主动免疫疗法在原因不明习惯性流产患者中的应用

Objective To explore the relationship between the changes in cellular immunity and the causes of unexplained habitual abortion(UHA)as well as the mechanism of active immunotherapy on UHA patients.Methods T-lymphocyte and natural killer(NK)cell subsets(CD3+%,CD4+%,CD8+%,CD16+CD56+% and CD4+/CD8+ ratio)of peripheral blood were detected by applying flow cytometry and compared in 66 cases of UHA(UHA group,including 30 cases receiving active immunotherapy and 36 untreated UHA cases)and 30 cases of healthy fertile women(healthy control group).The re-pregnancy achievement ratio was compared between 30 treated UHA cases and 36 untreated UHA cases.Results The percentages of CD31+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly higher in UHA group than those of healthy control group(P＜0.050).As compared with those of untreated UHA subgroup,the percentages of CD3+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly lower(P＜0.050),while the re-pregnancy achievement ratio was higher(92.86% vs 05 29.03%,P＜0.001).Conclusion The changes in T-lymphocyte and NK cell subsets have something to do with occurrence of UHA.Active immunotherapy can regulate the cellular immune function and improve the re-pregnancy achievement ratio effectively.     

实时荧光探针定量PCR在小儿沙眼衣原体肺炎诊断中的临床应用研究

目的观察荧光定量PCR在小儿沙眼衣原体肺炎诊断中的应用价值及探讨不同年龄阶段小儿沙眼衣原体(CT)肺炎的临床特点。方法将我院2004年1~12月份收治60例小儿CT肺炎做研究对象,根据年龄分组,对各组患儿均通过咽拭子或吸痰法获取标本,进行CT—DNA定量分析;回顾性总结分析60例CT肺炎。结果60例标本中,106拷贝数5例,105拷贝数6例,104拷贝数10例,103拷贝数39例;小婴儿CT肺炎往往无发热性肺炎,易合并细菌感染;3岁以上儿童则易出现发热、肺部体征大多阴性,而X线多表现为节段性肺炎,其临床经过类似支原体肺炎。结论1.实时荧光定量PCR检测CT—DNA是诊断小儿沙眼衣原体肺炎最直接、最客观的指标。2.CT肺炎在不同年龄阶段的临床表现存在显著差异。     

层流手术中人员流动次数与空气含菌量的关系

目的了解并评价层流手术室中人员流动对空气含茵量的影响。方法采用平板暴露法对20例手术进行术中空气采样,采样时间从层流30min后手术患者入室前开始至手术结束,每隔30min采样计数空气细菌;同时记录各时间段内人员流动次数。结果层流手术室中各时间段平均人员流动次数及空气菌落数的相关系数为0．945,空气细菌含量与人员流动具有相关性,术中人员流动累计达60人次时空气微生物超过国家规定的普通手术室空气静态标准。结论术中人员流动的次数,直接影响层流手术室空气中的细菌含量,层流手术室术中必须严格限制入室人员。     

主动免疫疗法在原因不明习惯性流产患者中的应用

Objective To explore the relationship between the changes in cellular immunity and the causes of unexplained habitual abortion(UHA)as well as the mechanism of active immunotherapy on UHA patients.Methods T-lymphocyte and natural killer(NK)cell subsets(CD3+%,CD4+%,CD8+%,CD16+CD56+% and CD4+/CD8+ ratio)of peripheral blood were detected by applying flow cytometry and compared in 66 cases of UHA(UHA group,including 30 cases receiving active immunotherapy and 36 untreated UHA cases)and 30 cases of healthy fertile women(healthy control group).The re-pregnancy achievement ratio was compared between 30 treated UHA cases and 36 untreated UHA cases.Results The percentages of CD31+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly higher in UHA group than those of healthy control group(P＜0.050).As compared with those of untreated UHA subgroup,the percentages of CD3+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly lower(P＜0.050),while the re-pregnancy achievement ratio was higher(92.86% vs 05 29.03%,P＜0.001).Conclusion The changes in T-lymphocyte and NK cell subsets have something to do with occurrence of UHA.Active immunotherapy can regulate the cellular immune function and improve the re-pregnancy achievement ratio effectively.     

