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1.
采用聚乙烯硫酸(PVS)沉淀法测定血清低密度脂蛋白胆固醇(LDL-C)值,同时应用Friedewald公式计算LDL-C含量,并对两法结果进行比较分析,提出当甘油三脂(TG)<4.52mmol/L时,F公式计算法可代替PVS法,当TG>4.52mmol/L时,不宜用F公式计算LDL-C的值。 相似文献
2.
以辣根过氧化物酶(PO)和抗-PO作为免疫沉淀中的抗原和抗体,用光电比色法,对78例小儿肾脏疾病血清补体对免疫沉淀的抑制作用(IIPC)进行了研究,并同时检测补体成分C3、C4。结果,正常对照IIPCOD值为0.505±0.085,急性肾小球肾炎(0.137±0.108)显著降低(P<0.001);慢性肾小球肾炎(0.470±0.053)改变不明显(P>0.05);肾病综合征(0.401±0.038)明显低下(P<0.05)。IIPC低下的发生率依次为急性肾小球肾炎(83%)、肾病综合征(43%)、慢性肾小球肾炎(32%)。表明小儿肾小球疾病时IIPC大多降低并与疾病的活动性有关。因此认为IIPC低下在肾脏病的发生和发展中起一定作用。 相似文献
3.
冷春玲 《中国脊柱脊髓杂志》2007,(2)
为了确定盐析法提取特异性卵黄免疫球蛋白的最佳盐浓度,采用SDS—PAGE鉴定IgY的纯度,用RID测定IgY含量,间接ELISA法检测特异性卵黄抗体的活性。结果表明:用饱和度50%的硫酸铵盐析一次,再用140g/L的硫酸钠溶液盐析,可以获得469,6的回收率和77%的纯度。盐析液经超滤膜包超滤后制得的冷冻干燥制品,保持了完整的抗体结构和良好的抗体活性。这一结果为IgY的进一步分离纯化奠定了基础。 相似文献
4.
Summary Techniques and protocols are described for the generation of genetically modified cells that can be used for gene therapy. Primary fibroblast cultures are established from skin biopsies, maintained in culture, frozen for long-term storage, and retrieved when necessary. Retroviral packaging cell lines are generated by transfection of DNA into retroviral packaging cells by calcium-phosphate precipitation method or by lipofection method. To generate cell lines expressing high titer virus, individual colonies of cells are cloned and the virus titer is determined. Virus collected from packaging cells expressing high titer virus is then used to infect primary fibroblasts. To obtain fibroblast cell lines expressing high amounts of transgenes, individual cells can be cloned to generate clonal cell lines. Although the methods described here are for fibroblasts, the same methods or modification of the methods can be used for other cell types. 相似文献
5.
M. Suckfüll C. Wimmer B. Jäger K. Schorn J. Thiery 《European archives of oto-rhino-laryngology》2000,257(2):59-61
The pathogenesis of sudden hearing loss (SHL) is still not well understood. Possible causes include increased blood viscosity,
microthrombosis or altered blood flow. Hypercholesterolemia, hyperfibrinogenemia and increased platelet aggregation are frequently
observed in patients with SHL. The aim of this study was to investigate whether drastic lowering of plasma cholesterol and
fibrinogen by selective extracorporal apheresis could have a beneficial effect on hearing recovery in these patients. Seven
patients with SHL were treated with an extracorporal procedure removing fibrinogen and cholesterol from plasma. Six of the
seven patients showed an immediate improvement in auditory thresholds following a single treatment of heparin-induced low-density
lipoprotein precipitation. These findings indicate for the first time that acute and drastic removal of plasma fibrinogen
and low-density lipoproteins may be an effective clinical method for the treatment of patients with SHL.
Received: 19 February 1999 / Accepted: 6 May 1999 相似文献
6.
用日本第一化学药品株式会社LDL—C试剂盒在BeckmanCX4型自动生化分析仪上直接测定血清LDL—C含量。方法批内CV 0 37%~ 1 11% ,批间CV为 2 11%~ 3 0 2 % (n =2 0 )。平均回收率为 98 9% ,在 8 10mmol/L浓度范围内呈良好线性关系。TG <5 0 0mmol/L时与聚乙烯硫酸沉淀法 (X1)及Friedewald(X2 )公式计算结果相关良好 ,回归方程分别为Y =0 988X1+0 0 47,r=0 95 1,Y =0 96 1X2 +0 0 5 2 ,r=0 930。LDL—C直接测定法简单、快速 ,用血量少 ,勿需沉淀分离 ,适宜于自动化分析。 相似文献
7.
8.
建立了检测血清中异丙酚的反相高效液相色谱-荧光法.血清样品经含内标百里酚的丙酮溶液直接沉淀后,采用KR100-5 C18柱,甲醇-0.1%三氟醋酸溶液(80:20)为流动相,荧光激发波长为276nm,发射波长为307nm.异丙酚在0.05~12.8μg/ml范围内与峰面积比线性关系良好.检测限为150pg,异丙酚和百里酚的提取回收率均大于90%,批内、批间精密度为1.11%~6.88%. 相似文献
9.
10.
In the current study, the creep properties of magnesium alloy reinforced with SiC particles were investigated. For this purpose, ZK60/SiCp composite was produced by the stir casting method following the KoBo extrusion and precipitation hardening processes. The creep tests were performed at 150 °C under 10–110 MPa. The results showed that the stress exponent (n) and the average true activation energy (Q) was changed at high stresses, was found with increasing stress, the creep mechanism changing from grain boundary sliding to dislocation climb. The results of microstructure characterization after the creep test showed that at low stresses, the dynamic recrystallization resulting from twinning induced the GBS mechanism. However, at high stresses, with increasing diffusion rates, conditions are provided for dynamic precipitation and the dislocation climb of the dominant creep mechanism. Examination of the fracture surfaces and the surrounding areas showed that the cavity nucleation in the ternary boundary and surrounding precipitation was the main cause of damage. The evaluation of the samples texture after creep showed that the unreinforced alloy showed a moderately strong fiber texture along the angle of ϕ1 = 0–90°, which was tilted about Φ = 10°. A new strong texture component was observed at (90°, 5°, 0°) for the composite sample, which crept due to minor splitting of the basal pole by ~5° toward RD. 相似文献