某农药厂废水排放沟底泥中有机污染物的气相色谱－质谱分析

某农药厂废水排放沟底泥中有机污染物的气相色谱－质谱分析山东省卫生防疫站（济南，２５００１４）曹艳平，卫煜英用气相色谱－质谱联用技术对某农药厂废水排放沟底泥中有机物进行了分析测定，该方法操作简便、步骤少、省时间，适于测定多种有机污染物。１方法１．１样品...     

不同形式大鼠肝脏标本在压痕松弛实验中的黏弹性参数的差异

目的通过压痕松弛实验,研究三种形式大鼠肝脏的黏弹性参数的差异,探索黏弹性参数的影响因素。方法雄性SD大鼠10只,体重(180±20) g,10%水合氯醛3 ml/kg腹腔注射麻醉后,每只大鼠的肝脏依次在三种检测条件(在体检测、离体检测、将肝脏切成小块后体外检测)下分别进行压痕松弛实验,进行肝脏黏弹性的力学检测。使用Prony模型分别获取3种检测条件下的黏弹性参数:τ_1、τ_2、g_1、g_2。结果在体肝脏的黏弹性参数g_1、g_2、τ_1、τ_2分别为0. 508±0. 071、0. 194±0. 055、0. 754±0. 218、23. 524±9. 233,离体肝脏的黏弹性参数g_1、g_2、τ_1、τ_2分别为0. 384±0. 072、0. 256±0. 041、1. 062±0. 250、29. 254±2. 852,肝脏组织块的黏弹性参数g_1、g_2、τ_1、τ_2分别为0. 359±0. 030、0. 246±0. 027、1. 070±0. 137、30. 204±3. 250。离体肝脏及肝脏组织块与在体肝脏的黏弹性参数差异具有统计学意义(P 0. 05)。结论离体肝脏和肝脏组织块的黏弹性与在体肝脏的黏弹性有着较大差异,对肝脏的黏弹性进行研究时,应注意不同检测状态对肝脏黏弹性的影响。     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

气相色谱—质谱联用法分析水中有机污染物

随着环境污染的日益加重，关于自来水水中有机污染物的分析研究国内外已有不少报道[1-3]。自来水水中有机污染物含量低，成分复杂，故水样中有机污染物的富集是关键问题，液相溶剂萃取法简便易行但不适合处理大量的水样，现在广泛采用大孔网状高分子树脂小球吸附富集，然后经溶剂洗脱，以GC／MS或GC分析。”进口XAD系列树脂对水样中有机物的吸附已得到广泛认可，但其价格昂贵，本文以廉价易得的国产GDX系列大孔网状树脂富集水样[4]，经与XAD—2树脂比较吸附效果无明显差异，以二氯甲烷为洗脱溶剂，洗脱液经浓缩后，进行GC／MS分析，…     

寿光市1起盐酸克伦特罗引起的食物中毒调查

[目的]调查1起猪肝引起盐酸克伦特罗中毒的情况。[方法]对猪肝中盐酸克伦特罗采用GB/T 5009.192-2003第一法《气相色谱-质谱内标法》进行分析测定。[结果]在猪肝中检出盐酸克伦特罗,含量为231μg/kg,中毒症状与食用猪肝量有关。[结论]这是1起由盐酸克伦特罗引起的中毒。     

乙型肝炎患者为什么必须检查HBV-DNA?

一直以来,无论是正常人群还是乙肝患者,“乙肝两对半”（HBsAg、HBsAb、HBeAg、HBeAb、HBcAb）项目检测已经被普遍接受。“乙肝两对半”是判断是否感染乙肝,区分乙肝“大三阳”和“小三阳”的重要指标,也是指导正常人群强化乙肝疫苗接种的指标。     

恩替卡韦单药和联合胸腺肽对代偿期乙肝肝硬化患者APRI和LSM值的影响

目的：通过比较恩替卡韦（entecavir,ETV）单药和联合胸腺肽（thymosin,Thyα-a）对慢性乙肝肝纤维化患者APRI和LSM值的疗效,优化抗乙肝病毒治疗方案。方法：代偿期乙肝肝硬化患者72例,随机分为ETV组34例,ETV+Thyα-a组38例。ETV组给予ETV分散片,0.5mg/次,1次/日,疗程78周;ETV+Thyα-a组在服用ETV分散片的基础上,于26周后联合Thyα-a,1.6mg/次,2次/周,皮下注射,治疗52周,疗程78周。检测治疗前及治疗后26、52、78周患者血小板计数和天冬氨酸氨基转移酶活性,采用FibroTouch超声诊断仪测定LSM值。结果：两组患者的APRI均呈总体下降趋势,其中以0～26周下降最为明显,26、52、78周的APRI均低于0周,差异均有统计学意义（P＜0.01）。两组APRI均呈总体下降趋势,均以0～26周下降最为明显;26、52、78周的APRI均低于0周,差异均有统计学意义（P＜0.01）;78周的LSM值低于26周,差异有统计学意义（P＜0.05）。ETV+Thyα-a组患者的病毒定量呈总体下降趋势,26、52、78周病毒定量均低于0周,差异均有统计学意义（P＜0.02）。而ETV组在0～26周下降明显,但在52周、78周逐渐升高。ETV组各访视点间的病毒定量差异均无统计学意义（P>0.05）。两组在同一访视点的病毒定量差异均无统计学意义（P>0.05）。结论：ETV单药和ETV+Thyα-a联合用药能在治疗26周内迅速降低代偿期乙肝肝硬化患者的APRI和LSM值,持续抗病毒治疗可将APRI、LSM值和病毒定量控制在较低水平,但是两种治疗方案间并无明显的疗效差异。     

工作场所空气中6种氨基甲酸酯的气相色谱测定法

目的建立工作场所空气中6种氨基甲酸酯的气相色谱测定法。方法以ZK-2空气采样器采集空气,以丙酮解吸,以HP-5毛细管色谱柱分离,流动相为氮气,流量为26 ml/min,氢气流量为30 ml/min,空气流量为60 ml/min,程序升温,进行气相色谱分析以检测工作场所空气中6种氨基甲酸酯浓度。结果该方法中6种氨基甲酸酯的线性范围为0.1～100.0μg/ml,相关系数为0.999 4～0.999 9,回收率为90%～99%,检出限为0.02～0.10μg/ml,精密度为1.4%～5.6%。结论该方法简便,快速,准确,适用于工作场所空气氨基甲酸酯的测定。     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.