TCT和HPV-DNA配合阴道镜在宫颈病变中的临床意义

目的 探讨液基薄层细胞学检查技术(TCT)结合乳头瘤病毒定性检测(HPV-DNA)23型对宫颈癌前病变的诊断价值.方法 收集本院2006年8月至2007年8月妇科门诊TCT检查结合HPVt-DNA检测1128例,对TCT阳性、HPV阳性病例及临床高度怀疑者377例行阴道镜下活检.结果 随着细胞学诊断级别的升高,HPV感染率上升.随着病理诊断级别的升高,高危型HPV感染率上升,低危型HPV感染多见于CIN I及其以下病变.结论 采用TCT结合HPV-DNA分型初筛,异常者行阴道镜下活检,能提高病变检出率.对于HPV高危型感染者需密切随访.     

早孕合并黄体囊肿破裂27例临床分析

早孕合并黄体囊肿破裂在临床上表现为停经、腹痛、尿 /血ＨＣＧ检查阳性 ,常有不同程度的腹内出血 ,与宫外孕的症状十分相似 ,如果不仔细检查易发生误诊 ,影响治疗。本文在于探讨两者的鉴别、治疗及其相关问题。临　床　资　料1　病例来源　选自 1 985年 1月至 2 0 0 1年 3月在我院住院治疗的早孕合并黄体囊肿破裂的病例 2 7例。 1 1例手术治疗中术前有 7例误诊 ,1 6例保守治疗中有 4例误诊 ,共误诊 1 1例 ,误诊率为 40 .7%。2　临床情况　平均年龄 2 8岁 ( 2 1岁～ 3 5岁 ) ,未产妇 1 6例 ,经产妇 1 2例。全部病例都有停经史 ( 3 2天～ 58…     

温岭市中学女生继发性闭经病因及治疗评价

目的:该文以温岭市为例,首次报道中国中学女生继发性闭经的发病原因及治疗效果.方法:以2006-2010年在浙江省温岭市第一医院就诊妇科疾病的12至19岁女中学生为研究对象,通过详细询问病史、雌激素水平、孕激素水平、垂体促性腺激素水平、影像学检查及腔镜检查,确认患者发病原因,统计继发性患者人数及不同类型病因患者人数,对患者治疗9个月后观察治疗效果.结果:通过诊断确诊继发性闭经女生131人,占统计期间内就诊女生人数的8.22％.其中下丘脑性闭经占93.1％(122/131),卵巢性闭经占1.5％,药物性闭经占3.8％,子宫性闭经和其他原因引起的闭经各占0.8％.下丘脑性闭经治疗有效率为97.5％,其余各组闭经经治疗后均治愈.结论:下丘脑功能异常导致的继发性闭经是温岭市中学女生继发性闭经最主要的类型,绝大多数女中学生经治疗后可以恢复正常月经.     

剖宫产术中不同取头方法的应用分析

剖宫产术是产科领域重要的手术,现已成为解决难产和某些产科合并症、挽救产妇和围产儿生命的有效手段.     

冻干健康人血浆澄清度检查的吸收度控制法

<正> 冻干健康人血浆成品(以下简称血浆)用0.1％枸橼酸液溶解后呈黄色微混浊液体,由于产品质量和生产工艺的差异,形成的混悬度不尽相同,中国生物制品规程对此未作具体规定。国内普遍采用目视比浊法进行质量控制,这种方法误差较大,判别时的光线、背景的色深、操作者的辨浊视力等均带来直接影响;为此,我们根据混浊液在一定波长范围其浊度与吸收度呈线性关系,试用可见分光光度计     

高危妊娠患者剖宫产术后并发低氧血症的临床研究

目的 探讨高危妊娠患者剖宫产术后并发低氧血症的临床特征.方法 64例行剖腹产术的高危妊娠患者,其中28例并发低氧血症患者设为观察组,其余患者设为对照组（36例）,比较分析两组临床特征.结果 观察组肺血管阻力增加、呼吸频率增快、心率增快及意识消失等发生率均显著高于对照组（Х^2=7.142、9.346、7.003、4.898,均P＜0.05）,观察组切口愈合时间显著长于对照组（t=2.165,P＜O.05）.结论 高危妊娠患者剖宫产术后并发低氧血症的临床特征包括肺血管阻力增加、呼吸频率增快、心率增快、切口愈合时间延长及意识消失等多方面内容,临床工作中要提高警惕采取合适的防治措施,保障母婴安全.     

