miR-99a对帕金森模型细胞活力及ROS水平的影响

目的:分析miR-99a对帕金森病细胞模型作用。方法:采用Western blotting以及Real-time PCR方法,对miR-99a以及其靶基因FZD5表达水平进行研究。采用CCK-8方法,检测细胞存活力。结果:过表达miR-99a,可将MPP+抑制PC12细胞存活作用明显缓解,该作用是通过调控miR-99a/FZD5轴完成的。结论:miR-99a在帕金森病细胞模型中,可对MPP+导致的PC12细胞存活抑制作用明显缓解,是通过靶向及下调FZD5实现的。     

精神科护理管理中的以人为本理念探讨

目的探讨"以人为本"的护理理念在精神科护理中的应用价值。方法选取自2016年6月-2018年5月期间该院收治的100例精神病患者作为实验研究对象,随机将100例患者分为实验组与对照组,对照组患者采用常规方法进行护理,护理人员在"以人为本"的理念的指导下对实验组患者进行护理。6个月后比较两组患者的疗效及IPROS评分。结果经过一段时间的护理,采用住院精神病患者康复疗效评定量表(IPROS)对两组患者治疗前后的评分进行比较分析。护理前,两组患者的评分对比差异无统计学意义(P>0.05)。护理后,两组患者的评分明显低于对照组患者的评分。实验组中,33例对护理满意,15例基本满意,不满意2例,护理满意度为96.00%;对照组中,20例对护理满意,17例基本满意,不满意13例,护理满意度为74.00%。护理满意度方面实验组明显高于对照组,两组差异有统计学意义(P<0.05)。结论在精神病患者的临床护理中,遵循"以人为本"的护理理念,能够在很大程度上改善精神病患者的临床护理效果,大大提升护理满意度,从而达到满意的治疗效果,因此,"以人为本"的护理理念值得在临床实践中进行推广。     

A 16-plex Y-SNP typing system based on allele-specific PCR for the genotyping of Chinese Y-chromosomal haplogroups

Y-chromosomal SNP (Y-SNP), with its stable inheritance and low mutation, can provide Supplementary information in forensic investigation. While commonly used Y-chromosomal STR haplotypes show their limitations, typing of Y-SNP would become a powerful complement. In this study, a 16-plex Y-SNP typing system based on allele-specific PCR (AS-PCR) was developed to discriminate four dominant Y-chromosomal haplogroups (C-M130, D-CTS3946, N-M231, and O-M175) and 12 predominant sub-haplogroups of O-M175 (O1a-M119, O1a1a1a-CTS3265, O1b-M268, O1b1a2-Page59, O2-M122, O2a1-L127.1, O2a1b-F240, O2a1b1a1-CTS5820, O2a2-P201, O2a2b1a1-M177, O2a2b1a1a1a-Y17728, O2a2b1a2-F114). A series of experimental validation studies including sensitivity, species specificity, male-female mixture and inhibition were performed. The discrimination of the typing system was preliminarily proved with a haplogroup diversity of 0.9239. Altogether, the Y-SNP typing system based on AS-PCR should be capable of distinguishing China’s dominant Y-chromosomal haplogroups in a rapid and reliable manner, thus can be employed as a useful complement in forensic casework.     

慢性氟中毒对大鼠血清氧化应激反应的影响

目的探讨不同剂量氟化钠对大鼠血清抗氧化系统的影响。方法给4组Wistar大鼠分别饮用含0、125、250、500mg/L的氟化钠去离子水溶液6个月,复制慢性氟中毒大鼠模型。6个月后眶静脉取血,测定血清抗氧化酶谷胱甘肽过氧化物酶（GPX）、超氧化物歧化酶（SOD）活力,丙二醛（MDA）和一氧化氮（NO）的含量。结果与对照组相比,染氟组大鼠血清GPX、SOD活力显著下降,MDA和NO含量显著增加。结论高剂量氟可以破坏大鼠的抗氧化系统,使其处于氧化应激状态。     

特色蒙药资源保护与开发利用现状及发展战略简析

蒙药资源是蒙医事业发展的源头和保证,特色蒙药资源是蒙药资源中更具地域性、优质、具有鲜明民族用药特色的蒙药资源,在蒙医药领域有着不可替代的重要地位。然而近年来,由于生态环境破坏、需求量激增等原因,许多特色蒙药资源处于濒危状态,对其保护与开发利用仍存在诸多问题,必须予以足够的重视。该文从特色蒙药资源种类、保护与开发利用现状等方面进行探讨,并在此基础上对特色蒙药资源保护与开发利用今后的发展战略进行了简析,旨在为蒙医药事业快速健康发展提供一点借鉴。     

