高效液相色谱法测定银蒲解毒片中蒙花苷含量

目的建立银蒲解毒片中蒙花苷的含量测定方法。方法采用高效液相色谱法，Agilent Eclipse XDB-C18色谱柱（4．6mm×250mm，5μm），流动相为甲醇-4％冰醋酸水溶液（47：53），检测波长334nm。结果蒙花苷线性范围为2．24—44．8μg（r=0．9991）；平均回收率为97．8％，RSD=1．52％。结论方法可靠，简单可行，为控制银蒲解毒片的内在质量提供了依据。     

HPLC测定野菊花中两种黄酮类药效组分的含量

目的：建立并测定野菊花中两种黄酮类药效组分含量的方法。方法：以不同产地的供试品为载体,采用HPLC测定。Diamonsil C18色谱柱（4.6mm×250mm,5μm）;乙腈-0.5%磷酸水（乙腈起始浓度为10%,40min结束线性增加至50%）为流动相;检测波长为326nm;柱温30℃。结果：蒙花苷的线性范围为0.308~6.160μg（r=0.9999）,木犀草素-O-β-D-葡萄糖苷的线性范围为0.0732~1.4640μg（r=0.9999）,平均回收率分别为99.40%和99.67%,RSD分别为1.10%、0.83%（n=5）。结论：该方法同时测定2种有效成分,准确、快速、重现性好,适合于测定野菊花中蒙花苷和木犀草素-7-O-β-D-葡萄糖苷的含量。     

HPLC法测定咽炎颗粒中蒙花苷的含量

目的建立测定咽炎颗粒中蒙花苷含量的高效液相色谱法。方法采用高效液相色谱法。色谱柱：CAPCEll PAKC18（5μm,250mm×4.6mm）;流动相：甲醇-1%醋酸溶液（49∶51）;检测波长：334nm;流速：1.0ml/min;柱温：40℃。结果蒙花苷在4.64～69.6μg范围内呈良好的线性关系（r=0.9999）。蒙花苷的平均回收率为99.33%,RSD为0.79%（n=6）。结论该方法准确、灵敏度高,重复性好。     

Combined antihypertensive effect of luteolin and buddleoside enriched extracts in spontaneously hypertensive rats

Ethnopharmacological relevance

Flos Chrysanthemi is used in a variety of diseases in traditional Chinese medicine including hypertension, and the total flavonoids (rich in luteolin (LUT) and buddleoside (BUD)) of Flos Chrysanthemi is known to modulate vascular functions and reduce the blood pressure. However, the active flavonoids and their synergistic effects on anti-hypertension are still unclear. To investigate the combined anti-hypertension effects of LUT and BUD enriched extracts on spontaneously hypertensive rats (SHR), as well as the anti-hypertensive mechanism of LUT&BUD mixture.

Materials and methods

CODA Mouse & Rat Tail-Cuff Blood Pressure System was used to measure the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of SHR after treated with extracts contains with LUT and/or BUD. The expressions of Ang II, PRA, ALD, ET, PGI₂ and TXB₂ were investigated by ELASA. Serum NO concentration was measured by the method of Nitric acid reductase.

Results

A single administration of LUT, BUD, or LUT:BUD=1:1 significantly reduced SBP by about 3.35 mmHg, 4.39 mmHg and 15.42 mmHg, respectively. Chronic administration of LBM (at 60 mg/kg; p.o. for 30 days) reduced both SBP and DBP by 4.04% and 5.24% of the vehicle group, respectively. Oral administration of LBM at 60 mg/kg inhibited the serum levels of ANG, ALD and ET, but increased serum NO concentration.

Conclusion

This study shows the synergistic anti-hypertension effects of LUT and BUD in SHR. The effects of LBM on blood pressure are associated with RAAS and endothelial system. Thus, our experiments suggest that the combination of luteolin and buddleoside from Flos Chrysanthemi are potentially useful for the therapeutic treatments for hypertension.     

