全文获取类型
收费全文 | 1303篇 |
免费 | 94篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 53篇 |
妇产科学 | 19篇 |
基础医学 | 182篇 |
口腔科学 | 19篇 |
临床医学 | 208篇 |
内科学 | 220篇 |
皮肤病学 | 13篇 |
神经病学 | 64篇 |
特种医学 | 126篇 |
外科学 | 204篇 |
综合类 | 75篇 |
一般理论 | 1篇 |
预防医学 | 83篇 |
眼科学 | 10篇 |
药学 | 61篇 |
1篇 | |
中国医学 | 2篇 |
肿瘤学 | 60篇 |
出版年
2019年 | 10篇 |
2017年 | 13篇 |
2015年 | 39篇 |
2014年 | 51篇 |
2013年 | 40篇 |
2012年 | 31篇 |
2011年 | 43篇 |
2010年 | 33篇 |
2009年 | 28篇 |
2008年 | 44篇 |
2007年 | 44篇 |
2006年 | 58篇 |
2005年 | 39篇 |
2004年 | 33篇 |
2003年 | 37篇 |
2002年 | 27篇 |
2001年 | 26篇 |
2000年 | 25篇 |
1999年 | 30篇 |
1998年 | 19篇 |
1997年 | 18篇 |
1996年 | 24篇 |
1995年 | 24篇 |
1994年 | 32篇 |
1993年 | 20篇 |
1992年 | 22篇 |
1991年 | 27篇 |
1990年 | 23篇 |
1989年 | 27篇 |
1988年 | 26篇 |
1987年 | 29篇 |
1986年 | 24篇 |
1985年 | 41篇 |
1984年 | 18篇 |
1983年 | 20篇 |
1982年 | 13篇 |
1981年 | 28篇 |
1980年 | 25篇 |
1979年 | 43篇 |
1978年 | 24篇 |
1977年 | 30篇 |
1976年 | 14篇 |
1975年 | 15篇 |
1974年 | 26篇 |
1973年 | 21篇 |
1972年 | 25篇 |
1971年 | 14篇 |
1970年 | 13篇 |
1969年 | 15篇 |
1968年 | 9篇 |
排序方式: 共有1403条查询结果,搜索用时 31 毫秒
1.
2.
Ulrich Weber Christian WA Pfirrmann Rudolf O Kissling Juerg Hodler Marco Zanetti 《BMC musculoskeletal disorders》2007,8(1):20
Background
Ankylosing spondylitis is a chronic inflammatory rheumatic disorder which usually begins in early adulthood. The diagnosis is often delayed by many years. MR imaging has become the preferred imaging method for detection of early inflammation of the axial skeleton in ankylosing spondylitis. 相似文献3.
4.
5.
6.
A L Harjula J C Baldwin N E Silverman N Blank P E Oyer E B Stinson N E Shumway 《The Journal of cardiovascular surgery》1990,31(1):1-6
Implantation response has been a critical problem following heart-lung and lung transplantation. While the precise etiology of this problem remains unclear, improvements in organ preservation would be expected to have a beneficial effect on implantation response. The time-related profile of the implantation response was studied in 20 patients who underwent heart-lung transplantation between March 1984-March 1987. In 10 operations the donors had intravenous prostaglandin E-1 pretreatment while 10 had no vasodilatation before explantation of the organs. Otherwise lung preservation and early (2 weeks) immunotherapy were similar in both groups. The implantation response was evaluated by chest films and postoperative lung functions and mechanics. Roentgenographic implantation response was evident from the first postoperative day, was less evident at the seventh postoperative day and then gradually increased during the second postoperative week. There was a tendency towards less implantation response in the PGE-1 group than in the control group, but no statistical difference was observed. Patients with severe operative bleeding problems were excluded from the study. Only peak inspiratory pressures were significantly higher in the control group than in the PGE-1 group (p less than 0.01). Other lung function studies (alveolar-capillary pO2 difference, extubation time) were not different in the groups. This study supported the hypothesis that prostaglandin E-1 may have salutary effects on graft preservation and implantation response in heart-lung transplantation. Since 1986, we have performed 16 heart-lung transplantations using graft preservation with PGE-1 and flush perfusion. Thirty-day mortality is 0% and 13 of 16 patients are surviving. 相似文献
7.
