药用植物诃子ISSR-PCR反应体系的建立与优化

目的 建立和优化药用植物诃子的ISSR-PCR反应体系.方法 以诃子叶片为实验材料,采用CTAB法提取诃子DNA为模板,通过单因素和双因素实验,研究诃子ISSR-PCR反应体系中DNA模板、Taq DNA聚合酶、Mg2+、引物、dNTPs等的用量,以及退火温度对扩增结果的影响.结果 建立了适合诃子ISSR分析的反应体系...     

Cytoprotective effect on oxidative stress and inhibitory effect on cellular aging of Terminalia chebula fruit

The ethanol extract from the fruit of Terminalia chebula (Combretaceae) exhibited significant inhibitory activity on oxidative stress and the age-dependent shortening of the telomeric DNA length. In the peroxidation model using t-BuOOH, the T. chebula extract showed a notable cytoprotective effect on the HEK-N/F cells with 60.5 +/- 3.8% at a concentration of 50 microg/ml. In addition, the T. chebula extract exhibited a significant cytoprotective effect against UVB-induced oxidative damage. The life-span of the HEK-N/F cells was elongated by 40% as a result of the continuous administration of 3 microg/ml of the T. chebula extract compared to that of the control. These observations were attributed to the inhibitory effect of the T. chebula extract on the age-dependent shortening of the telomere, length as shown by the Southern blots of the terminal restriction fragments (TRFs) of DNA extracted from subculture passages.     

诃子提取物含药血清对乳鼠心肌细胞损伤的保护作用

目的:研究诃子提取物含药血清对H2O2所致心肌细胞损伤的保护作用.方法:采用H2O2处理培养心肌细胞,造成氧化应激模型,通过检测细胞培养液中LDH、CK漏出量、MDA含量和SOD活性反映诃子提取物含药血清对H2O2氧化模型的影响.结果:与模型组比较,诃子提取物含药血清能显著减少LDH、CK漏出量(P＜0.05,P＜0.01);降低培养液中MDA含量(P＜0.05,P＜0.01),升高SOD活性(P＜0.01).结论:诃子提取物含药血清可减轻H2O2对心肌细胞的损伤程度,对心肌细胞具有保护作用,其机制可能与稳定细胞膜和抗氧化有关.     

蒙药诃子中多糖的提取及含量测定

目的：从蒙药诃子中提取多糖,并测定其含量。方法：采用紫外分光光度法,以葡萄糖为对照品,用苯酚-硫酸显色法测定诃子中多糖的含量。结果：经方法学研究,多糖在3.212-19.27μg·mL-1范围内呈线性关系,回归方程Y=0.0432X ＋0.0146（r=0.9996,n=6）,含量为15.56%,平均回收率为100.4%,RSD=1.60%。结论：采用紫外可见分光光度法测定蒙药诃子中多糖含量的方法简便、快速、灵敏,结果准确可靠,适用于诃子中多糖含量的测定方法。     

Anti-arthritic and disease modifying activity of Terminalia chebula Retz. in experimental models

Objective This study evaluates the anti‐arthritic effect of Terminalia chebula hydroalcoholic extract (TCHE) in experimental models and attempts to correlate the effect of treatment on macrophage‐derived pro‐inflammatory cytokine expression and extent of disease activity. Methods Arthritis was induced in rats by subplantar administration of either formaldehyde or complete Freund's adjuvant (CFA). Joint size was measured at regular intervals by using a micrometer screw gauge. Serum and ankle joints of rats immunized with CFA were collected and subjected to ELISA for estimation of TNF‐α level and immuno‐histochemistry for detection of IL‐1β, IL‐6 and TNF‐R1, respectively. An acute and 28‐day oral toxicity study was carried out to evaluate the safety of the test drug. Key findings TCHE produced a significant inhibition of joint swelling as compared with control in both formaldehyde‐induced and CFA‐induced arthritis. TCHE treatment also reduced serum TNF‐α level and synovial expression of TNF‐R1, IL‐6 and IL‐1β. Results of acute toxicity study showed that the oral LD50 of TCHE was >2000 mg/kg. Chronic administration also did not produce any significant physiological changes as compared with normal rats. Conclusion Results indicate that the anti‐arthritic activity of TCHE was at least in part due to its modulatory effect on pro‐inflammatory cytokine expression in the synovium. We believe that TCHE has the potential to be used as a disease‐modifying agent in treatment of rheumatoid arthritis.     

