血浆凝血因子Ⅶ活性测定的临床意义

血浆凝血因子Ⅶ活性测定的临床意义彭云龙，郭兴城，赵嫣近年来高凝状态在冠心病的发生发展中所起的作用受到了广泛重视，认为冠心病患者处于高凝状态。本文对４０例冠心病患者进行凝血因子Ⅶ活性测定，以探讨其临床诊断意义。１对象与方法对象冠心病组４０例，男３２例，...     

静脉注射心律平终止室上性心动过速致窦性停搏1例

静脉注射心律平终止室上性心动过速致窦性停搏１例辽宁省人民医院（１１００１５）毛文珍，赵晓茹，胡芯茹，顾东红，郭兴城，彭云龙患者男，６５岁。阵发性心悸７年。每次发作用异搏定终止。近１年来发作频繁。入院前心悸１天余。入院后检查：心率１７６次／分，血压１３...     

成人腘窝内骨软骨瘤1例

骨软骨瘤常见于儿童和青少年，部位以四肢长骨干骺端为多见，如股骨下端和胫骨上端。骨软骨瘤临床诊断较容易。我院近期收治1例成人胭窝内骨软骨瘤，现报告如下。     

转化生长因子-β1诱导体外失神经骨骼肌肌源性干细胞分化的研究

目的 探讨转化生长因子-β1(TGF-β1)对体外失神经骨骼肌肌源性干细胞(MDSCs)分化方向的作用.方法 采用差速贴壁法分离出大鼠失神经骨骼肌肌源性干细胞,利用TGF-β1诱导分化.将细胞随机分为两组:A,对照组;B,10 ng/ml TGF-β1处理组.在相差显微镜下观察细胞生长情况,用免疫细胞化学法、RT-PCR和Western blot检测两组细胞中Ⅰ型胶原蛋白(COL-Ⅰ)、Ⅲ型胶原蛋白(COL-Ⅲ)、α-平滑肌肌动蛋白(α-SMA)、波形蛋白(vimentin)和干细胞抗原-1(Sca-1)的表达水平变化.结果 在体外,对照组失神经骨骼肌MDSCs表达和合成COL-Ⅰ、COL-Ⅲ、α-SMA和vimentin的水平极低,而表达和合成Sca-1的水平很高.10 ng/ml TGF-β1处理后,失神经骨骼肌MDSCs能高表达COL-Ⅰ、COL-Ⅲ、α-SMA和vimentin,而低表达Sca-1.在TGF-β1的作用下,失神经骨骼肌MDSCs中COL-Ⅰ的表达水平在第2天时达最高值(12.5591±0.3389),约为对照组的3倍;COL-Ⅲ在5d时达最高值(0.8956±0.0438),约为对照组的23倍;α-SMA在第5天时达最高值(1.1090±0.0018),约为对照组的18倍;vimentin在5d时达最高值(0.1794±0.0019),约为对照组的8.5倍;Sca-1在第2天时开始降低,第5天时降低最显著(0.0636±0.0015).结论 TGF-β1能诱导体外失神经骨骼肌MDSCs向肌成纤维母细胞(myofibroblasts)分化,促进细胞合成和分泌细胞外基质.     

急性下壁心肌梗塞心电图QRS综合液的早期改变及临床意义

急性下壁心肌梗塞心电图ＱＲＳ综合液的早期改变及临床意义辽宁省金秋医院心内科（１１００１５）彭云龙，郭兴城辽宁省人民医院超声心动图室杨冬梅我们对１５例急性下壁心肌梗塞病人胸痛发作后５小时内进行连续心电图监测，以探讨各导联ＱＲＳ综合波的早期变化，现将观察...     

有机磷中毒时特殊情况下阿托品化量的识别方法

在抢救急性有机磷中毒时正确应用阿托品,顺利达化量是提高成活率的关键,但部分病人由于个体受内在和外在因素的影响,部分化量体征不明显,出现假性阿托品化反应,故影响了阿托品在体内与堆积的乙酰胆碱(CH)抗衡作用和超剂量应用阿托品而中毒,最终导致机体重要脏器的严重损害而死亡.     

TGF-β1-induced synthesis of collagen fibers in skeletal muscle-derived stem cells

The aim of this study was to investigate the mechanism of deposition of extracellular matrix induced by TGF-β1 in skeletal muscle-derived stem cells (MDSCs). Rat skeletal MDSCs were obtained by using preplate technique, and divided into four groups: group A (control group), group B (treated with TGF-β1, 10 ng/mL), group C (treated with TGF-β1 and anti-connective tissue growth factor (CTGF), both in 10 ng/mL), and group D (treated with anti-CTGF, 10 ng/mL). The expression of CTGF, collagen type-Ⅰ (COL-Ⅰ) and collagen type-Ⅲ (COL-Ⅲ) in MDSCs was examined by using RT-PCR, Western blot and immunofluorescent stain. It was found that one day after TGF-β1 treatment, the expression of CTGF, COL-Ⅰ and COL-Ⅲ was increased dramatically. CTGF expression reached the peak on the day 2, and then decreased rapidly to a level of control group on the day 5. COL-Ⅰ and COL-Ⅲ mRNA levels were overexpresed on the day 2 and 3 respectively, while their protein expression levels were up-regulated on the day 2 and reached the peak on the day 7. In group C, anti-CTGF could partly suppress the overexpression of COL-I and COL-Ⅲ induced by TGF-β1 one day after adding CTGF antibody. It was concluded that TGF-β1 could induce MDSCs to express CTGF, and promote the production of COL-Ⅰ and COL-Ⅲ. In contrast, CTGF antibody could partially inhibit the effect of TGF-β1 on the MDSCs by reducing the expression of COL-Ⅰ and COL-Ⅲ. Taken together, we demonstrated that TGF-β1-CTGF signaling played a crucial role in MDSCs synthesizing collagen proteins in vitro, which provided theoretical basis for exploring the methods postponing skeletal muscle fibrosis after nerve injury.     

