Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

35例甲肝患儿T淋巴细胞亚群检测——酶法测定

本文报道用免疫酶法对小儿甲肝35例血T淋巴细胞亚群进行了检测,以探讨甲肝病人细胞免疫功能的变化。1 材料和方法1.1 研究对象 为1989年12月～1990年3月在我院传染科住院的患儿,共35例,其中男22例,女13例,年龄2月～13岁,平均6.4岁,病人分别于急性期(症状明显、SGPT明显升高,浊度试验明显异常)及恢复期(症状消失、SGDT接近正常、浊度试验接近正常)采血测定T淋巴细胞亚群。所有研究对象均有肝功能异常,抗HAV-IgM阳性,乙肝全套指标及抗EBV-IgM,抗CMV-IgM均为阴性。以25例肝功能正常。HBsAg为阴性的正常儿童作为对照。     

缺氧诱导因子-1α和内皮素-1在胃肠道间质瘤中的表达及意义

目的 探讨缺氧诱导因子(HIF)-1α,血管内皮素(ET)-1在胃肠道间质瘤(GISTs)中的表达及其在预后判断中的价值.方法 应用免疫组织化学SP法对76例GISTs检测,观察HIF-1α、ET-1在GISTs中的表达情况.结果 HIF-1α、ET-1在GISTs中的阳性表达率分别为73.68%(56/76)、65.79(50/76),两者的表达呈正相关(P<0.05),其与胃肠道间质瘤的组织学分级、肿瘤直径、浸润转移和核分裂数有关(P<0.05),而与患者的年龄、性别、肿瘤原发部位无关(P>0.05);HIF-1α、ET-1在极低和低危险,中度危险和高危险及直径<2 cm和直径>5 cm组间差异有统计学意义(P<0.05),阳性表达率随恶性程度的增加和肿瘤直径的增大而增加(P<0.05),在有浸润转移和核分裂数≥5/50HP组中的表达明显高于无浸润转移和核分裂数<5/50HP组中的表达(P<0.05).结论 HIF-1α、ET-1在GISTs中的表达有联合倾向,可能提示肿瘤的恶性程度及其预后,并可作为判断预后的有用标志物.     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

腮腺炎并发脑膜脑炎的早期诊断

目的探讨腮腺炎并发脑膜脑炎的早期诊断依据.方法分析68 例脑膜脑炎的临床特点及脑脊液、脑电图改变并随访.结果 23.53%病人脑电图改变先于临床症状出现,表现为以弥漫性中高幅慢波为主,枕部及额部高幅θ活动,64.7%病人脑脊液有改变.结论脑脊液及脑电图有助于腮腺炎并发脑膜脑炎的早期诊断.     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.     

胃肠道间质瘤中E-cadherin和Ki-67的表达及临床意义

目的:探讨胃肠道间质瘤(GIST)中E-cadherin和Ki-67在GIST中的表达和意义,以及对GIST预后的影响.方法:回顾性分析2002年6月～2008年6月,我院78例GIST手术患者的临床和病理资料,分析其临床特征、病理分级以及免疫表达对GIST术后2年复发转移率和5年生存率的影响.结果:全组患者E-cadherin的阳性表达率为65.38%(51/78),Ki-67的阳性表达率为55.13%(43/78).E-cadherin和Ki-67的表达在不同性别和年龄组中无显著性差异(P>0.05),高危组与其他组比较,有显著性差异(P<0.05).肿瘤的原发部位、直径大小、核分裂象、E-cadherin和Ki-67的表达对肿瘤的预后影响显著(P<0.05).结论:E-cadherin及Ki-67在GIST中的表达与肿瘤的发展及预后密切相关,E-cadherin表达情况是独立预后因素,E-cadherin可作为预测术后转移及复发的可靠指标.     

