IL-37b作用于树突状细胞CD39/ATP轴抑制类风湿性关节炎大鼠炎症反应的作用及机制

目的探讨白细胞介素37b重组蛋白(rmIL-37b)通过调节CD39/ATP轴抑制树突状细胞(DC)诱导类风湿性关节炎(RA)大鼠炎症反应的机制。方法将SD大鼠随机分为空白对照组(CTL)、CIA模型组、rmIL-37b 5μg/kg组、rmIL-37b 10μg/kg组,每组各10只。除了空白对照组外,其余大鼠采用含有卡介苗的完全弗氏佐剂和牛Ⅱ型胶原混合乳液免疫刺激,建立CIA模型。确定建模成功当天(D0),rmIL-37b组分别尾静脉注射5μg/kg、10μg/kg rmIL-37b;CTL组和CIA模型组注射相同体积的(1 ml/kg)生理盐水,连续给药15 d。免疫组化法检测滑膜组织Nod样受体蛋白3(NRPL3)炎症小体的表达,流式细胞术检测DC表型,另外试剂盒检测血清三磷酸腺苷(ATP)和免疫学指标。结果与CIA模型组相比,rmIL-37b 10μg/kg组大鼠足容积、AI值、NLRP3炎症小体表达量、血清ATP、IL-1β、IL-18、肿瘤坏死因子α(TNF-α)、抗Ⅱ型胶原抗体亚型(anti-ColⅡ-IgG、anti-ColⅡ-IgG2a)水平均降低,同时DC表面CD...     

三棱火针加拔罐治疗腘窝囊肿30例

ObjectiveTo explore the therapeutic effect of the fire needling with the three-edged needle and the cupping on popliteal cyst.MethodsA total of 60 patients of popliteal cyst were divided into two groups, 30 cases in each one according to the random number table. In the fire needling group, the burnt-red three-edged needle was inserted to the deep layer of the cyst. After the fluid was squeezed out, the cupping was exerted. The treatment was given 1 to 2 times a week, for 4 weeks totally. In the western medication group, prednisolone acetate 25 mg was injected in the cyst capsule, once a week, for 4 weeks totally. The effective rate, recurrence rate and Rauschning–Lindgren grading were evaluated before and after treatment.ResultsThe total effective rate was 80.0% in the fire needling group and was 83.3% in the western medication group, without statistical significance in comparison (P > 0.05). The recurrence rate was 6.7% in the fire needling group and was 30% in the western medication group. The recurrence rate in the fire needling group was lower than that in the western medication group, indicating the statistical significance (P < 0.05). After treatment, Rauschning–Lindgren grade was different statistically as compared with that before treatment in either group (both P < 0.05). Rauschning–Lindgren grade was distributed in 0 to I in the fire needling group after treatment, which was different significantly as compared with the western medication group (P < 0.05).ConclusionThe combined treatment with the fire needling combined with cupping therapy achieves the satisfactory effect on popliteal cyst and the very low recurrence rate and it is easy in operation and deserves to be promoted in primary care.     

刘晓鹰教授“扶中”外治法治疗儿科疾病临床经验浅析

小儿"脾常不足",脾胃之体成而未全,脾胃之气全而未壮,脾胃不足在儿科疾病发生发展、病机转归中占有相当重要的地位。刘晓鹰教授在继承全国名老中医倪珠英学术思想基础上,提出"扶中"外治法,立法取穴方面强调时时顾护小儿中焦脾胃,采用小儿推拿、扶阳罐(含温推、温灸和温刮痧)、针刺(含腹针、揿针)等外治手段治疗儿科脾、肺、肾等系列疾病,取得满意疗效。     

