电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

方剑乔教授运用针灸“四步疗法”治疗踝关节扭伤临床经验

<正>吾师方剑乔教授系国家级名中医、博士生导师,浙江省针灸学会会长,从事针灸教学、科研和临床工作30余年。方师早年留学日本,博采众长,学验俱丰,擅长中医经典理论与现代医学相结合,将其应用于疼痛及免疫相关疾病,尤其对痛证的诊治颇具造诣。踝关节扭伤是一种常见的软组织损伤性疾病,在关节韧带损伤中占首位[1],大部分踝关节扭伤可以进行非手术治疗[2-3],但如果治疗不当,则会有20%~40%     

穴位天灸结合凉血活血药治疗肝炎后高胆红素血症

自 1 998年 4月以来 ,笔者采用穴位天灸结合凉血活血中药治疗慢性肝炎高胆红素血症 ,疗效满意 ,现报告如下。1　资料与方法1 .1　一般资料 :慢性肝炎后高胆红素血症患者 71例 ,均为慢性乙型肝炎恢复期患者 ,慢性乙型肝炎按1 995年全国传染病会议拟定的标准诊断 ,入选患者肝功能检查血清总胆红素 ( TBil) >1 7.1 μmol/L,并排除肝外梗阻性黄疸 ,丙氨酸转氨酶 ( ALT)、天门冬氨酸转氨酶 ( AST)正常或轻度异常 ,其他临床症状轻微者。其中男 49例 ,女 2 2例 ,肝炎病史 1 .2～ 8.3年。 TBil2 5 .1～ 63.7μmol/L。上述患者按治疗先后随机分…     

艾灸百会穴对AD大鼠学习记忆能力及海马区CaMKⅡ mRNA表达的影响

目的:观察艾灸百会穴对阿尔茨海默氏病(AD)大鼠学习记忆能力和海马区钙调蛋白激酶Ⅱ(CaMKⅡ)mRNA表达的影响。方法:用Morris水迷宫进行筛选建立AD大鼠模型。将符合模型标准的20只AD大鼠随机分模型组、艾灸组,每组10只。另外随机选取10只正常老年大鼠为对照组。艾灸组用特制艾条在百会穴施温和灸,共治疗6个疗程。治疗结束后进行Morris水迷宫测试大鼠学习记忆能力,并用RT-PCR法检测大鼠左侧海马组织CaMKⅡ表达水平。结果:模型组平均逃避潜伏期较对照组和艾灸组明显延长(P<0.05,P<0.01),有效区停留时间较对照组和艾灸组明显缩短(P<0.05,P<0.01);上述二指标艾灸组与对照组比较均无显著性差异(P>0.05)。三组之间平台停留次数无显著性差异(P>0.05)。模型组左侧海马区CaMKⅡmRNA相对表达量较对照组和艾灸组显著下降(P<0.05,P<0.01),艾灸组与对照组比较无显著性差异(P>0.05)。结论:艾灸百会穴可通过上调海马区CaMKⅡmRNA表达以提高AD大鼠学习记忆能力。     

银杏叶提取物对慢性阻塞性肺疾病急性加重期患者血管内皮功能的影响

目的探讨银杏叶提取物对慢性阻塞性肺疾病急性加重期（AECOPD）患者血管内皮功能的影响。方法将60例AECOPD患者随机分为常规治疗组（CT组）,银杏叶组（GBLE组）,每组30例;并选择同期30例健康体检者作为对照组。两组常规治疗相同,GBLE组每日加用舒血宁注射液20 mL静脉滴注,连用2周。酶联免疫吸附法检测血清一氧化氮（NO）、血栓调节蛋白（TM）和血管假血友病因子（vWF）。结果两个治疗组治疗前NO,TM显著低于对照组（P〈0.01）,vWF显著高于对照组（P〈0.01）。治疗后,两组NO,TM水平较治疗前显著升高（P〈0.01）,而GBLE组升高更为显著,与CT组比较有统计学差异（P〈0.01）;两组vWF水平较治疗前显著下降（P〈0.01）,GBLE组下降较CT组更为显著（P〈0.01）。结论银杏叶提取物有显著改善AECOPD患者血管内皮细胞功能的作用,可预防血栓性疾病。     

