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排序方式: 共有118条查询结果,搜索用时 15 毫秒
1.
This work aims to provide a comprehensive study of the available research information on pesticide residues in honey through literature analysis. The research advancements within this research field from 1948 to 2019 are addressed using the Web of Science database. The results from the 685 articles analyzed indicate that this research field is in the focus of interest nowadays (Price index: 47.5%). The yearly production increased steadily from 2001 on, and authors, journals, and institutions followed Lotka’s law. On the other hand, Pico, Y (Spain) (2.5%), Journal of Chromatography A (5.8%), the USA (15.0%) and Agricultural Research Service (USA) (4.0%) were the most productive author, journal, country and institution, respectively. The research hotspots of this field, according to keyword analysis, are related to the chromatographic techniques for the determination of pesticides such as imidacloprid, neonicotinoids, or coumaphos in honey and derivate products such as propolis and wax.  相似文献   
2.
近年来随着农业生产的产业化,农药已经被大量的使用在农业生产中,但由于有些农药的监管能力不足或使用不当,使得农药残留问题十分严重。解决这个问题的关键就是提高和完善农药残留量的分析检测技术。在这个分析检测过程中,样品的前处理技术也是尤为的关键。文章综述了一些常用的样品前处理技术以及它们的最新研究进展,其中包括对固相微萃取、微波辅助萃取以及免疫亲和色谱技术等进行了逐一的介绍。文中不仅综述了农药残留分析技术未来的发展方向,也针对各类问题,提出了农药残留分析技术的挑战以及更高的要求。  相似文献   
3.
Pig feed may contain various levels of antimicrobial residues due to cross-contamination. A previous study showed that a 3% carry-over level of doxycycline (DOX) in the feed results in porcine faecal concentrations of approximately 4?mg/L.The aim of this study was to determine the effect of residual DOX concentrations (1 and 4?mg/L) in vitro on selection of DOX–resistant porcine commensal Escherichia coli and transfer of their resistance plasmids.Three different DOX–resistant porcine commensal E. coli strains and their plasmids were characterised. These strains were each brought in competition with a susceptible strain in a medium containing 0, 1 and 4?mg/L DOX. Resistant bacteria, susceptible bacteria and transconjugants were enumerated after 24?h and 48?h.The tet(A)–carrying plasmids showed genetic backbones that are also present among human E. coli isolates. Ratios of resistant to susceptible bacteria were significantly higher at 1 and 4?mg/L DOX compared with the blank control, but there was no significant difference between 1 and 4?mg/L. Plasmid transfer frequencies were affected by 1 or 4?mg/L DOX in the medium for only one of the resistance plasmids.In conclusion, DOX concentrations of 1 and 4?mg/L can select for resistant E. coli in vitro. Further research is needed to determine the effect of these concentrations in the complex environment of the porcine intestinal microbiota.  相似文献   
4.
目的:通过对胶体金试纸条的研究,并用该方法对硝基呋喃类代谢物残留量中呋喃妥因代谢物(AHD)进行测定。方法:用胶体金试纸法测定牛奶中硝基呋喃类代谢物残留量中呋喃妥因,与ELISA法进行比较。结果:本方法的测定结果与ELISA法检测结果相符,总符合率达100%;另外,两种方法的测定结果经配对t检验,结果差异无显著性(P>0.05);回收率在85%~92%之间,相对标准偏差14.5%。结论:胶体金试纸条法的建立与研究,并在牛奶中硝基呋喃类代谢物呋喃妥因残留量的应用;具有快速、准确、省时、灵敏与简便的特点。  相似文献   
5.
目的 建立栝楼桂枝汤纯化物中4种大孔树脂有机残留物的检测方法.方法 采用毛细管气相色谱法,测定栝楼桂枝汤纯化物中正己烷、苯、甲苯、二甲苯4种大孔树脂有机残留物的含量.结果 4种成分在相应线性范围内线性关系良好,r=0.9998~0.9999,平均回收率符合要求,各组分均未检出符合要求.HT5"H结论 该方法简便可行,精密度好,结果可靠,适用于栝楼桂枝汤纯化物中大孔树脂有机残留物的检测.  相似文献   
6.
