160例非单纯性阑尾炎的诊治体会

目的 探讨化脓性、坏疽性及穿孔性阑尾炎、阑尾周围脓肿的诊断体会和手术治疗意义.方法 对笔者所在医院2003年6月至2007年12月间手术治疗非单纯性阑尾炎患者的临床资料进行回顾性分析.结果 160例手术患者中治愈159例.结论 手术治疗是治疗非单纯性阑尾炎的一种安全、有效的治疗手段.     

巢式PCR检测先天性心脏病心脏石蜡标本中B19病毒的感染

目的：探讨微小病毒Ｂ１９与先天性心脏病（ＣＨＤ）的相关性及可能致畸机理。方法：采用病例对照研究，病例组为２９例ＣＨＤ尸解心脏组织，对照组为３０例同期非先天性畸形尸解心脏组织，应用巢式ＰＣＲ扩增Ｂ１９－ＤＮＡ，单纯疱疹病毒（ＨＳＶ），兔弓形虫（ＴＯＸ），巨细胞病毒（ＣＭＶ），结果：２９例ＣＨＤＢ１９－ＤＮＡ５例阳性，全瓿对照组为均阴性（Ｐ＝０．０２３７），ＨＳＶ，ＴＯＸ两组中均阴性，ＣＭＶ在两组中均     

异体紫外线充氧血佐治小儿重症肺炎及其血氧饱和度,体液免疫？…

采用异体紫外线充氧血佐治小儿重庆肺炎７８例，用美国Ｎｅｌｌｃｏｒ ２５０Ｅ型无创血氧，心电及脉搏监护仪监测。对１６例做了车注前后体液免疫指标检测。结果显示：患儿除临床症状明显改善外，总治愈率达９２．３１％，明显优于对照组；治疗前后经皮无创血氧饱和度，ＩｇＧ均有明显上升；     

西安地区呼吸道感染患儿肺炎链球菌携带和耐药情况分析

目的分析陕西省西安地区急性呼吸道感染儿童鼻咽部肺炎链球菌 (SP)携带及耐药情况。方法采集2001年8～10月门诊就诊、年龄1个月～5岁、急性呼吸道感染、未使用或使用抗生素不超过3d共234例患儿的鼻咽部分泌物 ,分离培养SP ,并利用纸片扩散法进行抗生素敏感性检测。结果急性呼吸道感染患儿鼻咽部SP携带率为28.2 % ,年龄主要为1～4岁 ,家中居住、就诊前未使用抗生素的儿童携菌率较高。共分离出66株SP ,其中100 %对氨苄西林、阿莫西林/棒酸、头孢呋辛、头孢曲松、左氧氟沙星和万古霉素敏感 ,对阿奇霉素和克拉霉素耐药率>80% ,对复方新诺明的耐药率为54 %。结论西安地区SP在急性呼吸道感染儿童中的携带率近1/3 ,对多种抗生素有不同耐药性和敏感性 ,以对阿奇霉素、克拉霉素、复方新诺明等多重耐药为主要特点。     

皮肤病学与公共卫生

皮肤病学与公共卫生密切相关,本文拟通过阐述皮肤病学与公共卫生间的关系,强调皮肤科医师必须重视皮肤病的预防与控制,树立公共卫生观点,为全人类的皮肤保健作出贡献。WHO于1987年就提出了到2000年“人人享受卫生保健”的战略目标,但这个目标忽视了皮肤保健,随着世界经济的发展     

B19病毒对先天性心脏病先天感染的探证及电镜观察

目的 证实部分先天性心脏病（CHD）患者心肌组织中微小病毒B19系先天感染所致,并观察CHD心肌组织超微结构变化。方法 采用ELISA,PCR,原位杂交（ISH）及透射电镜等技术,对42例CHD患者及对照组22例风湿性心瓣膜病患者的外周血,活检心肌组织分别进行B19－VP2－Ig M,B19-DNA的检测,并用透射电镜对心肌细胞的超微结构进行观察。结果（1）CHD组中B19－VP2－IgM阳性3例（7％）,对照组中阳性1例（5％）,两组间无显著差异（P＞0．05）,（2）CHD组中B19－DNA阳性8例（19％）,与对照组均阴性相比有显著性差异（P＜0．01）,且与8例心肌B19－DNA阳性标本配对的血中B19－VP2－IgM的均阴性。（3）ISH显示B19DNA定位于心肌细胞核内,电镜超微结构观察细胞胞质,核内均未见23nm病毒样颗粒,也无细胞器的损害。结论 B19病毒对先天性心脏病的感染系先天性感染所致,病毒基因可能整合于宿主细胞核内。     

