TCT、宫颈刮片及HPV-DNA检查在提示宫颈感染中的作用

液基薄层细胞学检查（ThinPrep Cytology Test，TCT）在检测宫颈上皮病变中的作用多有研究，但其在检查宫颈病原微生物中的作用少有报道。本文对LBTCT与常规宫颈刮片，HPV-DNA检查在提示宫颈感染病原微生物中的作用，结果如下。     

X-盒结合蛋白1在食管癌癌组织中的表达及对食管癌生物学行为的影响

【摘要】 目的 观察X-盒结合蛋匂1在食管癌组织中的表达及对食管癌细胞增殖、迁移、侵袭能力的影响。方法 60例食管癌与癌 旁组织,分别以Real-time PCR、western-blot、免疫组化检测XBP-1表达情况。在TE-1细胞系中,转染 pcDNA3. 1-XBP-1,检测TE-1细胞增殖、迁移、侵袭能力变化。结果 Real-time PCR检测结果显示,食管癌组织中 XBP-1 mRNA水平显著高于癌旁组织,差异有统计学意义(P<0. 05)。Western-blot检测结果显示,食管癌组织中XBP- 1蛋匂表达水平显著高于癌旁组织,差异有统计学意义(P<0.05) o 60例食管癌组织中,48例呈阳性表达,12例呈阴性 表达。60例癌旁组织中,15例呈阳性表达,45例呈阴性表达。食管癌组织中XBP-1阳性表达率显著高于癌旁组织 (P<0.05)。在TE-1细胞中,转染pcDNA3. 1-XBP-1组细胞增殖率显著高于空匂对照组及空载对照组(P<0. 05)o TE-1细胞中,转染pcDNA3. 1-XBP-1后迁移细胞数、侵袭细胞数均显著多于转染pcDNA3. 1及空匂对照组(P<0.05)。结论 XBP-1在食管癌中表达量显著高于癌旁组织,转染XBP-1基因可促进食管癌细胞TE-1增殖、迁移、侵袭。     

Effects of Aconitine on [Ca2＋] Oscillation in Cultured Myocytes of Neonatal Rats

In order to investigate the effects of aconitine on [Ca2 ] oscillation patterns in cultured myocytes of neonatal rats, fluorescent Ca2 indicator Fluo-4 NW and laser scanning confocal micro- scope (LSCM) were used to detect the real-time changes of [Ca2 ] oscillation patterns in the cultured myocytes before and after aconitine (1.0 μmol/L) incubation or antiarrhythmic peptide (AAP) and aconitine co-incubation. The results showed under control conditions, [Ca2 ] oscillations were irregu- lar but relatively stable, occasionally accompanied by small calcium sparks. After incubation of the cultures with aconitine, high frequency [Ca2 ] oscillations emerged in both nuclear and cytoplasmic regions, whereas typical calcium sparks disappeared and the average [Ca2 ] in the cytoplasm of the cardiomyocyte did not change significantly. In AAP-treated cultures, intracellular [Ca2 ] oscillation also changed, with periodic frequency, increased amplitudes and prolonged duration of calcium sparks. These patterns were not altered significantly by subsequent aconitine incubation. The basal value of [Ca2 ] in nuclear region was higher than that in the cytoplasmic region. In the presence or absence of drugs, the [Ca2 ] oscillated synchronously in both the nuclear and cytoplasmic regions of the same cardiomyocyte. It was concluded that although oscillating strenuously at high frequency, the average [Ca2 ] in the cytoplasm of cardiomyocyte did not change significantly after aconitine incuba- tion, compared to the controls. The observations indicate that aconitine induces the changes in [Ca2 ] oscillation frequency other than the Ca2 overload.     

N-myc下游调控基因2在消化道恶性肿瘤中的作用机制

N-myc下游调控基因2 (N-myc downstream regulated gene 2,NDRG2)是NDRG家族的一员,作为一种研究比较热门的抑癌候选基因,与细胞增殖、分化、凋亡和缺氧、脂肪毒性等应激反应及细胞迁移、侵袭等密切相关。全文阐述了NDRG2的分子特征及基因启动子甲基化、蛋白质磷酸化修饰等表观遗传作用机制,总结了NDRG2与长链非编码RNA(long noncoding RNA, LncRNA)、非编码单链RNA分子微小核糖核酸（MicroRNA,miRNA）、B淋巴细胞瘤-2(B-cell lymphoma-2, Bcl-2)及细胞连接相关基因E钙黏着蛋白/上皮性钙黏附蛋白（E-cadherin, E-Cad）等相互调控关系,系统地阐述了NDRG2在消化道恶性肿瘤：食管癌、胃癌及结直肠癌的发生发展中的作用机制。本文有助于进一步探索消化道恶性肿瘤的特异性诊断标记物和靶向药物的分子靶点,对消化道肿瘤的防治具有重要意义。     

不同年龄段已婚妇女甲襞微循环及宫颈病变结果分析

目的:观察不同年龄段已婚妇女甲襞微循环状况,并结合宫颈刮片和阴道镜检查结果,综合分析甲襞微循环在宫颈病变定性评估中的意义。方法:348例已婚妇女按年龄分为25～35岁,36～45岁和46～55岁三组,依次进行甲襞微循环形态、流态和袢周状态的检测积分,同时进行阴道镜检查和宫颈刮片检查。结果:甲襞微循环异常者主要集中在36～45岁年龄组,其积分值明显高于其它两个年龄组(P<0.05或P<0.01)。宫颈刮片细胞分类>Ⅱ级以及阴道镜检查结果异常者,也多出现于36～45岁年龄组,其异常率与其它两年龄组比较显著升高(P<0.01)。结论:存在明显宫颈病变妇女可发生甲襞微循环异常,在对宫颈病变定性评估中,甲襞微循环检测结果可以作为参考依据。     

脑胶质瘤患者预后影响因素分析

[目的]探讨脑胶质瘤患者预后影响因素,以提高胶质瘤患者术后生存率。[方法]外科手术后经病理确诊为脑胶质瘤的患者158例,查找病历和电话随访收集其资料,生存分析单因素分析使用Kaplan-Meier法计算生存率,并采用Log-rank检验;多因素分析采用Cox分析。[结果]单因素分析表明影响预后的因素有：年龄、肿瘤级别、KPS评分、术后放疗和化疗。多因素分析显示年龄、肿瘤级别、KPS评分、术后放疗和化疗是影响预后的独立因素。[结论]年龄小、级别低、KPS评分高的患者术后预后较好,术后辅助放疗或者化疗有助于改善预后。     

DC-CIK细胞免疫治疗的生物学基础及临床应用

细胞因子诱导的杀伤细胞(cytokine-induced killer cells,CIK)是一非主要组织相容性复合体(MHC)限制性的细胞毒性T淋巴细胞,被认为是肿瘤过继免疫治疗的新希望。而树突状细胞(dendritic cells,DC)是目前所知的人体内功能最强的抗原递呈细(antigen p resenting cell,APC),两者共培养后,显示出强大的协同抗肿瘤作用,已经广泛应用于部分肿瘤中,并取得了一定的临床疗效。