主动免疫疗法在原因不明习惯性流产患者中的应用

Objective To explore the relationship between the changes in cellular immunity and the causes of unexplained habitual abortion(UHA)as well as the mechanism of active immunotherapy on UHA patients.Methods T-lymphocyte and natural killer(NK)cell subsets(CD3+%,CD4+%,CD8+%,CD16+CD56+% and CD4+/CD8+ ratio)of peripheral blood were detected by applying flow cytometry and compared in 66 cases of UHA(UHA group,including 30 cases receiving active immunotherapy and 36 untreated UHA cases)and 30 cases of healthy fertile women(healthy control group).The re-pregnancy achievement ratio was compared between 30 treated UHA cases and 36 untreated UHA cases.Results The percentages of CD31+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly higher in UHA group than those of healthy control group(P＜0.050).As compared with those of untreated UHA subgroup,the percentages of CD3+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly lower(P＜0.050),while the re-pregnancy achievement ratio was higher(92.86% vs 05 29.03%,P＜0.001).Conclusion The changes in T-lymphocyte and NK cell subsets have something to do with occurrence of UHA.Active immunotherapy can regulate the cellular immune function and improve the re-pregnancy achievement ratio effectively.     

循环活化血小板的检测在脑梗死患者诊治中的临床应用

目的探讨脑梗死（c1）患者循环血小板的活化情况及其与脑梗死的关系。方法以纤维蛋白原受体（FIB-R）和P-选择素作为分子标志物，应用流式细胞术检测121例脑梗死患者急性发作期以及恢复期循环活化血小板膜表面FIB—R和P-选择素的改变，70名健康体检者作为对照。结果c1患者急性期较恢复期及对照组FBI—R表达有统计学意义（P〈0．01）。髓患者不同梗死面积各组织间表达差异均无统计学意义（P〉0．05）。结论循环活化血小板与CI的发生、发展密切相关。FBI—R可作为反映cI患者循环活化血小板活化状态的一个敏感指标。动态检测CI患者FBI—R，一方面有助于患者病情轻重程度的判定，另一方面可以了解患者体内血小板的活化情况，及时调节抗血小板治疗，以便改善脑梗死的预后。     

主动免疫疗法在原因不明习惯性流产患者中的应用

Objective To explore the relationship between the changes in cellular immunity and the causes of unexplained habitual abortion(UHA)as well as the mechanism of active immunotherapy on UHA patients.Methods T-lymphocyte and natural killer(NK)cell subsets(CD3+%,CD4+%,CD8+%,CD16+CD56+% and CD4+/CD8+ ratio)of peripheral blood were detected by applying flow cytometry and compared in 66 cases of UHA(UHA group,including 30 cases receiving active immunotherapy and 36 untreated UHA cases)and 30 cases of healthy fertile women(healthy control group).The re-pregnancy achievement ratio was compared between 30 treated UHA cases and 36 untreated UHA cases.Results The percentages of CD31+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly higher in UHA group than those of healthy control group(P＜0.050).As compared with those of untreated UHA subgroup,the percentages of CD3+ and CD16+ CD56+ cells as well as CD4+/CD8+ ratio were significantly lower(P＜0.050),while the re-pregnancy achievement ratio was higher(92.86% vs 05 29.03%,P＜0.001).Conclusion The changes in T-lymphocyte and NK cell subsets have something to do with occurrence of UHA.Active immunotherapy can regulate the cellular immune function and improve the re-pregnancy achievement ratio effectively.     

小儿沙眼衣原体肺炎60例临床分析

沙眼衣原体（CT）是一组专一细胞内寄生的原核型微生物。是小儿下呼吸道感染的病原体之一。现将我院2004年1—12月收治的60例小儿CT肺炎依据年龄进行分组，以探讨不同年龄阶段小儿CT肺炎的临床特点。     

中西医治疗慢性肾功能衰竭的临床观察与护理

慢性肾功能衰竭为慢性肾炎的晚期阶段。以肾功能持久性减退 ,代谢产物潴留 ,水、电解质酸碱平衡失调为主要临床表现。很多人需要坚持长期服药与透析。由于我院地处经济不发达地区 ,很多病人透析难以继续。近年来 ,我科采用中西医结合治疗 ,辨证施护 ,通过 2 8例临床观察 ,取得了满意疗效。现总结如下 :1　资料与方法1.1　一般资料采用随机分组将 5 6名住院患者分为单纯西药治疗组 (对照组 )和中西医结合组 (治疗组 )各 2 8例。对照组男 16例 ;女 12例 ;年龄 18～ 6 0岁。治疗组男18例 ,女 10例 ;年龄 16～ 80岁 ;血 BUN(15 .2～ 30 .1)mmo…