HPLC法测定消炎片中蒙花苷的含量

目的:建立反相高效液相色谱法测定消炎片中蒙花苷的含量。方法:采用AgiLent EcLipse XDB-C₁₈（250mm×4.6mm,5μm）色谱柱,以甲醇-乙腈-0.04%磷酸溶液为流动相,梯度洗脱;检测波长为334nm;流速1.0ml·min^-1;进样量10μl;柱温35℃。结果:蒙花苷的线性范围为0.0396～0.7927μg,r=1.0000（n=7）;提取回收率为98.76%,RSD为0.5%（n=6）。结论:本法操作简便、快速、结果准确,可用于消炎片的质量控制。     

败毒饮的薄层鉴别与含量测定

目的：建立败毒饮的质量标准。方法采用薄层色谱法对该制剂中赤芍、黄芩进行定性鉴别,采用 HPLC 同时对主要成分黄芩苷、蒙花苷进行定量测定。结果薄层鉴别色谱斑点清晰,分离度好,对照药材或对照品与阴性样品相比,在相应位置上无相同颜色斑点,阴性无干扰;黄芩苷和蒙花苷分别在0.2179～2.1790μg(r2＝0.9999)、0.1319～1.3190μg(r2＝0.9997)范围内线性关系良好。黄芩苷和蒙花苷加样回收率的RSD值分别为1.51%、2.01%。结论此方法简单方便,准确度及重复性好,可用于败毒饮的质量控制方法。     

感冒灵颗粒的质量标准研究

目的：对感冒灵颗粒建立质量标准。方法：对感冒灵颗粒方中马来酸氯苯那敏、对乙酰氨基酚、咖啡因、野菊花建立TLC鉴别，用HPLC对蒙花苷进行测定。结果：蒙花苷在0．12～5．95μg与峰面积呈良好的线性关系，平均加样回收率为100．91％。结沦：该法操作简便，结果准确，重复性好，可以控制感冒灵颗粒的质量。     

正交试验优化神香草中的迷迭香酸和蒙花苷提取工艺

目的 优化神香草中的迷迭香酸和蒙花苷提取条件,确定最佳的提取工艺。方法 在单因素试验的基础上,采用超声法提取神香草中的迷迭香酸和蒙花苷,选取提取溶剂、料液比、提取时间、提取次数为主要考察因素,采用L9(34)正交试验对迷迭香酸和蒙花苷提取工艺进行优化。结果 确定最佳提取条件,平行操作3次,神香草中迷迭香酸和蒙花苷的最佳提取工艺为提取溶剂为60%甲醇、料液比为1∶20、提取时间为20 min、提取次数为1次。结论 该方法操作便捷、快速,适用于神香草中的迷迭香酸和蒙花苷的提取。     

HPLC法同时测定鼻炎愈合剂中阿魏酸、绿原酸和蒙花苷含量

摘 要 目的：建立HPLC法测定鼻炎愈合剂中阿魏酸、绿原酸和蒙花苷含量。方法: 以Agilent ZORBAX SB C18柱（250 mm×4.6 mm,5 μm）为色谱柱,流动相为乙腈 0.1%磷酸溶液,梯度洗脱,流速为1.0 ml·min-1,柱温30℃,检测波长326 nm。结果: 绿原酸、阿魏酸、蒙花苷分别在3.039 7~30.396 8 μg·ml-1 (r=0.999 7),3.999 9~39.999 4 μg·ml-1 (r=0.999 9),5.820 7~58.207 2 μg·ml-1(r=0.999 9)范围内与峰面积呈良好线性关系,其平均加样回收率分别为98.92%,100.81%,99.89%,RSD分别为1.00%,1.02%,0.99%（n=6）。结论: 该方法操作简便、准确、重现性好,可用于鼻炎愈合剂的质量控制。     

密蒙花超声提取工艺研究

目的：采用正交实验设计确定密蒙花的最佳超声提取工艺。方法：采用单因素及正交实验设计,确定最佳工艺参数。结果：最佳提取工艺为用80％的乙醇超声提取20min,固液比为1：30。乙醇浓度对提取率影响最大,其次是固液比,提取时间影响最小。结论：与传统提取法相比,超声提取法能够提高蒙花苷的提取率,缩短提取时间,避免高温对黄酮类组分的破坏。本研究可为密蒙花的工业超声提取提供依据。