全文获取类型
收费全文 | 1237篇 |
免费 | 96篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 30篇 |
妇产科学 | 8篇 |
基础医学 | 143篇 |
口腔科学 | 25篇 |
临床医学 | 62篇 |
内科学 | 286篇 |
皮肤病学 | 24篇 |
神经病学 | 110篇 |
特种医学 | 27篇 |
外科学 | 244篇 |
综合类 | 5篇 |
预防医学 | 21篇 |
药学 | 141篇 |
肿瘤学 | 212篇 |
出版年
2023年 | 12篇 |
2022年 | 6篇 |
2021年 | 26篇 |
2020年 | 22篇 |
2019年 | 18篇 |
2018年 | 42篇 |
2017年 | 29篇 |
2016年 | 33篇 |
2015年 | 44篇 |
2014年 | 41篇 |
2013年 | 46篇 |
2012年 | 94篇 |
2011年 | 89篇 |
2010年 | 36篇 |
2009年 | 42篇 |
2008年 | 53篇 |
2007年 | 62篇 |
2006年 | 69篇 |
2005年 | 72篇 |
2004年 | 55篇 |
2003年 | 72篇 |
2002年 | 45篇 |
2001年 | 35篇 |
2000年 | 44篇 |
1999年 | 37篇 |
1998年 | 16篇 |
1997年 | 5篇 |
1996年 | 12篇 |
1995年 | 7篇 |
1994年 | 6篇 |
1993年 | 8篇 |
1992年 | 12篇 |
1991年 | 14篇 |
1990年 | 18篇 |
1989年 | 12篇 |
1988年 | 12篇 |
1987年 | 11篇 |
1986年 | 10篇 |
1985年 | 10篇 |
1984年 | 5篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1977年 | 8篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1971年 | 4篇 |
1967年 | 7篇 |
1966年 | 9篇 |
1963年 | 2篇 |
排序方式: 共有1343条查询结果,搜索用时 248 毫秒
1.
Soroku Doko M.D. F.I.C.A. Tatsuki Katsumura M.D. F.I.C.A. Takashi Fujiwara M.D. Hiroshi Inada M.D. Hisao Masaki M.D. 《The International journal of angiology》1995,4(1):55-60
One hundred and thirty-four patients with vasoocclusive diseases were retrospectively tested for three kinds of antiphospholipid antibody (aPL). The mean age at onset of the disease in 58 patients with aPL was 43 years old. Seventeen, 11, and 9 patients were positive for the aCL IgA, IgM, and IgG isotypes, respectively. The rates of anti-phospholipid syndrome (APS) in patients with arterial (n=94), venous (n= 31), or both arterial and venous (n=9) occlusion were 45%, 29%, and 78%, respectively.The rates of APS in patients with autoimmune disease (n=13), thromboangiitis obliterans (TAO) (n= 36), arteriosclerosis obliterans (ASO) with lower leg involvement (n=8) or aortic arch syndrome (n=5), Raynaud's syndrome (n=15), aortitis syndrome (n= 13), ischemic heart disease (IHD) with young onset (n =12), and bilateral leg deep venous thrombosis (DVT) (n=10) were 77%, 46%, 13%, 80%, 40%, 62%, 33%, and 70%, respectively. The cumulative patency rate for reconstructive surgery in patients (n=13) with aCL was found to be considerably lower than that in those without aCL (n=13). From these results it was concluded that IgA was the most valuable aCL isotype for the diagnosis of APS and that aPL should be examined in patients with double-vessel occlusion, autoimmune disease, bilateral leg DVT, aortic arch syndrome, TAO, Raynaud's syndrome, or IHD with young onset. Furthermore, prophylaxis for graft failure is more strongly recommended for patients with aCL than for those without it. 相似文献
2.
Y Mizutani T Hashimura T Kitayama T Toshimitsu M Nonomura 《Hinyokika kiyo. Acta urologica Japonica》1990,36(5):605-608
Primary bladder tumor is the most frequent malignant tumor in the field of urology, whereas the incidence of secondary bladder tumor from a distant organ is quite rare. We report here a 21-year-old female patient with metastatic bladder tumor from gastric cancer. She came to our hospital with a complaint of only bladder irritability. Cystoscopical and cytological examinations revealed rhabdomyosarcoma of the bladder. She did not respond to radiation therapy and combined chemotherapy, consisting of actinomycin D, vincristine and cyclophosphamide, and died 91 days after admission. Autopsy revealed a primary tumor of poorly differentiated scirrhus carcinoma of the stomach. Thus this was a quite rare case of metastatic bladder carcinoma characterized by bladder irritability without gastrointestinal symptoms. 相似文献
3.
