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1.
XTT比色法测定细胞生长曲线   总被引:4,自引:0,他引:4  
目的:研究XTT比色法用于细胞生长曲线检测的可行性.方法:采用XTT比色法检测卵巢癌细胞株3AO的细胞生长曲线,并与MTT法相比较.结果:XTT比色法检测获得的3AO细胞生长曲线与应用MTT法获得的细胞生长曲线大致相同,且比MTT法更加快速简便.结论:XTT比色法不仅可替代MTT法用于细胞生长曲线的检测,而且优于MTT法.  相似文献
2.
姜黄素对子宫颈癌HeLa细胞的抑制作用   总被引:1,自引:0,他引:1  
王菁鹏  林青 《现代医药卫生》2006,22(16):2435-2437
目的:探讨姜黄素对子宫颈癌HeLa细胞的抑制作用及其机制。方法:以细胞培养,镜下观察细胞形态学改变和计数法测定生长曲线;用^3H-脱氧胸苷掺入法测定对DNA合成的影响,以MTT比色法检测给药后HeLa细胞的增殖抑制情况。结果:姜黄素作用HeLa细胞后,癌细胞生长延缓并萎缩,胞质粗糙,有大量颗粒状物堆积,而且药物浓度越大,形态学改变越明显;生长曲线测定、^3H-脱氧胸苷掺入法及MTT比色法实验结果显示,姜黄素对HeLa细胞的增殖和生长有显著的抑制作用并呈明显的时间一剂量依赖关系,当姜黄素浓度为20μg/ml以上时,其对HeLa细胞的掺入抑制率高于5-Fu 20μg/ml对该细胞的掺入抑制率。结论:姜黄素对HeLa细胞具有直接杀伤作用,其机制可能通过干扰细胞代谢,改变细胞外膜的性质抑制肿瘤细胞增殖。  相似文献
3.
To assess the applicability of statistical moments in biopharmaceutical studies, an urinary excretion trial was conducted with three doses—400, 500, and 600 mg of amoxicillin trihidrate capsules—in 15 healthy volunteers, using a latin square design. The statistical analyses were a two-way MANO VA and a growth curve technique. It was inferred, in the dose range studied, that the amount excreted during the 12 hr following administration, was proportional to dose and that the dose had no effect on either the mean or the variance of the residence time. The calculated minimum significant difference in the amount of amoxicillin excreted was 15% with respect to the mean value.  相似文献
4.
用改良肉汤5,50,750ml连续继代培养鸡大肠杆苗074,0107和078三个血清型菌株。在最终使用的肉汤预热至37℃条件下,测定各菌株不同培养时间的菌落形成单位,以菌数的对数作纵坐标,培养时间作横坐标,绘制出各菌株的生长曲线。在本试验条件下,3个血清型菌株具有相似的生长模式,均可划分为3个时期,即速生期、高峰期和稳定期;各菌株每个生长期所持续的时间大致相同;在肉汤预热至37℃条件下,接入经两次继代培养的菌种,6h即达生长高峰。  相似文献
5.
根据孕16-26周胎儿双顶径、头围、腹围及股骨长等的超声测量值拟合Rossavik数学模型[P=c(t)^k s(t)建立胎儿个体生长曲线,结果显示,20例正常单胎的超声参数与Rossavik模型的拟合效果好,孕28-41周胎儿各项参数的模型预测值与实测值比较均无显著差异。  相似文献
6.
Hazard assessment often needs to compare inhibition values of different test species and different test durations. But the three different methods to calculate inhibition in growth inhibition tests (final biomass, growth rate, area under the growth curve) may lead to very different and sometimes contradicting numerical sensitivities of the test species. This paper will depict why there are these different results and what consequences this has for the evaluation of results. Comprehensive discussion of different aspects will show that using growth rate may eliminate most of the problems occurring for comparisons between test species, different test times and different laboratories. The use of growth rate and the adaptation of toxicity levels maximises reproducibility, comparability and biological sensitivity of biotests.  相似文献
7.
Research on the mental health effects of quitting smoking is limited. Smokers with mental illness appear to be at a higher risk of unsuccessful smoking cessation. Recent work suggests they are at elevated risk for post-cessation increases in anxiety, depression and suicidal ideation. The current study tested the effects of successful and unsuccessful smoking cessation on short-term psychopathology in 192 community participants. Smoking cessation outcomes were classified using expired carbon monoxide levels that were taken at quit week, 1 and 2 week follow-up and 1 month follow-up. We found no psychopathology increases in participants who successfully quit smoking. For individuals struggling to quit our results partially supported a recently proposed struggling quitters hypothesis. However, the vast majority of individuals posited to be vulnerable by the struggling quitters hypothesis did not experience clinically significant increases in psychopathology. These findings have implications for clinicians whose clients are interested in smoking cessation.  相似文献
8.
9.
This study aimed to identify factors influencing trajectories of change in two outcome domains, frequency of substance use and quality of life (QOL), among adolescents receiving a brief evidence-based intervention. Participants were 106 adolescents, aged 13 to 21 years, who met criteria for a substance use disorder. The adolescents received a five-session intervention and completed four assessments over 12 months. Based upon a theoretical and empirical review, five putative predictors were tested: gender, age, severity of conduct disorder, severity of depression, and peer substance involvement. Results of a parallel-process latent growth curve model indicated that higher peer substance involvement and conduct severity predicted higher frequency of use at baseline, whereas higher peer substance involvement and depression severity predicted poorer QOL at baseline. Counter to predictions, higher depression severity predicted greater improvements in QOL following substance use treatment. The implications of baseline risk factors on adolescents' response to intervention are discussed.  相似文献
10.
The potential longitudinal effects of prenatal cocaine exposure (PCE) on language functioning were estimated from early childhood through early adolescence in a large, well-retained urban sample of 451 full-term children (242 cocaine-exposed, 209 non-cocaine-exposed) participating in the Miami Prenatal Cocaine Study (MPCS). The sample was enrolled prospectively at birth, with documentation of prenatal drug exposure status through maternal interview, and toxicology assays of maternal and infant urine, and infant meconium. Age-appropriate versions of the Clinical Evaluation of Language Fundamentals (CELF) were used to measure total, expressive, and receptive language at ages 3, 5, and 12 years. Longitudinal latent growth curve (LLGC) modeling of the data revealed an association between PCE (measured dichotomously as yes/no) and lower functioning in expressive and total language scores, after considering other sources of variation including child's age at testing, sex, prenatal exposure to alcohol, marijuana, and tobacco, and additional medical and social-demographic covariates. Analyses of level of PCE showed a gradient, i.e. dose-dependent, relationship between PCE level and expressive, receptive, and total language scores in the models controlling for age, child's sex, and other prenatal drug exposures. With additional covariate control these findings were most stable for the total language score. The evidence supports an inference about an enduring stable cocaine-specific effect on children's language abilities, with no effect on language growth over time in the longitudinal trajectory of language development.  相似文献
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