怀槐愈伤组织形成与异黄酮积累

目的 :考察怀槐愈伤组织形成与总异黄酮积累的关系。方法 :不同培养基诱导怀槐子叶和下胚轴产生愈伤组织 ,紫外分光光度术测定愈伤组织中总异黄酮并含量。结果 :NAA /BA及含 2 ,4-D组合有利子叶愈伤组织的诱导 ;MS ,N6 ,B5培养基适宜子叶形成愈伤组织 ,而SH较适宜下胚轴产生愈伤组织。愈伤组织具有异黄酮合成能力 ,含有NAA激素组合诱导的子叶愈伤组织总异黄酮含量较高 ,而 2 ,4-D/BA组合诱导的下胚轴愈伤组织总异黄酮含量较高 ,高浓度 2 ,4-D/KT组合抑制子叶和下胚轴愈伤组织积累异黄酮。愈伤组织根据发育的形态特征可分为 4种类型 ,以黄色、疏松、颗粒状的Ⅱ型愈伤组织中总异黄酮含量最高。结论 :培养基与激素组合影响愈伤组织的形成及发育形态特征 ,并反映在愈伤组织异黄酮合成差异上 ,获得的黄色、疏松颗粒状愈伤组织为后续的细胞液体悬浮培养及异黄酮合成代谢的调控研究奠定了基础。     

The effect of soy isoflavones on the development of intestinal neoplasia in Apc mouse

Data from epidemiological studies suggest that isoflavones in soy may have a protective effect on the development of colon cancer in humans. Therefore, we have investigated whether soy isoflavones will inhibit intestinal tumour development in Apc^Min mice. The mice were fed a Western-type high risk diet (high fat, low fibre and calcium) containing two different isolates of soy protein as a protein source. For the control and test groups this resulted in the administration of about 16 and 475 mg of total isoflavones per kg diet, respectively. As a positive control, a third group of mice was administered a low isoflavone diet supplemented with 300 ppm sulindac. No significant differences in the incidence, multiplicity, size and distribution of intestinal tumours were observed between Min mice fed low and high isoflavone-containing diets. However, a clear reduction in the number of small intestinal tumours was observed for the sulindac diet. Thus, in contrast to epidemiological studies, our results demonstrate that high amounts of soy isoflavones present in a Western-type high risk diet do not protect against intestinal tumour development in a relevant animal model such as the Min mice.     

胶束毛细管电泳测定豆粕及发酵豆粕中大豆异黄酮含量

 目的 建立测定豆粕及发酵豆粕中大豆异黄酮含量的毛细管电泳方法。方法 采用胶束毛细管电泳法(MECC),未涂层弹性石英毛细管(50 μm×60 cm,50 cm);压力进样,压力：3.0×103 Pa;进样时间：5 s;分离电压：17 kV;柱温：20 ℃;检测波长：254 nm;运行缓冲液：15 mmol·L-1 十二烷基硫酸钠、5%甲醇的10 mmol·L-1硼砂溶液(pH 9.4)。结果 测得6种大豆异黄酮组分在24 min内能完全分离并具有良好的线性关系;平均加样回收率（n=5）在 99.6%~103.5%之间,RSD为1.8%~2.3%。结论 方法准确、可靠、经济、操作简便,适合于豆粕及发酵豆粕中大豆异黄酮含量的测定。     

大豆异黄酮研究概况

介绍近年来大豆异黄酮研究概况,包括大豆异黄酮的提取分离、含量测定、生理活性及其在体内的吸收代谢。     

异黄酮衍生物的合成Ⅱ.7-氯和6-氯-7-甲氧基-3′-(N,N-二乙胺甲基)-4′-羟基异黄酮的合成

合成了7-氯和-6-氯-7-甲氧基-3′-(N,N-二乙胺甲基)-4′-羟基异黄酮(1478和1481)。它们的合成是由间-氯苯酚或3-羟基-4-氯苯酚与对-硝基苯乙酰氯反应,制得取代的脱氧安息香。它们与原甲酸乙酯环合得到取代的-4′-硝基异黄酮,再将化合物中的硝基用锌粉还原成氨基,再经重氮化和水解,得到取代的-4′-羟基异黄酮。它们经Mannich反应,最后制得7-氯和6-氯-7-甲氧基-3′-(N,N-二乙胺甲基)-4′-羟基异黄酮。它们耐氧作用不如已合成的7-甲氧基-3′-(N,N-二烷胺甲基)-4′-羟基异黄酮。     

Puerarin inhibits angiotensin II-induced cardiac hypertrophy via the redox-sensitive ERK1/2, p38 and NF-κB pathways

