星宝贝，大制作

女明星集体“制造”星宝贝，让大家充满了欢喜与期待。[编者按]     

硬膜外阻滞麻醉病人术前访视调查

随着医学模式和护理观念的转变,术前访视是使手术得以顺利进行的必要步骤.它能为病人提供信息,缓解其焦虑心理[1],使病人了解手术部位及麻醉方式、术中体位,从而更好地配合手术麻醉及术后注意事项.     

生命的价值

有关死亡的哀痛。初次为我所体认是在遥远的1985年。关于如何得知不幸的讯息、如何辗转于冰凉秋夜的拥挤公车之种种情景，早溶干混沌过往而模糊不堪，只记得父母在路途中异乎寻常的沉默焦灼。于是便识趣地紧拽家长衣角，跟牢脚步，同样不发一语。     

乳腺癌患者血清肌酸激酶BB的放射免疫测定可作为一种肿瘤标记

作者用放免法在113例乳腺癌和354例女性供血者中检测血清脑型肌酸激酶(CPK-BB)含量.在放免检测时,与酶的MB型(不是MM型)有部份交义反应,但MB型有与稀释度不相平行的特点,据此可与酶的BB型区别.检测中批内变异为4.6%,而批间变异为10.3%.标本在采集后12小时内分离,此后或立即检测或贮存于-70℃达一周以上.对高含量患者的血清作一系列稀释后制成曲线,并与已检测的标准曲线比较.     

严重喉气管外伤的急救与护理

严重喉气管外伤是一种比较复杂的急症，急救与护理是否妥当可直接关系到病人的安危和预后。我科于2002～2003年共收治4例严重喉气管外伤急诊病例，现将护理体会报道如下。     

脂多糖刺激RAW264.7和Ana-1形态改变及细胞因子表达的差异

Objective To compare the morphologic change and cytokines expression in RAW264.7 and Ana-1 stimulated by lipopolysaccharide(LPS). Methods RAW264.7 and Ana-1 were cultivated with various concentrations of LPS(0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L). MTT was performed to evaluate the proliferation ability of cells. Two kinds of cells were cultivated with 1 mg/L. Then,the concentrations of TNF-α,IL-1β,IL-6,IL-10 in culture medium were detected by ELISA in different times(0 h,4 h,8 h,12 h,24 h). Results The survival rates of RAW264.7 and Ana-1 (162.05±28.14)% and (159.92±20.43)% were significantly higher in 1 mg/L group than those of other groups. When stimulated with 1 mg/L LPS, both RAW264.7 and Ana-1 expressed cytokines in time-dependent manner (increased first and decreased finally). The concentrations of TNF-α and IL-10 in RAW264.7 group were higher than those Ana-1 group in 4 h only. IL-1β and IL-6,however,were in higher concentrations in RAW264.7 group than Ana-1 group all the time. Conclusions When stimulated with 1 mg/L LPS, both RAW264.7 and Ana-1 have higher survival rate. RAW264.7 and Ana-1 have different ability in expressing cytokines after being stimulated by LPS. Therefore,RAW264.7 and Ana-1 have variant response to LPS slightly.