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Karin Stiasny Stefan Malafa Stephan W. Aberle Iris Medits Georgios Tsouchnikas Judith H. Aberle Heidemarie Holzmann Franz X. Heinz 《Viruses》2021,13(4)
Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviviruses. Therefore, serological diagnosis of a recent infection can be insufficiently specific, especially in areas where flaviviruses co-circulate and/or vaccination coverage against certain flaviviruses is high. In this study, we developed a new IgM assay format, which is well suited for the specific diagnosis of TBE, Zika and dengue virus infections. In the case of TBE and Zika, the IgM response proved to be highly specific for the infecting virus. In contrast, primary dengue virus infections induced substantial amounts of cross-reactive IgM antibodies, which is most likely explained by structural peculiarities of dengue virus particles. Despite the presence of cross-reactive IgM, the standardized nature and the quantitative read-out of the assay even allowed the serotype-specific diagnosis of recent dengue virus infections in most instances. 相似文献
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《Indian journal of medical microbiology》2019,37(2):278-280
Acute undifferentiated febrile illness (AUFI) constitutes the predominant cause of healthcare seeking in Odisha. This prospective study was conducted to analyse the clinical, epidemiological and laboratory profile of scrub typhus patients presenting with AUFI from January to December 2017. Four hundred and thirty-two samples were tested for dengue, malaria, scrub typhus and enteric fever. Scrub typhus was overall the most common cause of AUFI (26.3%, 114/432) followed by dengue (19.2%, 83/432). Eschar was seen in 6.1% of cases. Aetiologies of 38.6% of AUFI remained unidentified. In the present study, there was no mortality attributed to scrub typhus. 相似文献
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《Digestive and liver disease》2019,51(6):864-869
BackgroundWe aimed to clarify whether presence of hepatitis B virus (HBV) markers in cord blood indicates exposure to or infection with HBV.MethodsWe prospectively recruited HBsAg-positive pregnant women and their neonates 2012 through 2015. All neonates received postnatal immunoprophylaxis. The infants were followed up at 7–14 months of age.ResultsTotally 329 HBsAg-positive pregnant women and 333 neonates were enrolled. No cord blood was anti-HBc IgM positive. A total of 290 (87.1%) neonates were followed up at 7–14 months of age and 6 (2.1%) of them were infected with HBV. Of 146 neonates born to HBeAg-negative mothers, 38 (26.0%) and 30 (20.5%) had detectable HBsAg and HBV DNA in cord blood respectively, but none of 126 infants followed up was infected. Of 187 neonates born to HBeAg-positive mothers, 92 (49.2%) and 79 (42.2%) had detectable HBsAg and HBV DNA in cord blood respectively; 6 (3.7%) of 164 infants followed up were infected. Of seven neonates with HBV DNA > 105 IU/ml in cord blood, four had no infection and three others were infected.ConclusionPresence of HBsAg and/or HBV DNA, even at high levels, in cord blood just indicates exposure to, but not infection with HBV. Presence of HBV markers in cord blood cannot define intrauterine infection. 相似文献
