排序方式: 共有22条查询结果,搜索用时 15 毫秒
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Jiang-Yun Liu Sheng-Yin Li Jian-Yong Feng Yan Sun Xiao-Fei Sun 《Journal of Asian natural products research》2013,15(4):325-331
Phytochemical investigation of ethanol extract from the flowers of Trollius chinensis Bunge resulted in the isolation of two new flavone C-glycosides (1–2), along with 10 known compounds (3–12). The structures of the new compounds were established as 6?-(3-hydroxy-3-methylglutaroyl)-2″-O-β-d-galactopyranosyl orientin (1) and 6?-(3-hydroxy-3-methylglutaroyl)-2″-O-β-d-galactopyranosyl vitexin (2) on the basis of various spectroscopic analysis (including different 1D and 2D NMR spectroscopies, high-resolution electrospray ionization mass spectrometry) and chemical evidences. Bioassay showed that eight flavonoids inhibited complement activation on the classic pathway in vitro, with their IC50 values ranging from 0.88 to 4.02 mM, which may contribute to the applications of the herb in treatment of acute respiratory distress syndrome, etc. 相似文献
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叶菜型甘薯蔓尖黄酮类化合物含量在不同品种、部位和采收期的变化 总被引:3,自引:0,他引:3
目的:研究叶菜型甘薯蔓尖黄酮类化合物含量在不同品种、部位和采收期的变化。方法:在全国叶菜型甘薯新品种区域试验重庆点,测定和分析莆薯53、广菜薯2号和福薯7-63个品种蔓尖的叶片、叶柄和茎3个部位在6个采收时期的黄酮类化合物含量及其变化。结果:莆薯53、广菜薯2号和福薯7-6蔓尖黄酮类化合物质量分数在采收期间的变化分别介于9.60~19.98,12.93~25.08,9.33~25.16mg.g-1,品种之间有显著差异;3个品种叶片的平均质量分数在采收期间的变化为3.66~11.09mg.g-1,显著高于茎(4.03~7.79mg.g-1),茎显著高于叶柄(2.20~5.26mg.g-1);采收前期蔓尖黄酮类化合物含量显著高于采收后期。结论:在叶菜型甘薯的品种选育、栽培和产业化等过程中应充分考虑其蔓尖黄酮类化合物含量在不同的品种、不同的部位和不同的采收时期的显著差异。 相似文献
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荧光光度法测定福建组培金线莲中总黄酮含量 总被引:2,自引:0,他引:2
目的建立测定福建组培金线莲中总黄酮含量的荧光光度法。方法根据黄酮类化合物与Al3+能形成稳定的荧光络合物,采用95%乙醇超声提取福建组培金线莲中的总黄酮,以槲皮素为对照品,用荧光光度法测定总黄酮含量。结果在选定的实验条件下,槲皮素浓度与荧光强度具有良好的线性关系,线性范围为0.002~0.400μg/mL,检出限为0.0004μg/mL,线性方程F=2002.6C+5.5296,相关系数r=0.9995,测得福建组培金线莲中总黄酮含量(按干燥品计算)为73.98μg/g,平均回收率为99.95%,相对标准偏差为0.92%(n=5)。结论本法操作简便、快速、准确,适用于福建组培金线莲中总黄酮的含量测定。 相似文献
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10种黄酮类化合物对糖尿病致病机制中重要通路的抑制作用 总被引:1,自引:0,他引:1
目的:观察10种黄酮类化合物对糖尿病致病机制中4条重要通路的多靶点抑制作用,发挥中药多靶点、综合治疗的优势。方法:从正常大鼠脑组织中提取半纯化的蛋白激酶C(PKC)、肾脏组织中提取醛糖还原酶(AR)粗酶,Fenton试剂体外激发正常大鼠脑组织脂质过氧化生成丙二醛(MDA)及体外合成高级糖化终产物(AGEs),将上述酶或合成物与10种黄酮类化合物(终浓度为1 mg.mL-1)作用,观察黄酮类化合物对上述酶或合成物的抑制作用。结果:黄芩苷和灯盏花素对AR,PKC,AGEs及MDA均有不同程度的抑制作用。结论:上述结果提示此两种黄酮类化合物可以通过抑制上述4条通路来预防糖尿病及其并发症的发生,为进一步的机制研究及临床应用提供良好的实验依据。 相似文献
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荧光光度法测定银杏叶总黄酮含量的研究 总被引:17,自引:3,他引:17
目的:根据黄酮类化合物能与Al3+形成稳定的荧光络合物,建立一种测定银杏叶总黄酮的新方法。方法:用60%乙醇加热回流提取银杏叶有效成分,以芦丁为标样,采用荧光分光光度法,选择激发波长λex=436nm,发射波长λem=483nm,测定银杏叶中总黄酮含量,并进行加标回收实验,验证方法的准确性。结果:本方法检出限为1.27×10-9mol·L-1,线性范围在1.64×10-9~3.63×10-5mol·L-1之间,线性回归方程:Y=1.9313X+0.2705,相关系数r=0.9989,平均回收率99.8%~104.2%,相对标准差(RSD)1.94%。结论:本法操作简便、快速、准确,具有良好的分析应用前景。 相似文献