人乳头状瘤病毒与妇科其他常见病原微生物感染关系的调查

Objective To Investigate correlation between screening assay of human papillomavirus (HPV) and microbial pathogens in gynecology. Methods Cervical samples were collected to search for HPV, bacteria and yeast infections in gynecologic outpatients. HPV typing was carried out by PCR and sequencing on cervical brush specimens. Chlamydia trachomatis was identified by strand displacement amplification (SDA) and the other microorganisms were detected by conventional methods. All data were analyzed to investigate the correlation among them. Results In this cross-sectional study, among 857 enrolled outpatients, there were 266 cases with positive HPV DNA, and the rate of infection was 31.0%(266/857). HPV genotype showed that thirty-five different HPV types were identified, of which HPV16 was the most prevalent ( 14.5%, 38/262), followed by HPV58 (9.2%, 24/262), HPV53 (8.0%, 21/262)and HPV42 (6.1%, 16/262); while other genotypes were present in less than 5% of HPV positive women.According to the reclassification, the aggregated percentage (high-risk and probably high-risk) of detected HPV was 58.8% ( 154/262), 27.9% (73/262) for low-risk and 13.4% (35/262) for unknown-risk HPV types. Among HPV positive women, cervical brush specimens results showed that more than 60% cases with normal cytology, 3.8% (10/266) with high-grade squamous intraepithelial lesions (HSIL), 29. 7% (79/266) with low-grade squamous intraepithelial lesions (LSIL) and 3.0% (8/266) with atypical squamous cells of undetermined significance (ASCUS), respectively. Statistical analyses revealed there was a significant association between the infected HPV and Chlamydia trachomatis or Ureaplasma urealyticum ( >10000 CCU/ml; all P <0.01), while no correlation was found between HPV infection and bacterial vaginosis, streptococcus agalactiae, candida, Trichomonas vaginalis or Ureaplasma urealyticum ( ≤ 10000CCU/ml; all P > 0.05 ). Among the cases with bacterial vaginosis, the positive rate of HPV infected was42.6%. Chlamydia trachomatis was one of the high-risk factors for the infection of HPV ( OR = 2.82, 95%CI: 1.74 -4.57). Mycoplasma hominis was isolated only in 2 cases, no patient was infected with Neisseria gonorrhocae. Conclusions Although bacterial vaginosis was not significantly associated with HPV, it was more common among the HPV positive women. There is the significant association between HPV and Chlamydia trachomatis or Ureaplasma urealyticum which may be increase the infection of HPV. These data suggest that it may be important to screen for the simultaneous presence of different microorganisms which may have synergistic pathological effects.     

浅析GMP软件系统中标准操作规程(SOP)的制定方法

GMP软件系统主要包括生产管理、质量管理、技术管理、厂房设施和设备管理以及物料管理等五大系统。GMP软件系统构成按其性质可分为标准和记录两大部分,其中标准分技术标准、管理标准和工作标准,而标准操作规程(SOP)在软件系统中属于工作标准中的一类。因各国的GMP虽基本内容相似,但GMP并没有具体到每个企业应当如何做的地步,这就要求每个企业必需制定出各自实施规范的具体规定和要求,这些通常包括在标准操作规程内。因此,SOP是GMP规范中有关内容在某一特定企业的具体规定。     

不同方法终止中期妊娠的临床比较

目前应用于临床中孕引产的方法有多种,但各有其优缺点.本院自1999年1月～2004年12月曾采用3种不同的方法进行中孕引产,共165例引产病例.回顾性比较其各自的优缺点.