4.
A Hiraoka J Ishikawa H Kitayama T Yamagami H Teshima H Nakamura H Shibata T Masaoka S Ishigami F Taguchi 《Bone marrow transplantation》1991,7(2):107-111
We used a thin-sectioning technique for the electron microscopic detection of viral particles within the cells of urinary sediments in three recipients who developed hemorrhagic cystitis after allogeneic bone marrow transplantation. Results of viral cultures of urine and electron microscopic (EM) observations on urinary sediments were consistent in only one recipient. In this recipient, EM observations revealed many viral particles within the cells of urinary sediments with diameter of about 80 nm corresponding to adenovirus, of which type 11 was produced in viral cultures. In one of the other two recipients many viral particles with a mean diameter of 41.6 nm corresponding to papovavirus were observed, but viral cultures using conventional cells were negative. Re-cultures using HEK cells produced polyomavirus BK. EM observation was a clue to the correct diagnosis. In the remaining recipient, no viral particles were observed within the cells of urinary sediments, suggesting the hemorrhagic cystitis to be of non-viral origin, despite a positive result of viral culture. These results suggest that a thin-sectioning technique on the cells of urinary sediments is important for the differential diagnosis between a viral-induced and non-viral hemorrhagic cystitis. 相似文献
5.
A case of severe right radicular ischias following embolization is reported. Gelfoam powder and pledgets were used for right internal iliac artery embolization in the case of intractable bladder cancer hemorrhage. It is supposed that previous radiotherapy and fine embolic material may be the contributing factors to this complication. 相似文献
6.
Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery. 总被引:3,自引:0,他引:3
J C Schaeffer C L Lin S Kitayama G R Uhl 《Brain research. Molecular brain research》1991,9(3):271-276
A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening is described. 相似文献
7.
8.
Amplification of FISH signals using intermittent microwave irradiation for analysis of chromosomal instability in gastric cancer. 总被引:4,自引:0,他引:4 下载免费PDF全文
Gastrointestinal tract tumours are notorious for their difficulty in relation to conventional cytogenetic analysis. In particular, necrosis, the presence of stromal inflammatory and other cells, and poor attachment of tumour cells have led to problems with the quality and reliability of cytogenetic preparations, even with the recently developed fluorescence in situ hybridisation (FISH) technique. Furthermore, background autofluorescence masks the weak hybridisation signals in the nuclei. To overcome this problem, brief microwave treatment was applied for the identification of centromeres by in situ hybridisation in gastric cancer cells. Using this technique, a panel of 17 centromeric specific alpha-satellite probes was used to detect chromosomal instability in these cells. Lymphocyte controls and cancer cells subjected to irradiation achieved the hybridisation threshold in 30 minutes, providing a significant difference when compared with the non-irradiated samples (mean (SD) frequency of diploid cells 97% (2.1%) v 76% (4.6%), respectively). Therefore, this protocol of intermittent microwave treatment is recommended as a simple, rapid, and highly reproducible technique for application to various types of probe. It also gives well defined hybridisation signals and reduces background "noise". 相似文献
9.
10.
Masamitsu Shirai Tatsuki Nagatsuka Makoto Tanaka 《Macromolecular chemistry and physics.》1978,179(1):173-179
The effect of polyanions on the formation of mixed dimers of methylene blue ( 1 ) and trypaflavine ( 2 ), methylene blue ( 1 ) and phenosafranine ( 3 ), and methylene blue ( 1 ) and pyronine G ( 4 ) was investigated spectrophotometrically. The following polyanions were used: poly(potassium styrenesulfonate) (PSS), poly(potassium vinyl sulfate) (PVS), and poly(sodium acrylate) (PAA). On addition of polyanions, the formation of mixed dimers was enhanced largely. Thermodynamic parameters inferred that the enhancement of the formation of mixed dimers in the presence of polyanions resulted from an entropic factor. 相似文献