大肠癌患者外周血淋巴细胞粘着斑激酶表达的临床意义

目的：探讨大肠癌患者外周血淋巴细胞粘着斑激酶(FAK)的表达及其临床意义。方法 结果 结论 方法：应用流式细胞仪(flow cytometry, FCM)检测40例大肠癌患者及20例正常人外周血淋巴细胞中FAK的表达。结果：大肠癌患者外周血淋巴细胞FAK阳性表达率为60.0%，与正常人(25.0%)比较，差异有显著性意义(P＜0.05)。并且外周血淋巴细胞FAK表达与大肠癌临床分期、组织学分化程度、淋巴转移有关。结论：大肠癌患者外周血淋巴细胞FAK异常表达与荷瘤状况有关，对大肠癌的早期诊断、临床分期、组织学分型、淋巴转移的预测等可能有一定帮助。     

2009—2015年湖南省肿瘤登记地区恶性淋巴瘤发病与死亡分析

摘 要：［目的］ 分析2009—2015年湖南省肿瘤登记地区恶性淋巴瘤发病、死亡情况。［方法］计算2009—2015年湖南省肿瘤登记地区恶性淋巴瘤的发病率、死亡率、0～74岁累积率、35～64岁截缩率等指标，标化率采用2000年全国人口普查标准人口和Segi’s世界标准人口构成作为标准。［结果］ 2009—2015年湖南省24个肿瘤登记地区合计年中人口数为40 618 446人年，共收集新发恶性淋巴瘤病例2515例，恶性淋巴瘤死亡病例1554例。所有恶性淋巴瘤病例病理组织学诊断比例99.36%，只有死亡医学证明书比例0.64%，死亡/发病比为0.62。全省恶性淋巴瘤发病率6.19/10万，中标发病率为4.65/10万，世标发病率4.53/10万，0～74岁累积率为0.78%，35~64岁截缩发病率为7.74/10万。城区居民恶性淋巴瘤发病率高于农村，男性恶性淋巴瘤发病率高于女性，男性恶性淋巴瘤发病顺位居男性全部恶性肿瘤发病的第7位，女性居第9位。全省居民恶性淋巴瘤死亡率为3.83/10万，中标死亡率为2.71/10万，世标死亡率2.64/10万，0～74 岁累积率为0.56%，35~64岁截缩死亡率为4.21/10万。城市居民恶性淋巴瘤死亡率高于农村居民，男性高于女性，男性恶性淋巴瘤死亡顺位居男性全部恶性肿瘤死亡的第7位，女性居第9位。［结论］ 恶性淋巴瘤是威胁湖南省居民身心健康的恶性肿瘤之一，应对高风险人群做好积极的防治工作。     

Expression and significance of HIF-1α and ET-1 in gastrointestinal stromal tumors

Objective To explore the expression and the value of HIF-1α,and ET-1 in judging the prognosis of gastrointestinal stromal tumors (GISTs). Methods The expression of HIF-1α, and ET-1 protein was examined in 76 GISTs by immunohistochemistry S -P methods. Results There was a positive correlation between the expression of HIF-1 α and ET-1 ( P < 0.05 ). The positive expression rate of HIF-1 α and ET-1 was 73.68% (50/76) ,and 65.79% (50/76) respectively,which was related with histologicial grade, tumor diameter, infiltration and metastasis, nuclear division rating of GISTs ( P < 0.05 ), but had no relationship with patients' age, gender, initial position of the tumor ( P > 0.05 ). There was statistically sig-nificant difference in the expression of HIF-1 α and ET-1 in the following groups:among the three classes of very low-risk and low-risk, middle-risk, high-risk, bewteen the diameter < 2 cm and > 5 cm (P < 0.05). The more malignant degree and larger diameter, the more highly positive expression rate ( P < 0.05 ). The positive expression in the groups with infiltration and metastasis, and nuclear division ≥5/50 HP was sig-nificantly higher than the groups without infiltration and metastasis, and nuclear division < 5/50 HP (P < 0.05). Conclusion The expression of HIF-1α had a significant correlation with ET-1. HIF-1α,and ET-1 expression was closely related with the prognosis of GISTs,and can serve as important predictors for survival.