蛇六谷石油醚萃取物柱层析部位抗肿瘤活性组分再筛选

[目的]对中药蛇六谷石油醚萃取物采用硅胶柱层析后的所得组分进行体外抗肿瘤活性筛选。[方法]采用硅胶吸附层析分离技术,对蛇六谷石油醚萃取物的化学成分进行分离,应用MTT法检测各组分对HepG-2、SGC-7901及C6细胞增殖的影响。[结果]蛇六谷石油醚萃取物经硅胶柱层析分离后,得到7个组分;MTT法实验结果显示石A和石B组分对HepG-2、SGC-7901及C6细胞均有较明显的抑瘤作用,其中以石B组分抑瘤作用最强。[结论]蛇六谷石油醚萃取物经硅胶柱层析分离得7个组分中石B组分抑瘤作用最强。     

Effects of Petroleum Ether Extract of Amorphophallus paeoniifolius Tuber on Central Nervous System in Mice

The central nervous system activity of the petroleum ether extract of Amorphophallus paeoniifolius tuber was examined in mice, fed normal as well as healthy conditions. The petroleum ether extract of Amorphophallus paeoniifolius tuber at the doses of 100, 300 and 1000 mg/kg showed significant central nervous system activity in mice.     

魔芋对艾氏腹水瘤(EAC)抑制作用的研究

 目的　探讨魔芋对肿瘤的抑制作 用及对免疫功能的影响。方法　分别将魔芋精粉(RKM),魔芋灰粉、新鲜魔 芋茎块煎剂,环磷酰胺、生理盐水对艾氏腹水瘤(EAC)小鼠灌胃给药。结果　各实验组与盐水对照组相比有明显的抑瘤作用,能延长小鼠的生存时间,同时对动物白 细胞无影响,并能增加淋巴细胞转化率。结论　魔芋可能是通过增强机体免 疫功能来抑制移植性肿瘤的生长,起到抗肿瘤作用。     

魔芋药用研究( )议

对魔芋目前抗癌的研究现状提出了疑问,然后综述了魔芋的药理作用及其临床应用。     

Antioxidant potential of Amorphophallus paeoniifolius in relation to their phenolic content

The present study evaluates the antioxidative and radical scavenging potential of the tuber extract of Amorphophallus paeoniifolius (Dennst.) Nicolson, (Araceae). The ethanol extract of A. paeoniifolius (APE) was studied for the inhibition of lipid peroxidation estimated in terms of thiobarbituric acid reactive substances (TBARS) and the levels were reduced by 4.3% to 67.2% in a dose-dependent manner. Further, APE was analyzed for scavenging capacities based on 1,1-diphenyl-2-picrylhydrazyl-2-radical (DPPH) assay and percentage inhibition activity based on 2,2-azinobis-(-3-ethyl) benzo-thiozoline-6-sulfonate (ABTS⁺) and H₂O₂. The A. paeoniifolius extract showed a maximum of 68.6% of DPPH scavenging activity and the maximum inhibition of 74% and 67.2% in the case of ABTS and H₂O₂, respectively. The antioxidant efficiency and inhibition of oxidation of the extract was found to be dose-dependent at the tested concentrations of 1-50?µg/mL. High-performance thin layer liquid chromatography (HPTLC) profile of the extract suggests the presence of polyphenols such as gallic acid, resveratrol, quercetin and two unidentified compounds. The results suggest that the ethanol extract of A. paeoniifolius has a potent antioxidant activity in vitro and can be utilized as an effective and safe source of antioxidants.     