藏药十味消食散中石榴子、诃子的TLC鉴别及桂皮醛、胡椒碱的HPLC测定

目的：建立藏药十味消食散的质量控制方法。方法：采用薄层色谱法进行定性鉴别,采用HPLC方法测定桂皮醛和胡椒碱的含量,色谱柱为Phenomenex C₁₈ 柱（250 mm×4.6 mm,5 μm）。结果：TLC法可鉴别石榴子、诃子的特征斑点;桂皮醛、胡椒碱的进样量分别在0.06-0.37 μg、0.05-0.33 μg 范围内呈良好的线性关系,回归方程分别为Y=9.273 2×10⁶X-2.348 2×10⁵,r=0.999 8;Y=7.315×10³X-3.857,r=1.000 0。结论：鉴别方法专属性强,定量方法准确,可操作性强,可对十味消食散的质量控制提供参考。     

诃子与草乌不同比例配伍对3种双酯型生物碱含量的影响

目的 建立草乌中主要毒性成分3种双酯型生物碱的HPLC含量测定方法,并测定其含量,探讨诃子-草乌不同比例配伍对3种双酯型生物碱含量的影响,阐释配伍减毒的原理.方法 采用HPLC含量测定方法对3种双酯型生物碱进行含量测定,色谱条件为:采用Extend C18柱(4.6 mm×250 mm,5μm),以甲醇-0.05％氨水流动相梯度洗脱,柱温30℃,检测波长235 nm,流速1 mL/min.结果 诃子-草乌不同比例(草乌单煎、0.5∶1、1∶1、2∶1、3.3∶1、5∶1)配伍合煎后,3种双酯型生物碱含量变化趋势略有不同.在水煎液中,3种双酯型生物碱总含量表现出先升高后降低的抛物线状趋势.在诃子-草乌(2∶1)合煎液中3种双酯型生物碱总含量最大,在诃子-草乌(5∶1)合煎液中3种双酯型生物碱总含量最低且低于单煎液.结论 诃子-草乌不同比例配伍对水煎液中3种双酯型生物碱的含量具有很大影响,一方面促进3种双酯型生物碱的溶出,另一方面在诃子配伍比例较高时,又加速了生物碱的水解.     

Screening South Indian medicinal plants for antifungal activity against cutaneous pathogens

In this study, twenty-eight South Indian medicinal plants were screened for their anti-fungal activity against six species of fungi (Trichophyton mentagrophytes, T. rubrum, T. soudanense, Candida albicans, Torulopsis glabrata, and C. krusei). Three plant species extracts, Celastrus paniculatus, Eriodendron anfractuosum and Ficus glomerata showed inhibitory activity. An aqueous extract of galls of Terminalia chebula showed inhibitory effects on three dermatophytes (Trichophyton spp.) and three yeasts (Candida spp.). Seeds extract of T. chebula inhibited only the growth of T. glabrata. An aqueous extract of T. chebula showed inhibitory effects higher than those measured in ethanol extracts. It is therefore suggested that tannins are plausible candidates for the anti-dermatophytic effects of T. chebula. Chebulinic acid, a known tannin of T. chebula was tested and found not inhibitory, thus a search for the active compound is needed.     

诃子叶与诃子硬种皮中丹宁反相高效液相色谱分析

用反相高效液相色谱（ＲＰ－ＨＰＬＣ）法对诃子叶、诃子硬种皮中的丹宁成分进行了分析。结果表明二者丹宁含量均较低,其中叶子中诃黎勒酸的含量为１．５６％,具有一定的开发利用价值。     

藏药十味乳香胶囊质量标准研究

目的:提高十味乳香胶囊质量标准。方法:采用薄层色谱法对处方中诃子和毛诃子进行定性鉴别;采用高效液相色谱法测定大黄酚的含量,使用FNG Redclassical C_(18)柱(250 mm×4.6 mm,5μm);以甲醇-0.1%磷酸溶液(80∶20)为流动相;流速为1.0 m L·min~(-1);检测波长为254 nm。结果:薄层色谱斑点清晰,阴性无干扰。大黄酚在0.100 8~1.008μg有良好的线性关系(r=0.999 7),平均加样回收率为99.02%(n=6),RSD为1.91%。结论:建立的方法准确、可靠、重复性好,可用于该制剂的质量控制。