微信与常规干预模式对幼儿园家长手足口病健康教育干预效果研究

目的对幼儿园儿童的家长采用微信与常规模式的不同健康教育干预模式,比较分析6个月后的干预效果,为疾病预防控制部门建立良性沟通的信息反馈平台,以及制定符合新媒体时代的手足口病防治策略和措施提供科学依据。方法通过健康教育的方式,对九原区4所幼儿园家长分别进行常规干预和微信干预,比较分析干预前、干预后6个月手足口病防控知识、日常行为及态度等因素,判断其干预效果,并通过Logistic多因素回归分析筛选影响家长日常行为的因素。结果健康教育后6个月家长对于手足口病的知识得分均有提高,微信组效果较好;但家长日常行为没有明显改善;Logistic多因素回归分析,对于家长日常行为的影响因素主要是家庭居住地。结论微信干预6个月后知晓率效果优于常规干预方式,家长日常行为未见明显效果,提示在干预方式上需加强日常行为干预。     

Genetic polymorphism of both 29 Y-STRs and 213 Y-SNPs in Han populations from Shandong Province,China

Genetic markers on the Y chromosome, including short tandem repeats (Y-STRs) and single nucleotide polymorphisms (Y-SNPs), are used widely in forensic genetics. Both Y-STR-based haplotypes and Y-SNP-based haplogroups provide information on a population’s genetic structure, which is useful for the identification of individuals. However, there are few studies on these two types of genetic markers in the various Chinese populations. In this study, 284 Han individuals from four prefecture-level cities in Shandong Province (Binzhou, Dezhou, Heze, and Weihai) were genotyped by 29 Y-STRs (from our previous study) and 213 Y-SNPs (self-designed for the Haplogroup O2 Y-SNP panel). Haplogroup O was the most predominant among the four cities. The highest haplogroup diversity (0.9745) was observed in the Heze population, with a discrimination capacity (DC) value of 0.5625. The haplotype diversity and DC values of the Binzhou and Heze populations were 1.0000. Furthermore, genetic differences were observed between the coastal and inland cities; the results of their statistical analysis are presented herein.     

Forensic and phylogenetic characterization of 15 autosomal STRs in Hazara population of Pakistan

In the current study, 217 unrelated individuals of the Hazara population were genotyped for 15 autosomal short tandem repeats to generate parentage and forensic efficacy parameters. Hazaras belong to the Shi’a sect and are recognized by their Turko-Mogholi features. We found that D2S1338 was the most discriminatory locus with a maximum power of exclusion and high value of polymorphism information content. Whilst the Combined Power of Discrimination (CPD), Combined Matching Probability (CMP) and Combined Power of Exclusion (CPE) were 0.999999999999999, 2.76796338879E^-17 and 0.999999040733479 respectively. Furthermore, the pattern of genetic affinity with genetically assumed related populations was demonstrated through Heat Map and Phylogenetic analysis, which revealed a great level of genetic closeness of Hazaras with Mongol population and descendants of Genghis Khan. The resulting data can be used for forensic applications and anthropological studies.     

Could a “body fragmentation index” be useful in reconstructing events prior to burial: Case studies of selected primary and secondary mass graves from eastern Bosnia

This study analyses variable levels of body fragmentation among secondary mass grave sites with similar formation process history. The study is based on data from 10 commingled secondary mass grave sites and two primary sites related to the war in Bosnia in 1995. The aim was to investigate differences in level of body fragmentation between mass graves of similar origin and taphonomy. In order to quantify the degree of fragmentation (and level of commingling) within a grave, we introduced a fragmentation index (FI). FI represents the ratio between the number of complete bodies and number of body parts from the same context. Results show high discrepancies in body fragmentations between different sites. FI for secondary sites of similar formation history varied from 0.01 to 0.59 (max = 1), while two primary sites have values 0.92 and 0.90 respectively. Variable levels of fragmentation among similar secondary sites suggest a possibility of different peri mortem circumstances of buried, so we tested whether the “body fragmentation index” could assist in elucidating the manner of death. Unusually high levels of body fragmentation (FI value below 0.1) in some secondary sites may indicate that body disarticulation was most likely caused peri-mortem by explosives, land mines, mortars or tank fire, all suggesting a combat situation.