Aim： To investigate the effects of puerarin （Pue）, an isoflavone derived from Kudzu roots, on angiotensin II （Ang II）-induced hypertrophy of cardiomyocytes in vivo and in vitro.
Methods： C57BL/6J mice were infused with Ang II and treated with Pue （100 mg·kg-1·d-1, po） for 15 d. After the treatment, systolic blood pressure （SBP） and left ventricular wall thickness were assessed. The ratios of heart weight to body weight （HW/BW） and left ventricular weight to body weight （LVW/BW） were determined, and heart morphometry was assessed. Expression of fetal-type genes （ANP, BNP and β-MHC） in left ventricles was measured using semi-quantitative RT-PCR. Mouse primary cardiomyocytes were treated with Pue （50, 100, 200 μmol/L）, then exposed to Ang II （1 μmol/L）. ROS level was examined with flow cytometry, the binding activity of NF-κB was determined using EMSA. Western blot was used to measure the levels of ERK1/2, p38 and NF-κB pathway proteins. [3H]leucine incorporation was used to measure the rate of protein synthesis.
Results： Oral administration of Pue significantly suppressed Ang II-induced increases in the myocyte surface area, HW/BW, LVW/BW, SBP and left ventricular wall thickness. Furthermore, Pue significantly suppressed Ang II-induced increases in ANP, BNP and β-MHC expression in the left ventricles in vivo. Treatment of cardiomyocytes with Pue （50–500 μmol/L） did not affect the viability of cardiomyocytes in vitro. Pretreatment of cardiomyocytes with Pue dose-dependently inhibited Ang II-induced increases in ROS production, NF-κB binding activity, protein synthesis and cell breadth. Furthermore, pretreatment with Pue significantly suppressed Ang II-induced activation of ERK1/2, p38 and the NF-κB pathway proteins and the expression of ANP and β-MHC in cardiomyocytes. The positive drug valsartan exerted similar effects on Ang II-induced cardiac hypertrophy in vivo and in vitro.
Conclusion： Pue attenuates Ang II-induced cardiac hypertrophy by inhibiting activation of the redox-sensitive ERK1/2, p38 and the NF-κB pathways.     

7-羟基异黄酮阿魏酸酯衍生物的设计与合成

目的：设计与合成7-羟基异黄酮阿魏酸酯衍生物。方法：从间苯二酚和对位取代苯乙酸开始,经多步反应,合成出目标化合物。结果：合成了7个7-羟基异黄酮阿魏酸酯衍生物,其结构经核磁共振氢谱、红外光谱、质谱确证。结论：所得到的7-羟基异黄酮阿魏酸酯衍生物均未见文献报道,可供进一步药理研究。     

大豆提取物抗氧化作用研究

目的:观察大豆提取物对12月龄大鼠抗氧化系统作用的影响.方法:选用SD雄性健康12月龄大鼠,随机分成空白对照组和低、中、高剂量4组.空白对照组给予无菌水,实验组每日分别灌服32、63、190 mg/kg剂量的大豆提取物,连续35 d.第36 d经眼眶取血,测定血清过氧化脂质丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、肝组织中脂褐质含量.结果:与空白对照组比较,中、高剂量组大鼠血清MDA含量、肝脏中脂褐质含量显著降低,高剂量组血清SOD活力升高.结论:大豆提取物具有明显的抗氧化作用.     

大豆异黄酮对脑缺血大鼠保护作用的研究

目的:建立大脑中动脉栓塞(MCAO)模型,观察大豆异黄酮(ISO)对脑缺血大鼠的保护作用。方法:健康成年SD大鼠随机分成6组:ISO低、中、高剂量组、VE组、模型组、假手术组。4w后前5组做MCAO模型,分别于造模后1h和6h磁共振检测抗氧化指标,用透射电镜进行神经细胞形态学观察。结果:与模型组相比,各ISO组大鼠血清SOD和GSH-PX活性、6h各兴趣区rADC、rDCavg、rFA值较高;血清MDA含量较低,差别均有统计学意义。ISO剂量与血清SOD和GSH-PX活性、6h各兴趣区rADC、rDCavg、rFA均呈正相关;而与血清MDA含量呈负相关。形态学观察显示,ISO高剂量组神经元细胞结构良好。结论:ISO可通过提高机体抗氧化能力对缺血性大脑损伤起到一定的保护作用,ISO低、中剂量组与VE组作用相近。     

大豆异黄酮对慢性肾损害大鼠肾功能及脂代谢的影响

目的观察不同剂量大豆异黄酮（SIF）对慢性肾损害大鼠肾功能及脂质代谢的影响。方法采用腺嘌呤灌喂方法建立肾损害大鼠模型,按体质量大小编号随机分为3组,每组9只。分别为肘照组,予同等量花生油灌喂;SIF小剂量组,予SIF6mg/（kg·d）;大剂量组,予SIF12mg/（kg·d）。观察第0天、第21天、第42天血浆甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白、血浆尿素、血清肌酐水平以及尿蛋白排泄量。结果SIF治疗组血浆尿素、血清肌酐水平下降及尿蛋白排泄量减少,血浆甘油三酯、总胆固醇、低密度脂蛋白下降,高密度脂蛋白水平升高,上述各项指标与对照组相比,差异均有统计学意义（P〈0．05）,SIF不同剂量组之间比较差异无统计学意义（P〉0．05）。结论低剂量SIF就可起到改善肾损害大鼠的脂代谢紊乱,减少尿蛋白排泄和稳定肾功能的作用。