Aloin improves TGF-β induced renal tubular epithelial cell fibrosis by activating Nrf2北大核心CSCD

Aim To investigate the effect of aloin, an aloe extract,on fibrosis of renal tubular epithelial cells (HK-2) induced by TGF-β and the underlying molecular mechanism. Methods The experiment included a control group,TGF-β induced group,TGF-β + Aloin 50 or 100 μmol • L-1group. The effect of aloin on cell viability was detected by CCK-8. The expression of fibrosis and antioxidant-related proteins was analyzed by Western blot and immunofluorescence. The ROS was detected by flow cytometry. The related indexes of oxidative stress were detected by SOD activity kit and MDA lipid oxidation kit. The inflammatory factors were detected by ELISA. Results Aloin did not affect the viability of HK-2 cells. Aloin decreased the expression of α-SMA, E-Cadherin, collagen I and fibronectin in HK-2 cells induced by TGF-β. Aloin up-regulated the expression of antioxidant molecules of Nrf2, HO-1 and NQOl, but reduced the levels of ROS and MDA, and restored the activity of SOD. Meanwhile, aloin also inhibited the release of IL-1β and IL-6 inflammatory factors. Conclusions Aloin can improve the oxidative stress and fibrogenesis of HK-2 cells induced by TGF-β through up-regulating Nrf2, and inhibit releasing inflammatory factors. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Controlled Aloin Release from Crosslinked Polyacrylamide Hydrogels: Effects of Mesh Size,Electric Field Strength and a Conductive Polymer

The aim of this paper is to investigate the effects of hydrogel mesh size, a conductive polymer, and electric field strength on controlled drug delivery phenomena using drug-loaded polyacrylamide hydrogels prepared at various crosslinking ratios both with and without a conductive polymer system. Poly(p-phenylene vinylene), PPV, as the model conductive polymer, was used to study its ability to control aloin released from aloin-doped poly(p-phenylene vinylene)/polyacrylamide hydrogel (aloin-doped PPV/PAAM). In the passive release, the diffusion of aloin from five aloin-doped PPV/PAAM hydrogel systems each was delayed ranging from during the first three hours to during the first 14 h due to the ionic interaction between the anionic drug and PPV. After the delayed periods, aloin could diffuse continuously into the buffer solution through the PAAM matrix. The amount of aloin released from the aloin-doped PPV/PAAM rose with increasing electric field strength as a result of the three mechanisms: the expansion of PPV chains inside the hydrogel, iontophoresis, and the electroporation of the matrix pore size, combined. Furthermore, the conductive polymer and the electric field could be used in combination to regulate the amount of release drug to a desired level, to control the release rate, and to switch the drug delivery on/off.     

HPLC法测定保健食品中芦荟苷含量不确定度的评定

目的:对高效液相色谱法测定保健食品中芦荟苷含量不确定度进行评定。方法:以便必舒牌芦荟胶囊为例,用HPLC法测定芦荟苷的含量,分析了整个测定过程产生的不确定度。结果:实验给出扩展不确定度以及最终结果表示:(60.49±2.24)mg.g-1。结论:分析产生不确定度的主要来源,为有效地控制用该方法来测定芦荟苷的含量提供可靠的理论依据。     

双波长分光光度法测定芦荟苷的含量

目的 :应用等吸收点双波长分光光度法对芦荟中芦荟苷含量测定进行研究。方法 :依据芦荟苷与芦荟大黄素的光谱特征 ,选择 36 1.4nm为测定波长 ,480 .9nm为参比波长。结果 :芦荟苷线性范围 1× 10 -6～ 1× 10 -4 mol/L ,回收率为 97.4%～10 1.6 % ,RSD为 0 .5 5 %～ 0 .74% ,芦荟大黄素对测定不干扰。结论 :本方法简便可靠 ,重现性好 ,为芦荟资源的进一步开发利用及质量控制等提供新的方法和重要依据     

芦荟素通过激活ERK1/2-Runx 2信号通路介导对去卵巢大鼠骨量流失的保护作用

目的观察芦荟素对去卵巢大鼠骨量的影响,并探讨可能的机制。方法将30只雌性Sprague Dawley大鼠随机分为3组:假手术组(Sham)、去卵巢组(OVX)及去卵巢大鼠+芦荟素治疗组(LHS,去卵巢大鼠每天接受50 mg/kg芦荟素治疗,持续12周)。12周后取双侧股骨进行微型计算机断层扫描(Micro-CT)检测股骨干骺端微观结果,通过组织病理切片观察骨小梁结果变化,通过骨生物力学检测骨强度改变以及运用蛋白质印迹(WB)检测可能的机制。结果OVX组大鼠的骨密度(bone mineral density,BMD)、骨显微结构、最大载荷和弹性模量等指标均明显低于Sham组(P<0.05)。芦荟素治疗后大鼠的骨密度、骨显微结构、最大载荷和弹性模量等指标均有明显改善,比较差异有统计学意义(P<0.05)。WB检测显示OVX组p-Erk1/2、Erk1/2、ALP、RUNX 2、OCN和OPN表达水平较Sham组明显下调(P<0.05)。LHS组p-Erk1/2、Erk1/2、ALP、RUNX 2、OCN和OPN表达水平较OVX组明显上调。结论芦荟素可以显著改善去卵巢大鼠股骨骨强度和骨量,这种疗效可能通过激活ERK1/2-Runx 2信号通路促进成骨来实现的。     

