HPLC测定姜黄素混悬剂中姜黄素的含量

目的：建立高效液相色谱法测定姜黄素混悬剂中姜黄素的含量。方法：采用Diamonsil~（TM）C_（18）（200 mm×4.6 mm,5μm）,流动相：乙腈-甲醇-水（35：45：20）,检测波长：430 nm,柱温：25℃,流速：1.0 ml·min~（-1）,进样量20 ml。结果：姜黄素在0.05~5.00μg·ml~（-1）范围内线性关系良好（r=0.999 8）,平均回收率为98.6%,RSD为1.3%（n=9）。结论：本法简单准确、重复性好,可用于姜黄素混悬剂中姜黄素的含量测定。     

Adaptive Focused Acoustics™ for Nanosuspensions to Enable Pharmacology Assessment of Poorly Soluble Molecules in Lead Optimization

In Drug Discovery, pharmacology studies often require benign formulation compositions for safe administration in animal models. Here, we applied Adaptive Focused Acoustics? (AFA) to a molecular scaffold with challenging physicochemical properties for intraperitoneal administration. Nanosuspensions can be prepared at small scales and provide broad applicability. Our results show that nanosuspension formulations prepared by AFA have improved PK performance relative to a DMSO solution formulation that is prone to precipitation in-vivo.     

希丹液抗肿瘤作用的研究

目的：探讨希丹液对小鼠移植性肿瘤的抑制作用和对环磷酰胺抑瘤的增效作用.方法：小鼠腋下接种经腹腔传代培养的癌细胞（H22EC、P388）.结果：希丹液对肝癌（H22）、艾氏腹水癌（EC）及淋巴细胞白血病（P388）具有明显的抑制作用（P＜0.05或P＜0.01）,对环磷酰胺抑制艾氏腹水癌EC具有明显增强作用（P＜0.01）,并可明显延长荷载肝癌（H22）小鼠的存活时间（P＜0.01）.结论：希丹液对小鼠移植性肿瘤有抑制作用和对环磷酰胺抑瘤有增效作用.     

吸入用布地奈德混悬液的处方和制备工艺的优化

目的 优化吸入用布地奈德混悬液的处方与制备工艺.方法 以沉降体积比与再分散性的综合评分为指标,采用正交试验法对处方进行优化;采用高压均质技术制备布地奈德混悬液,以单因素试验法优化制备工艺.结果 优选的助悬剂为羧甲基纤维素钠,润湿剂为吐温80,絮凝剂为枸橼酸钠,其用量分别为0.10％、0.05％、0.05％;优化的工艺条件为低压、均质2～3次;按优化后的处方和工艺所制备的布地奈德混悬液的平均粒径为1.70±0.15 μm,平均Zeta电位为-24.1 +0.4 mV,布地奈德的含量为标示量的99.3％.结论 按优化后的处方和工艺所制备的混悬液的理化稳定性良好,达到了吸入用混悬液的质量要求.     

细胞悬液移植技术在创面修复中的研究与应用进展

由各类因素所导致的急性、慢性、难愈性及医源性创面在临床上十分常见，常严重影响患者的生活质量并增加患者经济及社会医疗负担。随着细胞悬液移植相关的制备技术和载体材料研究趋渐成熟，其降低供皮区面积、治疗效果明确等优点使得自体/异体细胞应用于皮肤创面的治疗研究成为近年来创面修复临床研究的热点之一。但细胞悬液制备技术和载体技术目前尚无统一标准且部分技术尚未进行严格临床研究验证，仍需进行进一步临床研究明确其疗效及适应证。本文从细胞悬液的制备、移植及临床应用3个方面，对细胞悬液移植技术在创面修复方面的研究与应用进行归纳整理，以期为临床及科研工作者提供参考依据。     

碳酸钙口服混悬液治疗酸相关性疾病的多中心随机对照研究

目的 探讨碳酸钙口服混悬液与质子泵抑制剂在改善酸相关性疾病临床症状方面的疗效及安全性.方法 采用多中心随机对照研究方法,将400例14～70岁且至少有一个中度或重度症状(反酸、上腹痛、上腹不适、上腹烧灼感、上腹胀满)的酸相关性疾病患者均分为两组,分别予碳酸钙口服混悬液和奥美拉唑肠溶片治疗.观察所有患者第1次服药至临床症状缓解起效时间,服药后第3、7天临床症状改善情况,并记录患者的不良反应.统计学处理采用方差分析.结果 碳酸钙组患者第1次服药后反酸、上腹痛、上腹不适、上腹烧灼感、上腹胀满等症状的平均缓解起效时间明显快于奥美拉唑组(F=4.866、8.142、41.366、6.955、35.252,P均＜0.05).服药后第3天,碳酸钙组与奥美拉唑组的治疗有效率分别为69.50％、72.50％;服药后第7天,碳酸钙组与奥美拉唑组的治疗有效率分别为92.00％、96.50％.服药后第3、7天两组治疗有效率及综合疗效差异均无统计学意义(P均＞0.05).碳酸钙组中,轻度不良反应14例,中度不良反应2例;奥美拉唑组中,轻度不良反应10例.两组相比,差异无统计学意义(P＞0.05).结论 碳酸钙口服混悬液比质子泵抑制剂缓解酸相关性疾病症状更快速,能有效改善酸相关性疾病的症状.     

Release of Hydrocortisone from a Cream Matrix: Dependency of Release on Suspension Concentration and Measurement of Solubility and Diffusivity

The principal object of the present research was to investigate the sensitivity of drug release from a semisolid system to the manner of its preparation and to the concentration of drug placed within it. Established theory indicated that release should be concentration dependent, with the specific dependency determined by whether the drug in the system is fully in solution or is present substantially as suspended matter. To purposefully explore these relatively untested performance expectations, the total amount of drug in the formula was varied from a low concentration of 0.25% to a high concentration of 3%. Conditions were established such that release conformed to release from a semi-infinite medium into a receptor sink. At every concentration, release profiles were well reproduced in replicate samples and, where multiple lots of a kind were employed, from lot to identical lot. In all cases square root of time release kinetics were observed. Moreover and without exception, the square root of time release rate from run to run was directly proportional to the square root of the total concentration of hydrocortisone placed in the formulations. The amount released per square root of time per square root of total concentration was nearly identical from run to run irrespective of total concentration. The overall behavior fit theoretical expectations for suspensions having only a small fraction of the drug they contain in solution. That this condition prevailed even at the lowest 0.25% hydrocortisone strength was proven by independently measuring hydrocortisone's solubility (0.02%). Measurement of solubility permitted estimation of the effective diffusivity of the drug through the cream (2 × 10^?7 cm²/s).     

黄原胶在混悬剂和软膏中的应用

目的：考察黄胶在混悬剂和软膏中作为助,增稠剂的作用。方法：以炉甘石洗剂,联苯苄唑滴眼剂和联苯苄唑软膏作为模型,考察并比较不同天然胶在非均相液体制剂和半固体制剂中的作用;探讨黄原胶作为助悬剂和增稠剂的特点,结果：黄原胶具有触变性,助悬和增稠作用优于其它天然胶。结论：黄原胶是一种优良的助悬剂和增稠剂。     

Bullies, Victims and Bystanders: A Method of In-School Intervention and Possible Parental Contributions

This study developed a method of in-school intervention that dramatically reduced the suspension rate and violence in elementary schools. It suggests that children who were not read to by their parents often become bullies and/or victims of bullies. Other parental practices, including inconsistent discipline in the home, also may be contributing factors.