蛇六谷乙酸乙酯萃取物A1组分对人胃癌SGC-7901细胞增殖及bcl-2 Bax基因表达的影响

目的：探讨蛇六谷乙酸乙酯萃取物A1组分对人胃癌SGC-7901细胞株的增殖抑制作用及其作用机制。方法：用MTT法检测不同浓度A1组分对SGC-7901细胞的增殖抑制作用,光镜学观察A1组分对SGC-7901的细胞形态学变化,RT-PCR法观察A1组分对SGC-7901细胞bcl-2及Bax基因表达的影响。结果：A1组分对SGC-7901细胞增殖抑制作用为剂量依赖性,并具有明显的细胞形态学改变;RT-PCR结果显示,随着A1组分剂量的增加,具有明显的下调bcl-2基因及上调Bax基因水平的作用。结论：A1组分对人胃癌SGC-7901细胞的增殖具有明显的抑制作用,其抑制作用可能与下调bcl-2基因及上调Bax基因水平作用有关。     

Evaluation of the pharmacotherapeutic efficacy of Garcinia cambogia plus Amorphophallus konjac for the treatment of obesity

Hydroxycitric acid (HCA), the main compound of Garcinia cambogia extract, is a competitive blocker of ATP-citrate-lyase, presenting a potential inhibition of fatty acid biosynthesis. Glucomannan fibers, abundant in Amorphophallus konjac, seem to reduce the absorption kinetics of dietary fat. Therefore, the aim of this double-blind randomized study was to evaluate the pharmacotherapeutic efficacy of standardized extracts of G. cambogia (52.4% HCA) plus A. konjac (94.9% glucomannan) in the treatment of obesity. Fifty-eight obese subjects (BMI 30.0-39.9 kg/m(2)) were assigned to the placebo group (n = 26) or the treatment group (n = 32); no dietary restrictions were applied. Over a 12-week period, subjects were given daily doses of either Garcinia (2.4 g) plus Konjac (1.5 g) or placebo prior to their main meals (3 times/day). Before the start of treatment, and every 4 weeks thereafter, the following were recorded: height, weight, circumferences and body composition, resting energy expenditure (REE), lipid profile and glucose levels. The treatment had no significant effect on anthropometric parameters, REE, triglycerides or glucose levels. However, a significant reduction was observed in total cholesterol (-32.0 +/- 35.1 mg/dL) and LDL-c levels (-28.7 +/- 32.7 mg/dL) in the treated group, the final levels being significantly lower than those of the placebo group (p = 0.008 and p = 0.020, respectively). The results obtained suggest that the treatment had a significant hypocholesterolemic effect, without influencing the anthropometric or calorimetric parameters tested.     

魔芋属三种食疗植物中的营养成分分析

<正> 魔芋食用、药用历史悠久,据中国植物志和本草考证结果,其原植物应为花魔芋(A.konjac)和疏毛魔芋(A.sinensis)。但近年刘佩瑛又将生产“金河芋角”的魔芋的原植物定为一个新种,即白魔     

魔芋超细及纳米粉末的减肥特性研究

目的　考察魔芋超细及纳米粉末对营养性肥胖大鼠的减肥作用。方法　用紫外可见分光光度法测定大鼠血液中的甘油三酯、胆固醇和高密度脂蛋白含量 ,用分光光度法测定大鼠血糖值 ,通过测量体重变化及 L ee's系数考察魔芋超细及纳米粉末的减肥效果。结果　与魔芋精粉及魔芋多糖相比 ,魔芋超细及纳米粉末可明显降低营养性肥胖大鼠的体重和 L ee's系数 (P<0 .0 5 ) ,还可明显降低营养性肥胖大鼠血液中的甘油三酯、胆固醇和高密度脂蛋白含量 (P<0 .0 5 )。结论　魔芋超细及纳米粉末具有比魔芋精粉及魔芋多糖更明显的减肥作用。     

正交试验优选蛇六谷减毒炮制工艺

目的:优选蛇六谷减毒炮制工艺。方法:以蛇六谷口尝刺激性、兔眼刺激性综合评分为指标,在单因素试验的基础上设计正交试验,考察饱和氢氧化钙溶液用量、加热时间、加热温度对炮制效果的影响,并进行验证试验及炮制前后样品刺激性比较。结果:饱和氢氧化钙溶液用量、加热温度对炮制效果有显著影响;最优炮制工艺为100℃下加入30倍药材量的饱和氢氧化钙溶液加热30 min;验证试验综合评分分别为8.05、8.44、8.37(RSD=2.5%,n=3);炮制前、后综合评分平均值分别为0.12、8.54(n=10)。结论:与炮制前比较,药材经优选工艺炮制后刺激性降低;优选工艺稳定可靠。