精子线粒体DNA损伤与男性不育

精子线粒体DNA与男性生育能力密切相关,由于它缺少组蛋白和DNA结合蛋白的保护,易受活性氧的攻击,造成损伤,增加突变率,这被认为是引起男性不育的重要原因。精子mtDNA突变和数量的检测是评价男性生育力的重要方法。对精子mtDNA损伤的研究有重要意义。     

聚精丸对弱精子症患者精子DNA完整性的影响

目的探讨聚精丸对弱精子症患者精子DNA完整性的影响。方法根据WHO标准选择弱精子症患者59例,常规服用聚精丸3个月。应用吖啶橙试验（AOT）和计算机辅助精液分析（CASA）法,分别检测并对照分析治疗前后精子DNA完整性指标（DFI）和精液参数变化。并将59例患者按照精子DNA损伤参数（DFI）值异常与否分层研究。结果治疗3个月后,患者精子密度、总数、活力、活率、畸形率、DFI与治疗前相比差异有统计学意义（P〈O．01）;正常组DFI与治疗前相比差异无统计学意义（P--0．581）,异常组DFI与治疗前相比差异有统计学意义（P=0．000）。结论聚精丸可显著提高弱精子症患者精子密度、总数、活力、活率和精子DNA完整性,聚精丸是治疗弱精子症和精子DNA损伤的有效药物。     

少精子症辨治体会

少精子症的临床辨治当把握辨证首分虚实两端、辨证需与辨病相结合及重视抓脾胃主症等原则。治疗以健脾补肾活血为指导,提出行之有效的方剂:三子生精汤、清源固本汤及化结通精汤。临床治疗当熟悉中药现代药理研究,进行中药靶向治疗及采用中西医结合方案,相得益彰,从而发挥中药之长处。     

衣原体感染与男性不育

衣原体感染与男性不育南京医科大学组胚教研室（南京２１００２９）周亚东葛百明吴瑞娟祝辉江苏省中医院男性科翟亚春何映徐福松男性生殖道沙眼衣原体（Ｃｈｌａｍｙｄｉａｔｒａｃｈｏ－ｍａｔｉｓ，ＣＴ）感染对男性生殖功能的影响已引起人们的重视；ＣＴ是生殖道上皮...     

以"风胜湿"理论论治慢性前列腺炎

慢性前列腺炎的主要症状为排尿异常和局部疼痛。近年来主要从免疫反应、盆底神经肌肉紧张、尿液返流、炎症、长期充血、前列腺结石等方面阐述其发病机制．并认识到，本病往往是多种因素通过不同的机制共同作用的结果．其中可能有一种或几种起关键作用。从中医辨证角度分析．病机是肾虚湿热、清浊相混、精离其位。病理因素主要是湿、热、瘀、虚四端。其中最为重要的是湿和热，再细究其主要矛盾和中心环节是湿邪为患。笔者归纳为：（1）审部求因。多湿；（2）湿性重浊粘滞，久伏存留；（3）湿滞，精浊、精瘀之变；（4）湿兼它邪为患，多见湿热；（5）湿伤阳，易伤脾；（6）湿之根由在本虚。     

从肝辨治阴茎勃起功能障碍1例

阴茎勃起功能障碍（ED）中医治疗自古以来就是异彩纷呈，流派众多，其途有异，其归则一。其中主要的是从肾论治，由肾主生殖、肾藏精、肾司二阴、肾为作强之官所决定。现代社会的男性多因饮食结构变化和竞争激烈的社会环境，致使精神压力越来越大，精神因素影响成为功能性ED的病因，既是整个大环境的改变，也是导致ED的病机向实证转化的成因。ED从肝论治的适应症：中青年、部分老年，亚健康人群（疲劳综合征、紧张性高血压病）；主要为功能性ED；性欲改变。常见中医证型为邪客肝脉：七情、湿、热、痰、瘀等，以邪实为主。肝经自病：肝气郁结、肝经湿热、肝血瘀阻，多有郁滞；他脏相病：肾虚、心脾两虚，多为失养失调；主要见于实证或虚实夹杂证。     

Treatment of Patients with Erectile Dysfunction by Shugan Yiyang Capsule(疏肝益阳胶囊):A Multi-Centered Randomized Controlled Trial

Objective: To evaluate the efficacy and safety of Shugan Yiyang Capsule (SGYY, 疏肝益阳ficiency Syndrome type with or without blood stasis. Methods: A multi-centered, randomized, double-blinded, placebo-controlled trial in 304 patients was conducted by dividing them into 3 groups, who received re101), 1.0g taken three times a day for 4 weeks. Besides, 205 patients were arranged in an open-label studyand treated with SGYY 1.0 g taken three times a day for 4 weeks. The total effective rate, total remarkablyeffective rate, erection recovery rate tested by penis hardness test ring, time taken for improving erection among groups, as well as therapeutic effect between psychogenic ED group and mild arteriogenic ED group were compared. Adverse reactions related to SGYY were recorded. And a 3-month follow-up study was conducted on 105 cured patients. Results: The trial was completed in 500 patients. The total effective rate and total remarkably effective rate in the SGYY treated double-blinded group were 88.0% and 64.0% respectively, and those in the open-label group 90.5% and 65.0%, respectively, all significantly higher th an thosein the placebo (21.0%, 6.0%) and SYBS groups (60.0%, 29.0%), P＜0.01. At the same time, the e rection recovery rate and improving time in SGYY group were significantly better than those in other groups ( P＜0.01). No adverse reactions related to SGYY were found. The successful rate of potency to have intercourse within 3 months was over 80% (100 patients out of the 105 cured patients). Conclusion: SGYY was an effective and safe treatment for patients with psychogenic and mild arteriogenic ED of Gan stagnation and Shen deficiency Syndrome type with or without blood stasis, especially for psychogenic ED patients.     

齐墩果酸抗环磷酰胺所致大鼠肝细胞损伤作用

目的研究齐墩果酸（OA）对环磷酰胺所致大鼠肝细胞损伤的保护作用。方法分离大鼠肝细胞，进行原代培养。检测实验大鼠肝细胞上清液中丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）和肝细胞MTT值。结果环磷酰胺可使肝细胞上清液ALT、AST及LDH活力升高，肝细胞MTT值减小，齐墩果酸能抑制上述变化。结论齐墩果酸可抗环磷酰胺所致肝细胞损伤。