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1.
Suppurative corneal ulceration in Bangladesh 总被引:8,自引:0,他引:8
AAS Dunlop MB BS ED Wright MRCPath † SA Howlader‡ I Nazrul‡ R Husain‡ K McClellan FRACO § FA Billson FRACO § 《Clinical & experimental ophthalmology》1994,22(2):105-110
Suppurative keratitis is an important preventable cause of blindness, particularly in the developing world. This study analyses 142 cases of suppurative keratitis referred to Chittagong Eye Infirmary, Bangladesh. Some 53.5% of cases were bacterial and 35.9% were fungal. The five most common pathogens were: Pseudomonas sp. 24%, Streptococcus pneumoniae 17%, Aspergillus sp. 13%, Fusarium sp. 7% and Curvularia sp. 6%. Gram stain and culture results were consistent in 62.6% of cases. Previous antibiotic treatment was a significant factor for failure of culture isolation and less so for Gram stain failure. On Gram stain, 55.9% of pseudomonal cases were missed, but only 2% of fungal cases were missed. Over all, Gram stain had a sensitivity of 62% and positive predictive value of 84% for bacterial cases, and 98% and 94% for fungal cases, respectively. Fungal ulcers were typically filamentous, but an antecedent history of trauma was not common. The most frequent injury was due to rice grains, but the inoculum appeared to be introduced during eye washing with contaminated water. Pseudomonal ulcers occurred most frequently in the monsoon season, and Fusarium cases were seen only in the hot, dry season. 相似文献
2.
河北磁县食管癌胃癌高发人群口腔真菌分布的调查 总被引:1,自引:0,他引:1
应用常规方法随机研究食管癌、胃癌高发的磁县人群547例的口腔真菌。结果:1.口腔真菌阳性率为68.4%,食管真菌阳性率为47.3%,前者显著高于后者(P<0.001)。2.口腔真菌以念珠菌、曲霉菌和青霉菌为主,食管真菌以曲霉菌、青霉菌和念珠菌占多数。3.口腔与食管内的产毒真菌差异无显著性意义(P>0.05)。本结果为进一步研究口腔真菌与上消化道癌及肿瘤的一级预防提供了依据。 相似文献
3.
Frances M. Dewey David R. Twiddy Sarah I. Phillips Margaret J. Grose Peter W. Wareing 《Food and Agricultural Immunology》1992,4(3):153-167
A sensitive, specific, quantitative enzyme‐linked immunosorbent assay (ELISA) has been developed that can be used to determine the extent of mycelial growth of a sporulating thermophilic fungus, Humicola lanuginosa on the surface of rice grains. The assay employs a monoclonal antibody EC6, developed in a previous study, which does not recognize spores of the fungus. Using antigen‐coated wells, a direct linear relationship was established between dilutions of extracts from freeze‐dried mycelium (0.5 to 3 μg/ml) and absorbance values but to eliminate day‐to‐day variations it was found to be necessary to run a dilution series, prepared from stock freeze‐dried mycelium, with every test sample. The ELISA method was compared with conventional quantitative methods. Estimates of total mycelial length in freeze‐dried material by ELISA were found to be in the same order of magnitude as those determined by ergosterol and a theoretical calculation. The ELISA method also compared favourably with direct linear measurements (by photomicrography) of live mycelium present in aliquots from homogenates of a 1 cm2 plug taken from a plate but estimates of the latter by the dilution plate count method were much lower. In assays with inoculated rice grains, the quantitative ELISA method proved more sensitive than either the ergosterol method or direct plating of surface‐sterilized grains. The ELISA method also has the advantage of being highly specific and quick to conduct. 相似文献
4.
The ribosomal DNA from the Zygomycete Mucor miehei has been characterised. The complete rDNA unit was cloned by heterologous PCR using primers whose sequence matched conserved
regions of the rDNA from related fungal species. The sequence of the overlapping PCR products revealed the existence of a
repeated unit of 9574 bp. The genes encoding the different rRNA species were identified by their homology to the corresponding
sequences from other fungi. We estimate that the rDNA unit is present in the genome of M. miehei in about 100 copies. This estimation was made by comparing the intensity of its hybridisation signal in a Southern blot with
that of the mmp gene coding for aspartyl protease, which was assumed to be contained in single copy. The size and structure of the M. miehei rDNA unit was similar to that of other fungi. The genes encoding the 25S, 18S and 5.8S RNAs are closely linked within the
repeated unit which also contains the 5S gene. This latter gene appears to be transcribed in the opposite direction. The 25S,
18S and 5.8S genes showed 70–80% homology to the corresponding genes from other fungi, whereas the degree of homology for
the 5S gene was much lower. The highest homology (about 80%) corresponded to the few available sequences from other Mucor species. Homology to genes from other Zygomycota was no higher than that observed for genes from the Ascomycota or Basidiomycota fungi.
Received: 21 December 1999 / 1 March 2000 相似文献
5.
