首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   3篇
  免费   1篇
  国内免费   1篇
综合类   1篇
药学   3篇
中国医学   1篇
  2023年   1篇
  2010年   2篇
  2000年   1篇
  1998年   1篇
排序方式: 共有5条查询结果,搜索用时 15 毫秒
1
1.
脑靶向非病毒基因递释系统的研究进展   总被引:1,自引:0,他引:1  
脑靶向非病毒基因递释系统可有效介导基因药物跨越血脑屏障,到达病变部位发挥疗效,已成为研究热点之一。多项研究结果显示,通过适当机制如配体-受体特异性结合作用可显著提高非病毒基因递释系统在脑部的蓄积量,从而提高所携带外源基因在脑部的表达量。本文主要从受体介导和吸附介导两种机制入手,综述脑靶向非病毒基因递释系统的最新研究进展。  相似文献   
2.
脑靶向非病毒基因递释系统可有效介导基因药物跨越血脑屏障,到达病变部位发挥疗效,已成为研究热点之一。多项研究结果显示,通过适当机制如配体-受体特异性结合作用可显著提高非病毒基因递释系统在脑部的蓄积量,从而提高所携带外源基因在脑部的表达量。本文主要从受体介导和吸附介导两种机制入手,综述脑靶向非病毒基因递释系统的最新研究进展。  相似文献   
3.
4.
Purpose. This study was carried out to examine the blood-brain barrier(BBB) transport of human basic fibroblast growth factor (bFGF) andinvestigate its mechanism. Methods. The BBB transport of 125I-bFGF was measured by severalin vivo methods including intravenous administration, in situ internalcarotid artery perfusion, and intracerebral microinjection. The in vitrobinding of 125I-bFGF was characterized using freshly prepared bovinebrain capillaries. Results. The distribution volume of 125I-bFGF in the postvascularsupernatant increased with the perfusion time, and exceeded the spaceoccupied by the brain microvasculature and its trichloroacetic acid(TCA) precipitability was more than 90%. 125I-bFGF avidly bound toisolated bovine brain capillaries with a Bmax of 206 ± 48 pmol/mgprotein, and a Kd of 36.5 ± 15.7 nM. This binding was significantlyinhibited by unlabeled bFGF and heparin in a concentration-dependentmanner. The cationic peptides, protamine and poly-L-lysine (each 300M), produced over 85% inhibition of 125I-bFGF binding to braincapillaries. Furthermore, glycosaminoglycans with a sulfate residue,chondroitin sulfate B and C (each 10 g/mL) also inhibited the bindingof 125I-bFGF. The in vivo transcytosis of 125I-bFGF from the luminalside to the brain was also inhibited by the presence of heparin (10g/mL) and poly-L-lysine (300 M), whereas neither hyaruronic acid (10g/mL) nor insulin (10 M) had any effect. In addition to these results,the brain efflux index method was used to confirm that the transcytosisof 125I-bFGF from brain to blood across the BBB was negligible. Conclusions. These results suggest that 125I-bFGF is transported acrossthe BBB, possibly by an adsorptive-mediated transcytosis mechanismthat is triggered by binding to negatively charged species on the luminalmembrane surface of the brain microvasculature, such as heparansulfate proteoglycans.  相似文献   
5.
血脑屏障是中枢神经系统的一层特殊屏障,调节中枢神经系统稳态,允许正常的神经元功能,保护中枢神经系统免遭毒素、病原体和炎症的侵害。然而,脑毛细血管内皮细胞紧密连接的特性在保护的同时也极大程度限制了药物分子向内的递送效果。近年来,纳米制剂在中药靶向递药领域被广泛应用,较大的系统结构受制于血脑屏障导致疗效并不理想。基于血脑屏障上各种内源性受体及转运蛋白的发现,以及血脑屏障表面荷负电的生理特征,构建安全高效的靶向递药系统,从而设计受体介导、载体介导及吸附介导的转运途径实现血脑屏障的跨越,使药物制剂富集于脑部病变组织,有效提高疾病的治疗效果。受芳香中药“通关开窍”的传统功效启发,阐述其调节血脑屏障通透性的机制,并将这一功效与新兴纳米制剂结合,发挥中药特色进而实现制剂透过血脑屏障入脑释药。  相似文献   
1
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号