GC法测定八角枝叶中反式茴香脑的含量

目的：建立八角茴香枝叶中反式茴香脑的含量测定方法。方法：采用气相色谱法,HP—INNOWax毛细管柱（0．32mm×30m,0．50μm）,分流比10：1程序升温,FID检测器,环己酮为内标物,以内标法定量。结果：反式茴香脑在0．2037—2．0367μg范围内,峰面积与其浓度呈良好线性关系（r=0．9994）,平均回收率为98．67％,RSD为1．38％（n=6）。结论：该方法简便、准确、重复性好,可作为八角枝叶的质量控制方法。     

固定化离子液体吸附分离八角茴香中莽草酸

目的 研究固定化离子液体（SILs）对八角茴香提取液中莽草酸的吸附分离性能,并分离制备莽草酸。方法 采用静态与动态吸附-解吸方法,研究SILs对莽草酸的吸附平衡和吸附动力学性能,并采用Freundlich型和Langmuir型方程拟合了303、313、323 K温度下的吸附等温线。结果 SILs对莽草酸具有良好的吸附特性,吸附等温线与Freundlich型和Langmuir型方程拟合效果均较好,且以Freundlich型方程拟合更佳。采用SILs对八角茴香中莽草酸进行富集分离,富集率为80.34%。结论 该方法简便易行、富集率高,可用于八角茴香提取液（物）中莽草酸的富集分离。     

Anti-inflammatory,analgesic and antioxidant activities of 3,4-oxo-isopropylidene-shikimic acid

Context 3,4-Oxo-isopropylidene-shikimic acid (ISA) is an analog of shikimic acid (SA). SA is extracted from the dry fruit of Illicium verum Hook. f. (Magnoliaceae), which has been used for treating stomachaches, skin inflammation and rheumatic pain.

Objective To investigate the anti-inflammatory, analgesic and antioxidant activities of ISA.

Materials and methods Analgesic and anti-inflammatory activities of ISA were evaluated using writhing, hot plate, xylene-induced ear oedema, carrageenan-induced paw oedema and cotton pellets-induced granuloma test, meanwhile the prostaglandin E₂ (PGE₂) and malondialdehyde (MDA) levels were assessed in the oedema paw tissue. ISA (60, 120 and 240?mg/kg in mice model and 50, 120 and 200?mg/kg in rat model) was administered orally, 30?min before induction of inflammation/pain. Additionally, ISA was administered for 12 d in rats from the day of cotton pellet implantation. The active oxygen species scavenging potencies of ISA (10^?3–10^?5 M) were evaluated by the electron spin resonance spin-trapping technique.

Results ISA caused a reduction of inflammation induced by xylene (18.1–31.4%), carrageenan (7.8–51.0%) and cotton pellets (11.4–24.0%). Furthermore, ISA decreased the production of PGE₂ and MDA in the rat paw tissue by 1.0–15.6% and 6.3–27.6%, respectively. ISA also reduced pain induced by acetic acid (15.6–48.9%) and hot plate (10.5–28.5%). Finally, ISA exhibited moderate antioxidant activity by scavenging the superoxide radical and hydroxyl radical with IC₅₀ values of 0.214 and 0.450?μg/mL, respectively.

Discussion and conclusion Our findings confirmed the anti-inflammatory, analgesic and antioxidant activities of ISA.     

大八角枝叶中1个新的seco-prezizaane型倍半萜化合物

目的研究大八角Illicius majus枝叶的化学成分。方法采用硅胶、Sephadex LH-20柱色谱、快速制备色谱和制备HPLC等技术进行分离和纯化,通过HR-ESI-MS、1D-NMR、2D-NMR等波谱数据鉴定化合物的结构。通过MTT法评价了化合物的细胞毒活性。结果从大八角枝叶提取物中分离得到1个seco-prezizaane型倍半萜,命名为大八角酮(1)。化合物1对人结肠癌细胞HCT-8、人肝癌细胞Bel-7402、人胃癌细胞BGC-823以及人肺癌细胞A549的IC50值分别为42.3、48.5、51.1和39.9μmol/L。结论化合物1为1个新的倍半萜类化合物,未表现出明显的细胞毒活性。     