Keywords: 《Human pathology》1991,22(12)
We report two cases of allergic bronchopulmonary fungal disease (ABPFD) caused by Curvularia sp and associated with allergic fungal sinusitis (AFS). Curvularia lunata was cultured in one case and Curvularia senegalensis was cultured in the other. Based on these cases and a review of the literature, we discuss unusual clinical and pathologic features that can occur in ABPFD. Unusual clinical aspects of ABPFD include associated AFS, absence of asthma, progression to Churg-Strauss angiitis and granulomatosis, concomitant hypersensitivity pneumonitis, and underlying cystic fibrosis. Atypical pathologic features that may occur in ABPFD include follicular bronchiolitis, xanthomatous bronchiolitis, limited tissue invasion, fungus balls, and association with unusual fungi. Prominent follicular bronchiolitis and xanthomatous bronchiolitis were misleading histologic features in one of our cases and led to a delay in recognition of the diagnosis. Both patients presented primarily with AFS; ABPFD was detected subsequently. This suggests that a small subset of patients with AFS may be at risk for ABPFD. The goal of this review is to increase awareness of unusual clinical and pathologic manifestations of ABPFD. It is hoped that this will result in accurate diagnosis and proper therapy, especially for patients who present with atypical features. Unusual fungal species should be considered in patients who have clinical findings compatible with ABPFD but who do not demonstrate immunologic reactivity to Aspergillus sp, especially Aspergillus fumigatus. In addition, ABPFD should be considered in patients with AFS who develop new pulmonary lesions. 相似文献
6.
A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1- (TEF1) adjacent to the 5 end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (TEF1p/HPT) transformed A. pullulans strain R106 to a hygromycin B-resistant (HmBR) phenotype. A PCR-generated DNA fragment consisting of the TEF1p/HPT resistance marker flanked by 41 bp of homologous DNA has also been shown to transform A. pullulans to HmBR. Linearized plasmid DNA consistently produced more transformants than circular plasmid DNA. Analyses of 23 HmBR transformants revealed integration of the plasmid in only eight of these transformants. In two transformants, integration into the largest chromosome (VIII) resulted in an alteration of the molecular karyotype. In four other transformants, integration occurred in chromosome VI (the chromosome containing TEF1) but only one was the result of homologous recombination with the genomic copy of the TEF1 promoter. The remainder of the transformants contained replicative plasmids that could be visualized on an agarose gel by ethidium bromide staining. These plasmids were generally 7–8 kb in size. One transformant appeared to contain four plasmids ranging in size from 4 to 8 kb, suggesting rearrangement of the transforming DNA. One plasmid obtained from a HmBR
A. pullulans transformant was able to transform E. coli to ampicillin resistance. However, after recovery from E. coli, this plasmid (approximately 4 kb) was unable to transform A. pullulans to HmBR. 相似文献
7.
百特药液治疗外耳道瘙痒症的临床研究 总被引:2,自引:0,他引:2
目的:筛选治疗外耳道瘫痒症的理想药物,以期从根本上治疗该病。方法:采用分组用药,并按双盲法观察疗效。试用药物为百特药液、1.0%酚甘油、氯霉素眼药水,4%硼酸酒精和生理盐水(对照)。结果:四种药物中百特药液对外耳道瘙痒症的治疗效果显著,近期治愈率达84%。结论:百特药液是临床上治疗外耳道瘙痒症大有希望的药物,值得在临床上推广试用。 相似文献
8.
A literature search was conducted to report all cases of documented transmission of infectious diseases from donors to recipients of corneal transplants. Fourteen such cases have been reported. There is no experimental or clinical evidence to suggest the transmissions of either hepatitis or syphilis via corneal grafting. Available evidence regarding a number of neurologic and other disorders in which a slow virus etiology has been implicated were reviewed. On the basis of this review, we are able to draw certain conclusions and guidelines for selection or rejection of donor material for transplant surgery. 相似文献
9.
慢性阻塞性肺疾病继发院内真菌感染的危险因素及药物经济学分析 总被引:2,自引:0,他引:2
目的 :分析慢性阻塞性肺疾病 (COPD)急性加重期继发院内深部真菌感染的危险因素及其药物治疗的经济学意义。方法 :查阅 2 0 0 0年 10月~ 2 0 0 1年 10月我院 74例COPD急性加重期患者的病历 ,对其抗生素应用种类、应用时间、糖皮质激素应用情况、真菌感染部位、菌种类型、抗真菌药物、住院费用及平均住院日进行分析。结果 :高龄及营养状况差是院内真菌感染的危险因素之一 ,而广谱抗生素长期大量应用则是造成真菌感染的重要因素 ,另外 ,合并应用糖皮质激素也是院内真菌感染的又一发病因素。继发院内真菌感染 ,明显延长了住院时间 ,增加了住院费用。结论 :减少院内真菌感染 ,对遏制医疗费用的增长 ,节约国家的卫生资源 ,具有重大意义 相似文献
10.