高效液相色谱法测定进口芦荟药材中芦荟苷的含量

目的 :建立了测定进口药材芦荟中芦荟苷含量的高效液相色谱法。方法 :采用高效液相色谱法 ,以ZORBAXSB-C₁₈(4.6mm× 2 50mm ,5μm)为色谱柱 ,流动相乙腈 水 (25∶75) ,检测波长 355nm ,流速 1.0mL·min^-1 。结果 :芦荟苷在 0.17～5.9μg线性关系良好 ,r=0.9999,芦荟苷平均加样回收率为 98.6% ,RSD 1.32 %(n =6)。结论 :试验结果表明 ,该方法准确、灵敏度高 ,重现性好。     

芦荟抗菌作用与蒽醌化合物的关系

目的 :分析芦荟抗菌作用与蒽醌化合物含量的关系 ,比较芦荟大黄素和芦荟苷的抗菌作用 ,探讨糖基取代结构对芦荟苷抗菌活性的影响。方法 :利用琼脂扩散法分析皂质芦荟叶皮提取物、芦荟大黄素和芦荟苷对 3种革兰氏阴性菌、2种革兰氏阳性菌的抑菌活性 ,利用扫描电镜技术分析了芦荟苷对大肠杆菌的作用。结果 :芦荟的抗菌作用与蒽醌化合物的含量呈正相关性。芦荟苷 (1g·L^-1)具有较强的抗菌活性 [抑菌直径大于 (7.1±0.15)mm] ,对革兰氏阳性菌的抗性高于革兰氏阴性菌。芦荟大黄素的抗菌活性较弱。芦荟苷能够改变细胞的形态 ,破坏细胞结构。结论 :蒽醌化合物是芦荟中的抗菌成分 ,芦荟苷是主要抗菌有效成分之一 ,葡萄糖基取代结构增强了其侵入细胞外被和杀菌的能力。     

通阻颗粒质量标准研究

目的:建立通阻颗粒质量标准.方法:采用薄层鉴别方法进行定性鉴别,运用高效液相色谱法测定芦荟苷含量.结果:确立了制剂中芦荟、甘草、诃子肉鉴别方法,通过方法学系统考察,建立了芦荟苷的含量测定方法,其芦荟苷的线性范围为0.41 ～2.46 μg,回归方程y=12 704X +48.84(r=0.999 9),平均加样回收率96.39％,RSD0.76％ (n=9).结论:建立的鉴别和含量测定方法简便、准确、重复性好,适用于质量控制.     

Bio‐prospecting endemic Mascarene Aloes for potential neuroprotectants

The Mascarene Aloes are used in the traditional pharmacopoeia against various ailments including cutaneous diseases and as antispasmodics. Scientific evidence to support these claims is non‐existent and mainly based on the scientific repute of A. vera. The antioxidant profile of methanolic leaf extracts of A. purpurea Lam., A. tormentorii (Marais) L. E. Newton & G. D. Rowley, A. lomatophylloides Balf. f., A. macra Haw. and A. vera (L.) Burm. f. was studied using the total antioxidant capacity, copper equivalent and superoxide dismutase assays. In vitro cytotoxicity was evaluated on CAD (Cath.‐a‐differentiated) neuronal cells by the methyl tetrazolium assay, and the neuroprotective profile was assessed using hydrogen peroxide‐induced neurotoxicity with the CAD cells. The aloin and vitexin content were determined by high‐performance liquid chromatography with diode‐array detection. A. purpurea had the highest aloin content (546.6 nmol/g), while A. tormentorii had the highest vitexin content (67.3 nmol/g). A. macra (concentration <0.1 mg/mL) elicited a 10% cytotoxicity effect on CAD cells while other Mascarene Aloes were not cytotoxic. This study validates the antioxidant and neuroprotective potential of Mascarene Aloes focusing on their aloin and vitexin content that are also present in other reputed medicinal Aloes.     

高效液相色谱法测定复方芦荟胶囊中芦荟苷的含量

目的 对复方芦荟胶囊中指标成分芦荟苷的含量测定方法进行研究.方法 采用反相高效液相色谱法,色谱柱:Polris C18(250 mm×4.6 mm,5μm)0DS柱,流动相:水-甲醇(58:42),流速:1.0 ml/min;检测波长:359 nm;柱温:室温.结果 芦荟苷在0.10～0.70μg范围内呈良好的线性关系...