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A comprehensive review of the literature from 1975 to 1995 was conducted. Reports were identified using the Cancerline and Medline databases. The chemical structure, estrogen agonist and antagonist activity, and in vitro studies were reviewed and compared with those of tamoxifes. Also, the antitumor activity in cell lines and animal models was highlighted. The mechanisms of action, metabolism, route of administration, dose scheduling, antitumor activity, and side effects of each agent were reviewed and compared with those for tamoxifen, where available. Several new antiestrogens are at various stages of development. They are divided into nonsteroidal and steroidal antiestrogens. Among the nonsteroidal antiestrogens are agents like toremifene and droloxifene. Both produce response rates, and both have toxicity profiles that are comparable to tamoxifen. In addition, toremifene can chemosensitize estrogen receptor-negative tumors, reverse multidrug resistance, and affect tamoxifen-resistant tumors. The short half-life of droloxifene results in minimal serum accumulation, and therefore it may be sequentially used with other systemic therapies to maximize cell kill. ICI 182,780, a steroidal antiestrogen, is well tolerated, active in tamoxifen-resistant tumors, and has a potential role in the preoperative management of advanced breast cancer. Other potential benefits of these and other agents are discussed. Pure antiestrogens have documented antibreast cancer activity that is in some instances more potent than tamoxifen. Some have activity in estrogen receptor-negative or tamoxifen-resistant tumors. Others may help to reverse chemotherapy resistance. In addition, the potential role of these antiestrogens in radioimaging and their effect on lipid metabolism is also being recognized. 相似文献
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鸡血藤干、鲜品黄酮部位的高效液相指纹图谱比较 总被引:1,自引:0,他引:1
目的:建立鸡血藤黄酮部位的HPLC指纹图谱,通过比较同一产地鸡血藤干、鲜品药材的指纹图谱,考察两者化学成分种类和含量上的差异。方法:采用高效液相色谱法,DiamonsilC18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.2%磷酸溶液为流动相梯度洗脱,流速1.0 m L·min-1,检测波长280 nm,检测时间80 min。结果:分别建立了干、鲜鸡血藤黄酮部位的HPLC指纹图谱共有模式,标定了11个共有指纹峰,并指认了4个共有峰,发现干、鲜品在成分和含量上均有差异。结论:该方法简便、准确,具有良好的重复性,可为鸡血藤化学成分深入研究及其加工利用提供科学依据。 相似文献
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荧光分光光度法测定福建野生金线莲中总黄酮含量 总被引:1,自引:0,他引:1
目的:根据黄酮类化合物与Al^3+形成稳定的荧光络合物原理,建立一种准确测定金线莲中总黄酮的方法。方法:采用超声提取法用95%乙醇提取福建野生金线莲中的总黄酮,以槲皮素为对照品,用荧光分光光度法测定总黄酮的含量,并进行回收率试验。结果:在选定的条件下,槲皮素浓度与荧光强度呈正相关,线性范围2~400ng·mL^-1,检出限为0.4ng·mL^-1,线性方程Y=2098.1X+4.1678,r=0.9998,平均回收率为98.4%,RSD=1.5%。结论:本法操作简便、快速、准确,适用于金线莲中总黄酮的含量测定。 相似文献
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