电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

Objective To observe the effect of nerve growth factor(NGF)and electro-acupuncture(EA) on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF(10μg/kg,once daily for 15 days)via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui(DU20)and Yamen(DU15)acupoints(frequency 100 Hz,2 mA,20 min/time,once daily for 15 days).Morris' water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats' learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF+EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.     

石菖蒲煎剂对不同时长急性脑缺血再灌注大鼠BBB保护效应的研究

目的:证实石菖蒲煎剂对血脑屏障有保护作用,研究石菖蒲煎剂对不同时长急性脑缺血再灌注大鼠血脑屏障(Blood Brain Barrier BBB)保护效应,并探索石菖蒲煎剂治疗延长脑缺血溶栓时间窗的可能性。方法:将96只成年雄性SD大鼠随机分为4组,即假手术组、模型组、单纯再灌组、石菖蒲再灌组,每组24只;各组随机分为MCAO3h,MCAO6h 2个亚组,每组12只;复制大鼠大脑中动脉缺血模型MCAO,假手术组行假手术。在溶栓后24 h,每个亚组随机分成两组,每组6只,分别用荧光法测定大鼠脑内伊文斯蓝(EB)的含量以及大鼠脑含水量和脑系数。结果:1MCAO3h模型,与单纯再灌组比较,石菖蒲再灌组脑含水量、脑系数、脑EB含量无明显差异(P0.05);与假手术组比较,石菖蒲再灌组与脑含水量无明显差异(P0.05),脑系数提高(P0.05);2MCAO6h模型,与单纯再灌组比较,石菖蒲灌再灌组脑含水量、脑系数及脑EB含量均下降(P0.05);与模型组比较,石菖蒲再灌组脑含水量及脑系数明显下降(P0.05),单纯再灌组脑EB含量升高(P0.05);3与3 h石菖蒲再灌组比较,6 h石菖蒲再灌组脑EB含量升高(P0.05)。结论:石菖蒲煎剂对血脑屏障有保护作用,能改善其通透性,降低缺血再灌注损伤;在一定时间内,脑缺血时间越长石菖蒲煎剂对血脑屏障的保护作用越明显;石菖蒲水煎剂具有延长大鼠溶栓治疗时间窗的可能性。     

Effects of Hegu （LI 4） and Taichong （LR 3） on Vascular Dilation and Constriction in Migraine Patients

Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmonize yin and yang, regulate qi and blood, and balance ascending or descending. This paper attempts to study the effects of Si Guan point on the vascular dilation and constriction in migraine patients from the perspective of its feature.     

谈中医专业《针灸学》的教与学

针灸学是中医学的一个重要分支学科,<针灸学>是高等院校中医专业本科的主干课程,对该课程的教与学的好坏,在一定程度上影响中医专业学生的培养质量.根据我们往年的课堂情况及教学反馈信息,由于针灸学内容的特殊性及部分学生对专业的认识、导向等问题,对<针灸学>的学习仍处于一种茫然、被动状态,部分同学甚至存有学完针灸后将来有何用途的疑虑.为此,我们从2001年开始,对<针灸学>的教学作了一些尝试性的改革,并取得了较好的教学效果.     

从“筋”论治颈源性头痛探析

颈源性头痛的发病机制在于＂筋损致病＂,即颈部经筋急性损伤或慢性劳损后所产生的＂炎症＂刺激引起头痛.治疗上应根据＂顺筋取穴＂＂筋穴治痛＂的原则,以经筋起止点、压痛点、压痛缓解点、筋结点为主穴,施以震颤松解手法和温针灸法,以达筋松脉通、通则不痛之效.