"双固一通"艾灸法对糖尿病及糖尿病周围神经病变大鼠海马BDNF和NT-3蛋白的影响

目的观察“双固一通”艾灸法对糖尿病DM 及糖尿病周围神经病变(DPN)大鼠血糖及海马中神经营养因子(BDNF)和神经营养素-3(NT-3)蛋白的影响。方法75只雄性SD大鼠随机分为A组、B组、C组、D组和E组,每组15只。A组为正常对照组,不进行任何干预,B组为DM模型组,造模后不行艾灸干预;C组为DM艾灸治疗组,采用艾条悬灸;D组为DPN模型组,造模后不行艾灸干预;E组为DPN艾灸治疗组,采用艾条悬灸。于造模后72 h、4周治疗结束后和8周治疗结束后空腹尾静脉采血测血糖,于4周治疗结束后和8周治疗结束后,用免疫组化试验方法检测各组大鼠海马中BDNF和NT-3蛋白表达情况。结果B组、D组与A组血糖水平比较,差异有统计学意义(P<0.05),C组与B组比较,大鼠血糖水平降低(P<0.05);E组与D组比较,大鼠血糖水平降低(P<0.05)。B组、D组大鼠海马BDNF及NT-3免疫阳性神经元平均光密度值较A组明显减少(P<0.05);与B组比较,C组大鼠BDNF及NT-3免疫阳性神经元平均光密度值明显增加(P<0.05);与D组比较,E组大鼠BDNF及NT-3免疫阳性神经元平均光密度值明显增加(P<0.05)。结论“双固一通”艾灸法不仅可以起到明显的降糖效果,还可通过影响BDNF和NT-3蛋白的产生,保护糖尿病大鼠的感觉神经元,并对糖尿病及并发症周围神经病变起到治疗作用。     

基于病因病机分析各省市新型冠状病毒肺炎的中医药防治

新型冠状病毒肺炎属于中医学“疫病”范畴。疫情爆发以来,全国各省市卫生行政管理部门坚持中西医结合原则,陆续制定了各省市的中医药防治方案,三因制宜,分期分型辨证并不断完善。笔者全面收集并整理相关信息,基于病因病机分析了各省市中医药防治方案,认为此次疫病是外感疫气,病位主要在肺卫,病邪主要表现为“毒、湿、热、虚、寒”。此“毒”即为疫气,武汉遇暖冬,非其时而有其气而发疫,一气一病,播散流行,治疫必先治毒;“湿”邪之地在南北方各占一半,东西部均有,在南方源于季节和地域,在北方可由寒挟杂,治疗均应祛湿;“热”主要见于南方,为南方常见的病邪,亦可因湿毒入里,湿郁化热,而在北方热与湿同现,主要是因湿毒入里,湿郁化热,治热均以清为主;“虚”则主要在北方,北方冬季多寒,易伤正致虚,或邪正交争激烈,伤阳伤阴致虚,宜祛邪为标,扶正为本;“寒”见于北方和南方山区,寒气过甚,易伤阳伤正,故尤其要注重预防,且以补正气为主。中医防疫治疫应三因制宜,重视预防,与时俱进。     

Emotional triangle and Yìntáng (印堂EX-HN3) for insomnia treatment – case report

Insomnia prevalence may reach up to 30% of the population considering at least one of the following criteria: difficulty in falling asleep, difficulty in remaining asleep, waking up too early, not repairing sleep or bad sleep quality, followed by daily consequences of lack of sleep such as lack of attention or stress. Several studies have reported the use of acupuncture as a therapeutic alternative for insomnia treatment showing important results such as anxiety decrease, improvement of sleep quality, decrease of daily consequences of insomnia, among others. The aim of this project was to use acupuncture as treatment for insomnia using the points Shénmén (神门HT7)， Tàiyuān (太渊LU9)， Nèiguān (内关PC6) and Yìntáng (印堂EX-HN3). After the signing of the TCLE, the patient answered a questionnaire during sessions 1, 5 and 10. The patient received insomnia treatment through puncture of the following points: HT7, LU9, PC6 and EX-HN3. The patient reported a significant improve in the sleep quality after the first sessions and absence of the symptoms after the tenth session. Therefore we conclude that the stimulation of these acupoints is useful in the insomnia treatment.     

