximum blood levels of progenitor cells occurred at day 12. Thus, administration of PEG-rHuMGDF to humans resulted in mobilization of progenitor cells of multiple lineages despite its 'lineage-specific' activity on mature cell development.     

ARTHRITIS OF LEPROSY

An inflammatory polyarthritis has been previously describedin leprosy but accounts of the clinical characteristics havevaried. All patients with joint symptoms admitted to a leprosycentre over 5 months were examined by a rheumatologist. Of 48acute admissions, 20 (42%) had a symmetrical polyarthritis affectingthe wrists and fingers. This was strikingly similar to RA inappearance. The arthritis occurred exclusively in patients withreactions mainly during anti-mycobacterial treatment. The clinicalfeatures of the joint disorder were identical in Type I (associatedwith alterations in immune status) and Type II (erythema nodosumleprosum) reactions. Synovial tissue from one patient revealedno mycobacteria. Four of five hand X-rays suggested small erosionsof the finger joints. KEY WORDS: Mycobacterium leprae, Symmetrical polyarthritis, Leprosy reactions     

An audit of abdominal hysterectomy over a decade in a district hospital

Summary. A retrospective survey of abdominal hysterectomy in a district hospital in two separate years (1970 and 1980) was undertaken. The mortality and short-term physical morbidity of the operation was low. Evidence is presented of a change in attitude over the decade by gynaecologists towards abdominal hysterectomy, the trend being increasingly liberal, such that in 1980 nearly half the patients subjected to abdominal hysterectomy had no demonstrable pathology. In view of the reported adverse long-term psychiatric and cardiovascular effects of hysterectomy, even when the ovaries are conserved, this trend must give rise to concern.