Determination of whether morphometric analysis of vertebrae in the domestic cat ( Felis catus ) is related to sex or skull shape

In many mammals, gender and skull shape are related to the bone morphology of the entire body; however, this has not been well established in the domestic     

Cardiopulmonary effects of traditional Thai dance on menopausal women: a randomized controlled trial

[Purpose] This study evaluated the effects of Thai dance on cardiopulmonary factors in menopausal women. [Subjects] Sixty-six menopausal women aged 40 years or more. [Methods] Subjects were randomly assigned to either the Thai dance or control group. The Thai dance group performed a traditional Thai dancing exercise program for 60 minutes, 3 times per week for 6 weeks. The control group received general health guidance. The 6-minutewalk test, peak expiratory flow, forced vital capacity, forced expiratory volume in one second, maximal voluntary ventilation, and chest expansion were assessed at baseline and at the end of the study. [Results] Sixty-six menopausal women were eligible. At the end of the study, all variables were significantly better in the Thai dance group than the control group. Moreover, all variables improved significantly compared to baseline in the Thai dance group but not in the control group. For example, the mean 6-minutewalk test result in Thai dance group at the end of the study was 285.4 m, which was significantly higher than that at baseline (254.8 m) and the control group at baseline (247.0 m). [Conclusion] A 6-week Thai dance program improves cardiorespiratory endurance in menopausal women.Key words: Exercise, Cardiorespiratory endurance, Lung function     

Antibiotic Use in Thailand: Quantifying Impact on Blood Culture Yield and Estimates of Pneumococcal Bacteremia Incidence

No studies have quantified the impact of pre-culture antibiotic use on the recovery of individual blood-borne pathogens or on population-level incidence estimates for Streptococcus pneumoniae. We conducted bloodstream infection surveillance in Thailand during November 2005–June 2008. Pre-culture antibiotic use was assessed by reported use and by serum antimicrobial activity. Of 35,639 patient blood cultures, 27% had reported pre-culture antibiotic use and 24% (of 24,538 tested) had serum antimicrobial activity. Pathogen isolation was half as common in patients with versus without antibiotic use; S. pneumoniae isolation was 4- to 9-fold less common (0.09% versus 0.37% by reported antibiotic use; 0.05% versus 0.45% by serum antimicrobial activity, P < 0.01). Pre-culture antibiotic use by serum antimicrobial activity reduced pneumococcal bacteremia incidence by 32% overall and 39% in children < 5 years of age. Our findings highlight the limitations of culture-based detection methods to estimate invasive pneumococcal disease incidence in settings where pre-culture antibiotic use is common.     

Clinical manifestations and outcome of patients with severe eosinophilic meningoencephalitis presumably caused by Angiostrongylus cantonensis

The clinical manifestations and outcome of patients with severe eosinophilic meningoencephalitis has never been reported. We reported 11 comatose patients with eosinophilic meningoencephalitis. Most of them presented with subacute to chronic headache and fever, followed by acute coma. Cerebrospinal fluid abnormalities were similar to alert patients with eosinophilic meningitis. None of them had received antihelmintic drug and seven patients were treated with corticosteroids. Ten patients died and one patient is still in a coma. Corticosteroids seem to be ineffective in severe eosinophilic meningitis.     

MR imaging findings in cauda equina gnathostomiasis

We report a case of cauda equina syndrome caused by Gnathostoma spinigerum, which was confirmed by an immunoblotting test. MR imaging of the lumbosacral spine showed long, segmented hyperintensity along the cauda equina with irregular enhancement on the postcontrast study. The conus medullaris was slightly enlarged with abnormal enhancement. The patient was treated with corticosteroids, and her clinical condition improved. MR imaging, 9 months after treatment, showed the condition to be completely resolved.     

Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis

Immunoglobulin G subclass antibody (IgG1, IgG2, IgG3, and IgG4) responses to the rat lungworm, Angiostrongylus cantonensis, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for the serodiagnosis of human angiostrongyliasis. Serum samples from patients with proven angiostrongyliasis and from clinically suspected cases of angiostrongyliasis with eosinophilic meningitis were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The results indicate that the immunoblotting used to detect IgG4 antibodies to the antigenic band of an approximate molecular mass of 29 kDa from young adult somatic extract of A. cantonensis is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 89.2%, 75%, 95%, 85.7% and 90.4%, respectively. More importantly, the test can discriminate between human angiostrongyliasis, gnathostomiasis and cysticercosis, three diseases that produce eosinophilic meningitis.     

Two rapid methods of counterstaining flourescent dye tracer containing sections without reducing the flourescence

A method is described for counterstaining neural tissue containing cells that are retrogradely labeled by flourescent dyes or horseradish peroxidase (HRP). Specifically, protocols are detailed for the combined use of the tracers with Methylene blue for a Nissl stain or with silver methods for the detection of acetylcholine esterase. The usefulness of these techniques is evaluated in relation to cortico-cortico and thalamocortico projections. The findings indicate that the methods do not mask the labeling of the most sensitive fluorescent dyes or by HRP. Only the yellow dyes are significantly affected by the Methylene blue counterstain. Further, Fast blue labeling in neurons is not significantly diminished by the Bodian fiber stain. The effect of coverslipping sections containing fluorescent dye labeled cells also was evaluated and found to significantly extend the life of the labeling while not reducing the sensitivity. Thus the two counterstaining techniques provide excellent structural information, do not seriously affect tracer labeling and have few of the disadvantages common to other counterstaining methods.     

Cost of hospitalizing asthma patients in a regional hospital in Thailand

BACKGROUND AND OBJECTIVE: The hospitalization of asthma patients accounts for substantial costs in any health-care system. However, the utilization of medical resources and the cost of hospitalizing asthma patients in Thailand have never been evaluated. This study aimed to evaluate the utilization of health-care resources and itemize the cost of hospitalizing asthma patients in a regional hospital in north-east Thailand. METHODS: A cross-sectional, retrospective study was conducted at Surin Hospital, a 697-bed, regional hospital in north-east Thailand. The study included 183 adults (> or =15 years of age) who were admitted with acute asthma. Demographic data, resource-utilization parameters and itemized costs were recorded. RESULTS: The average hospital cost was 5,809.3 +/- 6,587.4 Thai Baht (US $134.5 +/- 152.5) per patient (range, 740-57,980 Thai Baht or US $17.1-1,342.1). Medications were the primary hospital expense (47.2%), followed by hospital charges (13.5%), nursing care (13.0%) and respiratory therapy (12.2%). Fifteen per cent of patients accounted for 45% of total hospital costs. Significant predictors of increased total hospital cost were: (i) age > or =60 years; (ii) presence of comorbid conditions; (iii) admission to the intensive care unit; and/or (iv) prolonged hospital stay. CONCLUSION: Despite the low unit costs for inpatient care in Thailand, the cost of hospitalizing asthma patients (especially the elderly) constitutes a substantial burden to the health-care system.