经皮耳迷走神经刺激干预功能性消化不良模型大鼠的效应及机制研究

ObjectiveTo explore the effect and its possible mechanism of the intervention of transcutaneous auricular vagus nerve stimulation (taVNS) for the functional dyspepsia (FD) model rats.MethodsOf the 25 male SD rats, 6 rats were set as blank group, the other 19 rats were established to be the functional dyspepsia (FD) model by iodoacetamide intragastric administration, and 18 FD models were successfully established. The 18 model rats were randomly divided into a model group, a sham-taVNS group, and taVNS group, with 6 rats in each group. There was no intervention applied to the rats in the blank and model groups. Whereas, the rats in sham-taVNS group received stimulation to the rim of auricular concha of both sides, and those in taVNS group received stimulation to the cavity of auricular concha of both sides, for 30 min each time, once a day, 7 days in succession. After the intervention, the gastric sensitivity of the rats in each group under different pressure conditions in the stomach, the expressions of serum brain-gut peptide motilin (MTL), cholecystokinin (CCK), glucagon-likepeptide1 (GLP-1), and inflammatory factors IL-4, IL-10, and IL-1β were detected.Results(1) Gastric sensitivity: compared with the blank group, the gastric sensitivity of the model group was higher (P <0.05). Compared with the model group, the gastric sensitivity of the taVNS group was lower (P < 0.05). Compared with the sham-taVNS group, the gastric sensitivity of the taVNS group was lower (P <0.05).(2) Expression of brain-gut peptide: compared with the blank group, MTL was lower, CCK and GLP-1 were higher in the model group (all P <0.05). Compared with the model group, MTL was higher, CCK and GLP-1 were lower in the taVNS group (all P <0.05). Compared with the sham-taVNS group, CCK and GLP-1 were lower in the taVNS group (both P<0.05). (3)Expression of inflammatory factors: compared with the blank group, IL-4 and IL-10 were lower and IL-1β was higher in the model group (all P <0.05). Compared with the model group, IL-10 was higher and IL-1β was lower in the sham-taVNS group (all P <0.05), while IL-4 and IL-10 were higher and IL-1β was lower in the taVNS group (all P <0.05). Compared with the sham-taVNS group, IL-4 and IL-10 were higher and IL-1β was lower in the taVNS group (all P <0.05).ConclusionTaVNS can reduce the gastric sensitivity of FD model rats by peripheral anti-inflammatory action and regulating the abnormal secretion of brain-gut peptide.     

Isolates of Alpinia officinarum Hance as COX-2 inhibitors: Evidence from anti-inflammatory,antioxidant and molecular docking studies

BackgroundInflammation triggered by oxidative stress can cause various ailments, such as cancer, rheumatoid arthritis, asthma, diabetes etc. In the last few years, there has been a renewed interest in studying the antioxidant and anti-inflammatory action of plant constituents such as flavonoids and diarylheptanoids.AimTo evaluate the antioxidant, anti-inflammatory activity and the total phenolic content of isolated compounds from Alpinia officinarum rhizomes. Furthermore, molecular docking was performed to study the binding mode of these compounds into the active site of cyclooxygenase-2 (COX-2).MethodsA. officinarum rhizomes were extracted by maceration, using methanol. This extract was further fractionated by partitioning with hexane, chloroform and ethyl acetate and these fractions on further purification resulted in isolation of five pure compounds. Characterization was carried out by using ¹H NMR, ¹³C NMR and MS. They were further evaluated for antioxidant and anti-inflammatory activity using carrageenan-induced paw edema model in rats. Molecular docking study was performed using Glide module integrated in Schrodinger molecular modeling software.ResultsThe compounds were identified as 1,7-diphenylhept-4-en-3-one (1), 5-hydroxy-1,7-diphenyl-3-heptanone (2), 3,5,7-trihydroxyflavone (Galangin, 3), 3,5,7-trihydroxy-4′-methoxyflavone (Kaempferide, 4) and 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl)-1-phenyl-3-heptanone (5). The compound-3 and compound-5 (10 mg/kg) showed significant (p < 0.001) antioxidant and anti-inflammatory potential. Moreover, total phenolic content was detected as 72.96 mg and 51.18 mg gallic acid equivalent respectively. All the five isolates were found to be good binders with COX-2 (average docking score − 9.03).ConclusionsGalangin and 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl)-1-phenyl-3-heptanone exhibited anti-inflammatory and in-vitro antioxidant activity which may be due to presence of phenolic content in it. The molecular docking study revealed that these compounds have affinity towards COX-2 active site which can further be explored as selective COX-2 inhibitors. The results obtained in this work justify the use of A. officinarum in the treatment of inflammatory disorders like rheumatoid arthritis and inflammatory bowel diseases.     

针药并用治疗书写痉挛症5例

Combined acupuncture and Chinese herb decoction were applied for 5 cases with graphospasm. Acupuncture included scalp acupuncture and body acupuncture. Using transcranial repeated needling, Băihuì (百会GV20), Chorea-trembling controlled area, Emotional area, as well as Dàzhuī (大椎GV14), Fēngchí (风池 GB20), and Yíngxiāng (迎香EX-HN8) etc. were stimulated by proposed by Sun Shentian. Meanwhile, the patients took orally Chinese herb decoction, on the basis of the Sìwù decoction (四物汤four substances decoction) addition and subtraction, relieve symptoms under syndrome differentiation. The treatment was carried out for 4 courses in total. One course consisted of 5 days, with an interval of 2 days between two courses. After treatment of 5 patients, 2 patients were cured, 2 patients achieved markedly effective response, and 1 patient achieved effective response.