RELATIONSHIP BETWEEN HUMAN PARVOVIRUS B19 INFECTION AND APLASTIC ANEMIA

Objective. To explore the relationship between human parvovirus B19 (HPV B19) infection and aplastic anemia (AA) and to investigate the role of HPV B19 in the occurrence of AA. Methods. The presence of HPV B19 DNA was detected in the peripheral blood samples of 60 patients with AA (children 38 and adults 22) by nested polymerase chain reaction (PCR) assay, and 30 healthy persons were selected as control. Results. Sixteen (26. 7 % ) of 60 AA cases were HPV B19 DNA positive, while all the samples in the control group were negative for HPV B19 (P = 0. 000914). Among the case group, the positive rates of HPV B19 DNA were21.4% (6 /28), 30.0% (3 / 10), 20.0% (1 /5) and 35.3% (6 / 17) in children acute AA (AAA), children chronic AA (CAA), adults AAA and adults CAA patients respectively, which were significant-ly higher than that in the control group, Furthermore, there was no remarkable difference between children AA and adults AA in the 16 HPV B19 DNA positive patients; neither was there between AAA and CAA. Conclusions. HPV B19 infection is not only correlated with the occurrence of children AAA and CAA, but also with adults AAA and CAA, and might be an important viral cause for AA in humans.     

人细小病毒B_(19)相关神经系统疾病

<正> 人细小病毒B_(19)(Human parvovirus B_(19),HPVB_(19))系动物细小病毒属中唯一致人类疾病的一种小DNA病毒,可引起传染性红斑,一过性再障危象、血小板减少性紫癜、关节炎、胎儿水肿、死胎、流产等多种疾病。随着研究的深入,HPVB_(19)感染致人类疾病谱在不断扩大。HPVB_(19)相关神经系统疾病相对较为少见,主要包括中枢神经病变如脑炎、脑膜炎以及周围神经病变如神经炎、肌萎缩等。本文就HPVB_(19)的生物学特征,脑病患者HPVB_(19)基因组特点,HPVB_(19)相关神经系统疾病的发病机理、临床表现、诊断、防治等方面综述如下。     

FINAL REPORT ABOUT STAGE Ⅱ COLLABORATION PROJECT ON LEPROSY REHABILITATION

Objective. To undertake the pilot experiments of prevention of disability (POD) in 14 different geographical areas to serve as examples for future development of rehabilitation work in China and in Asia. Mahods. According to the principles mid national criterion, 27 000 people affected by leprosy were selected and assessed using disability record forms at beginning and followed up regularly for observing changes of different indica-tors. Results. A total of 197 neuritis cases were detected and treated with prednisolone out of 1 407 new or active cas-es. Serf-care training of eyes, hands and feet were conducted for 10 500 disabled people affected by leprosy. Compre-hensive therapy was given to 1 804 cases having comphcated ulcers of which 1 055 cases have got their ulcers healed. Out of 706 prostheses, 613 were given to patients with satisfactory results. Surgical treatment was given to 269 cases and 251 have shown good progress. Conclusion. Most of patients have got benefit from the project in function or appearance which is very helpful for their going back to the society and agreed by foreign experts during the final evaluation. The experiences from the pro-ject can be implemented in the whole country.     

过敏性紫癜患儿人细小病毒B19感染

目的　探讨过敏性紫癜 (HSP)与人细小病毒B19(B19)感染的关系及其临床特征。方法　采用巢式PCR法和特异性抗体ELISA法对 5 5例HSP患儿血清标本进行B19 DNA、IgM、IgG检测。 结果　 1.HSP 5 5例中B19 DNA、IgM、IgG阳性率分别为 18% (10 / 5 5 )、14 % (8/ 5 5 )、16 % (9/ 5 5 ) ,与对照组比较无显著差异 (P >0 .0 5 )。 2 .HSP 5 5例中 2 4例有腹症 ,其中 5例可能与B19感染有关 ;34例有关节表现 ,其中 8例可能与B19感染有关 ;30例有肾损 ,其中 5例可能与B19感染有关。 3.并腹症或关节症状B19感染率分别达 2 0 .8%和2 3.5 % ,同时并两个症状 14例中 4例 (2 8.6 % )与B19感染有关 ,皮肤紫癜 腹症 关节 肾损 6例中 2例(33.3% )与B19感染有关。结论　 1.B19不是HSP患儿的主要病因 ,但可能是重要的病因之一 ;2 .B19感染相关性HSP临床表现多样 ,症状重 ,关节症状和腹症更突出。