桂皮和茴香的诱变性研究

模拟一种习惯烹调方式，从桂皮、茴香中提取食用成份，分别对小鼠骨髓ＰＣＥ微核测试系统进行检测。结果表明用本方法提取的桂皮和茴香食用成份各剂量组的小鼠骨髓ＰＣＥ微核诱发率与阴性对照组的微核率（１．１‰）差异无显著意义（Ｐ＞０．５）。故认为按此习惯方式食用桂皮和茴香是安全的。     

三种调味品对小鼠内脏器官的影响

目的探讨过量八角、花椒及桂皮对小鼠内脏器官的影响。方法分别使用不同浓度三种调味品的提取液灌胃 ,采用病理学检验技术 ,观察小鼠肝脏、肾脏等器官的病理学变化 ,并计算出肝、肾、脾的脏体系数。结果调味品各组小鼠的肝脏、肾脏和脾脏重量都有所增加 ,脏体系数有不同程度升高 ,与正常组比较 ,差异具显著性 (P <0 .0 5 ,P <0 .0 1 )。肝脏、肾脏可见明显形态学改变 ,肝脏的损伤主要表现在肝细胞胞浆疏松化和气球样变 ,甚至出现双核、巨核和分裂相。肾脏损伤主要表现为肾小管上皮细胞水肿 ,肾小球毛细血管充血、出血。脾脏未见明显改变。结论三种调味品过量使用时 ,对小鼠肝脏、肾脏和脾脏有不同程度的损伤 ,但病变的程度与调味品的剂量关系不大 ,而与其种类关系较密切。八角的毒性反应最为明显 ,桂皮次之 ,花椒的影响较小     

Antimicrobial properties of star anise (Illicium verum Hook f)

Star anise (Illicium verum Hook f) has been shown to possess potent antimicrobial properties. Chemical studies indicate that a major portion of this antimicrobial property is due to anethole present in the dried fruit. Studies with isolated anethole (compared with standard anethole) indicated that it is effective against bacteria, yeast and fungal strains. Copyright © 2002 John Wiley & Sons, Ltd.     

少药八角果实中的黄酮类成分研究

目的分离并鉴定少药八角Illicium oligandrum果实中的黄酮类成分。方法利用硅胶柱色谱、凝胶(Sephadex LH-20)柱色谱和ODS反相柱色谱等方法进行分离和纯化,应用核磁共振和质谱等现代波谱技术进行结构鉴定。结果从少药八角果实的95%乙醇提取物中分离得到11个黄酮类化合物,分别鉴定为:山柰酚(Ⅰ)、槲皮素(Ⅱ)、槲皮素-3-O-β-D-半乳吡喃糖苷(Ⅲ)、异=鼠李素-3-O-β-D-葡萄吡喃糖苷(Ⅳ)、槲皮素-3-O-α-L-阿拉伯吡喃糖苷(Ⅴ)、槲皮素-3-O-α-L-鼠李吡喃糖苷(Ⅵ)、二氢槲皮素-3-O-α-L-鼠李吡喃糖苷(Ⅶ)、二氢山柰酚-3-O-α-L-鼠李吡喃糖苷(Ⅷ)、槲皮素-3-O-(6″-O-α-L-鼠李吡喃糖基)-β-D-葡萄吡喃糖苷(Ⅸ)、山柰酚-3-O-(6″-O-α-L-鼠李吡喃糖基)-β-D-葡萄吡喃糖苷(Ⅹ)、异鼠李素-3-O-(6″-O-α-L-鼠李吡喃糖基)-β-D-葡萄吡喃糖苷(Ⅺ)。结论11个化合物均为首次从该植物中分离得到,其中,化合物Ⅳ、Ⅴ、Ⅶ、Ⅷ、Ⅺ为首次从该属植物中分离得到。     

广西不同产地八角茴香中茴香脑的含量测定

用气相色谱方法测定了广西不同产地的八角茴香中茴香脑的含量.结果表明,广西不同产地八角茴香药材中茴香脑的含量均大于4.5%.