氯霉素的毒性作用和检测方法研究进展   总被引:1,自引:0,他引:1  
氯霉素是一种广谱抗生素,因其抗菌效果好且价格低廉而被广泛用于畜牧养殖业,但对人体有不良反应,主要是引起人类骨髓功能抑制,从而导致再生障碍性贫血、血小板减少性紫癜等疾病.目前,我国已禁止在动物性食品生产过程中使用氯霉素,但私自使用的现象仍然存在,这就使氯霉素有可能残留在动物性食品中进而对人体健康构成危害.目前用于氯霉素残留的检测方法主要有:微生物法、免疫学法、气相色谱、液相色谱、气质联用及液质联用等,其中微生物及免疫学法常作为初步筛选实验;色谱法作为确证及定量方法;气质联用与液质联用为我国国标中氯霉素残留检测方法.  相似文献   
7.
汤军华  刘昭 《海峡药学》2011,23(4):68-69
目的对栽培桔梗进行分析测定,确定其是否具有农药的残留,以保证用药安全。方法采用气质联用法测定桔梗中残留的多种农药。不分流进样模式;进样口采用程序升温:120℃(4.5min)按80℃.min-1至250℃(34min);柱温箱程序升温:82℃(5min)按8℃.min-1至280℃;柱流速控制:恒压120Mpa;载气总流速:30mL.min-1;柱流速:1.75mL.min-1。MS离子源温度:200℃;接口温度:250℃;质谱检测模式:SIM。结果对栽培桔梗农药残留进行图谱分析,发现其含有农药敌敌畏、-αBHC、-δBHC、甲基对硫磷、二苯砜、毒死蜱等。结论栽培桔梗较野生桔梗,含有一定量的农药残留,在使用时应重视其质量问题。  相似文献   
8.
目的:建立了毛细管气相色谱法测定出口小麦粉中21种有机磷残留量的分析方法。方法:小麦粉经ASE溶剂萃取仪,丙酮加正己烷(1+1)溶剂提取,浓缩液丙酮溶解定容,采用HP50+毛细管柱和火焰光度检测器测定,一次进样可对小麦粉中21种有机磷农药进行检测。结果:样品加标平均回收率在74.3%~121.7%之间,相对标准偏差(RSD)2.1%~6.6%之间,检出限(LOD)在0.01 mg/kg~0.05 mg/kg之间。结论:实验结果表明,该法简便、快速、灵敏,准确,各项技术指标均满足农药残留检测的要求,适合出口小麦粉中21种有机磷残留量的日常检验。  相似文献   
9.
沈亦红 《医疗装备》2011,24(9):28-31
目的:建立顶空气相色谱法定性定量分析白内障超声乳化术中蓝域染色剂的化学助剂残留。方法:样品经80℃、30min静态顶空后,采用气相色谱-FID检测器进行检测,外标法定量。结果:加标回收试验结果表明,残留化学助剂加标回收率为89~95%,相对标准偏差均小于2%;方法的检出限为0.093~0.204μg/ml,定量下限为0.9~1.0μg/ml。结论:方法有效克服基体干扰,一次进样可同时分离和测定多种化学助剂,准确灵敏、简单快速,适用于白内障超声乳化术中蓝域染色剂的化学助剂的检测。  相似文献   
10.
There are now numerous in vitro and in silico ADME alternatives to in vivo assays but how do different industries incorporate them into their decision tree approaches for risk assessment, bearing in mind that the chemicals tested are intended for widely varying purposes? The extent of the use of animal tests is mainly driven by regulations or by the lack of a suitable in vitro model. Therefore, what considerations are needed for alternative models and how can they be improved so that they can be used as part of the risk assessment process? To address these issues, the European Partnership for Alternative Approaches to Animal Testing (EPAA) working group on prioritisation, promotion and implementation of the 3Rs research held a workshop in November, 2008 in Duesseldorf, Germany. Participants included different industry sectors such as pharmaceuticals, cosmetics, industrial- and agro-chemicals. This report describes the outcome of the discussions and recommendations (a) to reduce the number of animals used for determining the ADME properties of chemicals and (b) for considerations and actions regarding in vitro and in silico assays. These included: standardisation and promotion of in vitro assays so that they may become accepted by regulators; increased availability of industry in vivo kinetic data for a central database to increase the power of in silico predictions; expansion of the applicability domains of in vitro and in silico tools (which are not necessarily more applicable or even exclusive to one particular sector) and continued collaborations between regulators, academia and industry. A recommended immediate course of action was to establish an expert panel of users, developers and regulators to define the testing scope of models for different chemical classes. It was agreed by all participants that improvement and harmonization of alternative approaches is needed for all sectors and this will most effectively be achieved by stakeholders from different sectors sharing data.  相似文献   
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