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目的了解宁海地区0岁~14岁儿童八项呼吸道病原体IgM检测结果及流行情况,为儿童呼吸道疾病提供科学诊治依据。方法收集本院儿科2017年5月-2018年4月的0岁~14岁1 782例儿童血清,采用间接免疫荧光法检测流感病毒A(INFA)、流感病毒B(INFB)、呼吸道合胞病毒(RSV)等八项呼吸道病原体IgM抗体,分析不同性别、年龄和季节患儿感染情况。结果总阳性标本检出率为57.52%,前三位分别为INFB(41.75%)、MP(37.03%)、INFA(20.37%);不同年龄组IgM阳性率差异有统计学意义(χ^2=216.892,P<0.05),学龄组阳性率最高(78.31%);不同季节阳性检出率差异无统计学意义(χ^2=5.213,P>0.05);女童阳性率高于男童(χ^2=64.875,P<0.05)。结论 INFB、MP和INFA是宁海地区儿童最主要的呼吸道感染病原体,不同年龄段和性别存在显著差异,应针对性采用有效的措施预防感染。 相似文献
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目的:分析呼吸道感染患者病原体IgM 抗体、C反应蛋白(CRP)及白细胞(WBC)检测结果。方法纳入2013年1~12月因呼吸道感染就诊的患者1402例,采用间接免疫荧光方法进行9种呼吸道感染病原体Ig M 抗体检测,采用全自动分析仪进行CRP及WBC检测。结果 1402份血清标本中,检出呼吸道病原体IgM 抗体阳性标本633份,阳性率为45.15%。阳性率前3位病原体依次为肺炎支原体(13.91%)、乙型流感病毒(9.99%)和甲型流感病毒(6.42%)。在633例呼吸道病原体Ig M抗体阳性患者中,WBC>10×109/L者有125例(占19.75%) ,CRP>8.0 mg/L者有103例(占16.27%)。结论综合分析呼吸道病原体IgM抗体、WBC、CRP检测结果,对于判断感染病原体类型、病情严重程度,以及合理制订治疗方案有重要意义。 相似文献
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目的探讨呼吸道病原体九联检在小儿呼吸道感染中的临床应用价值。方法对2012年3月—2013年4月期间在该院住院的呼吸道感染的患儿(共104例)采用九项呼吸道感染病原体Ig M抗体检测试剂(间接免疫荧光法)同时检测九项病原体的Ig M抗体。结果在小儿呼吸道感染患者中,九种病原体检出的阳性率为57.69%,其中主要以肺炎支原体最为常见,占阳性例数的56.7%,其次为腺病毒40%、嗜肺军团菌23.3%;混合感染占感染比例的33.3%。结论呼吸道病原体九联检在小儿呼吸道感染检测中检出阳性率高,检测的病原体种类多,发现病原体时间早,对临床的及时诊断、治疗及用药有十分重要的意义。 相似文献
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Fengling Wang Xi Ye Yifan Wu Huihui Wang Chengming Sheng Daiyin Peng Weidong Chen 《Journal of pharmaceutical sciences》2019,108(1):641-651
Repeated injection of PEGylated liposomes can cause the disappearance of long circulating property because of the induction of anti-PEG IgM antibody referred to as “accelerated blood clearance (ABC) phenomenon.” Although ABC phenomenon typically occurs when entrapped drugs are chemotherapeutic agent with low cytotoxic, there is little evidence of accelerated blood clearance of PEGylated herbal-derived compound on repeated injection. Herein, we investigated the blood concentration of PEGylated liposomal gambogenic acid (PEG-GEA-L), a model PEGylated liposomal herbal extract, on its repeated injection to rats. We found time interval between injections had considerable impact on the magnitude of ABC phenomenon induced by PEG-GEA-L. When time interval was prolonged from 3 days to 7 days, ABC phenomenon could be attenuated. Furthermore, its magnitude was enhanced accompanied by a marked rise in the accumulation of PEG-GEA-L in the liver and spleen in a first-dose–dependent manner. Consistently, the level of anti-PEG IgM significantly increased with the first dose of PEG-GEA-L and decreased with the extended time interval between injections, which implies anti-PEG IgM is a major contributor to the ABC phenomenon. Notably, the increased expression of liver anti-PEG IgM was accompanied by an increased expression of efflux transporters in the induction process of the ABC phenomenon. 相似文献
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目的探讨疱疹病毒抗体阳性的肺炎支原体肺炎(MPP)患儿免疫状况及临床特征。方法回顾分析2015年1月—2017年5月收治的360例2个月~16岁因MPP住院的患儿临床资料,以EB病毒(EBV)、巨细胞病毒(CMV)、单纯疱疹病毒(HSV)任一疱疹病毒IgM抗体阳性的162例患儿为抗体阳性组,另198例EBV、CMV、HSV抗体均阴性的患儿作为对照组,两组进行分析比较。结果 360例MPP患儿中男188例、女172例,合并疱疹病毒IgM抗体阳性162例,其中EBV IgM阳性97例,阳性率26.9%;CMV IgM阳性39例,阳性率10.8%;HSV IgM阳性97例,阳性率26.9%。所有患儿均未予抗病毒治疗,经治疗症状缓解好转出院。抗体阳性组重症MPP、大叶性实变、并发呼吸衰竭的比例均高于对照组,血清IgM、CD~(4+)T细胞、CD~(8+)T细胞、淋巴细胞、NK细胞、白细胞计数、乳酸脱氢酶、丙氨酸氨基转移酶均高于对照组,大环内酯类抗生素使用时间长于对照组,使用激素率高于对照组,差异均有统计学意义(P均0.05)。MPP合并2种病毒IgM抗体阳性者的血IgM和三酰甘油水平高于合并1种者,差异有统计学意义(P0.05)。合并3种病毒IgM抗体阳性者与合并1种者比较,住院时间、发热时间、大环内酯类抗生素使用时间均有延长,血IgM、丙氨酸氨基转移酶水平更高,重症MPP发生率增高,差异均有统计学意义(P0.05)。结论疱疹病毒IgM抗体阳性MPP患儿的炎症反应强烈,临床症状重,病程长,并发症多,需更长时间的治疗。 相似文献