8.
目的:研究低氧时小鼠肺组织中低氧诱导因子-1α(HIF-k)表达的变化。方法:实验用雄性小鼠,低氧仓浓度分别为10%、7%、5%。用免疫荧光组织化学技术及共聚焦显微术,检测小鼠在低氧条件下肺组织中HIF-1α表达的变化。结果:正常组小鼠肺组织HIF-1α无表达,低氧组HIF-1α表达增加,且随低氧时间的延长及低氧强度的增加而增强。结论:低氧可诱导小鼠肺组织中HIF-1α的表达增强,(HIF-k)可能参与肺组织细胞凋亡的发生。 相似文献
9.
Generation of female genital tract antibody responses by local or central (common) mucosal immunization 总被引:6,自引:0,他引:6
Genital antibody responses were compared in female mice immunized intravaginally (i.vag.) or intranasally (i.n.) with a bacterial protein antigen (AgI/II of Streptococcus mutans) coupled to the B subunit of cholera toxin. Serum and salivary antibodies were also evaluated as measures of disseminated mucosal and systemic responses. Although i.vag. immunization induced local vaginal immunoglobulin A (IgA) and IgG antibody responses, these were not disseminated to a remote secretion, the saliva, and only modest levels of serum antibodies were generated. In contrast, i.n. immunization was substantially more effective at inducing IgA and IgG antibody responses in the genital tract and in the circulation, as well as at inducing IgA antibodies in the saliva. Moreover, mucosal and systemic antibodies induced by i.n. immunization persisted for at least 12 months. Analysis of the molecular form of genital IgA indicated that the majority of both total IgA and specific IgA antibody was polymeric, and likely derived from the common mucosal immune system. 相似文献
10.
Colonization of the cardiovascular endothelium by viridans group streptococci can result in infective endocarditis and possibly atherosclerosis; however, the mechanisms of pathogenesis are poorly understood. We investigated the ability of selected oral streptococci to infect monolayers of human umbilical vein endothelial cells (HUVEC) in 50% human plasma and to produce cytotoxicity. Planktonic Streptococcus gordonii CH1 killed HUVEC over a 5-h period by peroxidogenesis (alpha-hemolysin) and by acidogenesis but not by production of protein exotoxins. HUVEC were protected fully by addition of supplemental buffers and bovine liver catalase to the culture medium. Streptococci were also found to invade HUVEC by an endocytic mechanism that was dependent on polymerization of actin microfilaments and on a functional cytoskeleton, as indicated by inhibition with cytochalasin D and nocodazole. Electron microscopy revealed streptococci attached to HUVEC surfaces via numerous fibrillar structures and bacteria in membrane-encased cytoplasmic vacuoles. Following invasion by S. gordonii CH1, HUVEC monolayers showed 63% cell lysis over 4 h, releasing 64% of the total intracellular bacteria into the culture medium; however, the bacteria did not multiply during this time. The ability to invade HUVEC was exhibited by selected strains of S. gordonii, S. sanguis, S. mutans, S. mitis, and S. oralis but only weakly by S. salivarius. Comparison of isogenic pairs of S. gordonii revealed a requirement for several surface proteins for maximum host cell invasion: glucosyltransferase, the sialic acid-binding protein Hsa, and the hydrophobicity/coaggregation proteins CshA and CshB. Deletion of genes for the antigen I/II adhesins, SspA and SspB, did not affect invasion. We hypothesize that peroxidogenesis and invasion of the cardiovascular endothelium by viridans group streptococci are integral events in the pathogenesis of infective endocarditis and atherosclerosis. 相似文献