三磷酸腺苷促进肌源性干细胞增殖的作用及机制研究

目的 研究三磷酸腺苷(ATP)促进肌源性干细胞(MDSCs)增殖的作用机制.方法 采用差速贴壁法分离出小鼠MDSCs,将细胞分为两组:实验组(100 μmol/L ATP)和对照组,观察MDSCs的生长情况,用流式细胞仪检测细胞周期,RT-PCR检测MDSCs中ATP受体的表达.结果 ATP作用3d后,实验组的细胞数显著多于对照组.与对照组相比,实验组的G1％(处于G1期的细胞百分率)降低,S％(处于S期的细胞百分率)和PrI值(增殖指数)升高.实验组MDSCs各亚周期(G1期、S期和G2M期)时间分别为21.5 h、4.4h和10.8 h,对照组MDSCs各亚周期时间分别为27.1h、9.6h和13.5 h.与对照组相比,实验组细胞G1期和S期明显缩短.ATP作用后,小鼠MDSCs中P2Y1受体表达显著升高,在3d时达到最高水平,之后开始下降,但仍然维持高水平.结论 ATP上调MSCs中P2Y1受体的表达,缩短G1期和S期,促使细胞从G1期进入S期,从而刺激MDSCs增殖.     

丹参抑制骨骼肌肌源性干细胞成纤维样分化作用的研究

目的 研究丹参对体外培养的骨骼肌肌源性干细胞(MDSCs)纤维样分化的作用.方法 采用差速贴壁法分离出大鼠MDSCs,利用TGF-β1诱导MDSC向成纤维样细胞分化,观察丹参对细胞成纤维样分化的影响.将体外培养的细胞分为3组:A组,对照组;B组,10 ng/ml TGF-β1组;C组,10 ng/ml TGF-β1+150 mg/L丹参组.在放大400倍相差显微镜下观察细胞生长情况,利用免疫细胞化学方法、RT-PCR、Western Blot检测MDSCs表达CTGF、COLⅠ、COL Ⅲ的情况.结果 在体外,TGF-β1能加强MDSCs表达结缔组织生长因子(CTGF)和胶原蛋白,丹参拮抗TGF-β1的作用,降低细胞CTGF、COLⅠ、COL Ⅲ mRNA的表达水平,降低CTGF、COLⅠ、COL Ⅲ蛋白的水平.结论 丹参可以抑制TGF-β1诱导MDSCs成纤维样分化作用.其机制可能是抑制CTGF表达,从而降低细胞合成和分泌胶原纤维,抑制细胞成纤维样分化.

Abstract：

Objective To investigate the effect of Salvia miltiorrhiza on fibroblastic differentiation of skeletal muscle-derived stem cells (MDSCs) in vitro. Methods MDSCs in rats were isolated by the method of adhering to culture plastic at different time points. Fibroblastic differentiation of MDSCs was induced by TGF-β1 administration and the effects of Salvia miltiorrhiza on fibroblastic differentiation were observed. The cells cultivated in vitro were divided into three groups according the supplement of TGF-β1 and Salvia miltiorrhiza in culture media: group A (control group) had no TGF-β1 and Salvia miltiorrhiza;group B had 10 ng/ml TGF-β1;group C had 10 ng/ml TGF-β1 and 150 mg/L Salvia miltiorrhiza. Cell growth was observed using phase contrast microscope. The expressions of CTGF,COLⅠand COL Ⅲ in each group were detected by immunocytochemistry,RT-PCR and Western Blot. Results In vitro,TGF-β1 promoted the expression of CTGF and collagens of MDSCs. However,the effect of TGF-β1 could be inhibited by Salvia miltiorrhiza,resulting in decreased mRNA and protein expression of CTGF,COLⅠand COL Ⅲ. Conclusion Salvia miltiorrhiza could inhibit fibroblastic differentiation of MDSCs induced by TGF-β1. The mechanism might be that inhibition of CTGF expression reduces the synthesis and secretion of collagen fibers and in turn inhibits the fibroblastic differentiation.     

健康老年人QT间期离散度的检测及其相关因素分析探讨

目的建立健康老年人12导联同步心电图QT间期离散度平均值及其相关因素分析。方法对1028例65～95(69.4±17.3)岁健康老年人,用SR-1000A心电综合自动分析仪采集12导联同步体表心电图数据,并录入软盘,专人在显示器上进行回放分析。增益放大1mV=20～40mm,纸速放大50～100mm/s。测量QTmax、QTmin,按Bazett公式校正QTcmax、QTcmin,计算QTd(QTmax-QTmin)及QTcd(QTcmax-QTcmin)。微机数理统计。结果QTmax、QTmin、QTcmax、QTcmin、QTd及QTcd平均值随增龄呈增大趋势,但在年龄组间及男女间差别无统计学意义。65～95岁QTmax平均值为399.39±32.93ms,QTmin为364.99±31.06ms,QTcmax为445.87±28.21ms,QTcmin为406.60±29.84ms,QTd为33.97±11.15ms,QTcd为38.14±12.84ms。结论健康老年人各年龄QTd为12～50ms,QTcd为13～60ms。QTd与心率、年龄及性别无明显相关性。